Egr transcription factors in the nervous system

Beckmann, Alison M.; Wilce, Peter A.

doi:10.1016/s0197-0186(96)00136-2

Cited by 320 publications

(308 citation statements)

References 195 publications

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“…The early growth response gene 1 (Egr-1), a member of the Egr family of genes that encodes for zinc-finger transcription factors, plays a role in the development, growth control and survival of several cell types, including T and B cells, neuronal cells and myeloid cells (Beckmann and Wilce, 1992;McMahon and Monroe, 1996;Krishnaraju et al, 1995Krishnaraju et al, , 1998. Egr-1 acts as a growth stimulator in human prostate tumors, but behaves as a tumor suppressor in other cells (Liu et al, 1999;Baron et al, 2003;Shafarenko et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Egr-1 abrogates the E2F-1 block in terminal myeloid differentiation and suppresses leukemia

2007

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Section: Introductionmentioning

confidence: 99%

Egr-1 abrogates the E2F-1 block in terminal myeloid differentiation and suppresses leukemia

2007

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“…Since their discovery in the late 1980s, cellular protooncogenes (Nishina et al, 1990;Sagar et al, 1988;Zerial et al, 1989) have become widely utilized tools to examine the effect of an acute stimulus; pharmacological or sensory (Beckmann and Wilce, 1997;Herdegen and Leah, 1998;Ziolkowska and Przewlocki, 2002). These transcription factors are also referred to as immediate-early genes (IEG) (Herrera and Robertson, 1996) and can be detected using immunohistochemistry (Dragunow and Faull, 1989) or in situ hybridization (Ziolkowska and Przewlocki, 2002) with members of the Fos-and Early growth response (Egr)-families being among the most extensively studied.…”

Section: Introductionmentioning

confidence: 99%

“…These transcription factors are also referred to as immediate-early genes (IEG) (Herrera and Robertson, 1996) and can be detected using immunohistochemistry (Dragunow and Faull, 1989) or in situ hybridization (Ziolkowska and Przewlocki, 2002) with members of the Fos-and Early growth response (Egr)-families being among the most extensively studied. Egr-1 (also known as zif 268, Krox-24, NGFI-A) has been extensively used to study neuronal responses to sensory stimuli and, unlike c-fos, exhibits a high basal expression (Beckmann and Wilce, 1997). Both IEGs are considered to reflect neuronal activity due to their function of controlling gene transcription via their protein forms binding to regulatory sites on DNA (Beckmann and Wilce, 1997;Chaudhuri, 1997;Herdegen and Leah, 1998;Hoffman and Lyo, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Egr-1 (also known as zif 268, Krox-24, NGFI-A) has been extensively used to study neuronal responses to sensory stimuli and, unlike c-fos, exhibits a high basal expression (Beckmann and Wilce, 1997). Both IEGs are considered to reflect neuronal activity due to their function of controlling gene transcription via their protein forms binding to regulatory sites on DNA (Beckmann and Wilce, 1997;Chaudhuri, 1997;Herdegen and Leah, 1998;Hoffman and Lyo, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Alterations in c-fos and Egr-1 (zif268) Expression Throughout the Rat Brain Following Acute Administration of Different Classes of Antidepressant Compounds

Slattery

Morrow²,

Hudson

et al. 2005

Neuropsychopharmacol

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The majority of immediate-early gene (IEG) studies focus on a few key brain regions associated with the class of psychoactive compound being studied. Recently, using a meta-analysis of the c-fos literature, we demonstrated the utility of c-fos profiling to classify such compounds. The present study examined acute delivery of a range of antidepressant classes; fluoxetine, imipramine, LiCl, and mirtazapine. The dual aims were to study the IEG profiles of these varying classes of antidepressants throughout the rat brain and to compare the utility of c-fos or Egr-1 as IEGs to classify clinically efficacious antidepressants. All antidepressants increased c-fos mRNA in the central amygdala, as previously shown, while c-fos was also increased in the anterior insular cortex and significantly decreased within the septum. Although acute antidepressant administration altered c-fos expression in a number of brain regions, Egr-1 expression was only significantly altered in the central amygdala, suggesting that Egr-1 may not be as useful a marker to investigate acute antidepressant treatment. The fact that these drugs, including the previously unclassified antidepressant mirtazapine, share a number of common loci of activation, which are implicated by human and animal studies in depression, adds further support to the use of IEG mapping to classify psychoactive compounds.

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“…Induction of the EGR transcription factor family in the nervous system has been linked to stress and stimuli[18]and the same conditions are known to initiates HSV-1 reactivation [19]. However, the roles of EGR in the regulation HSV-1 biology were not extensively studied.…”

Section: Discussionmentioning

confidence: 99%

Induction of Transcription Factor Early Growth Response Protein 1 during HSV-1 Infection Promotes Viral Replication in Corneal Cells

Hsia

Graham

Bedadala

et al. 2013

BMRJ

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Aims To understand the mechanisms of Early Growth Response Protein 1 (Egr-1) induction upon HSV-1 lytic infection and its roles in regulating viral gene expression and replication. Study Design Rabbit corneal cell line SIRC and other cell lines were infected by HSV-1 to investigate the Egr-1 induction and its occupancy on the viral genome in different conditions. UV-inactivated HSV-1 and a recombinant virus over-expressing Egr-1 were generated to evaluate the regulatory effects on viral gene expression and replication during the infection. Methodology Egr-1 induction triggered by viral infection was determined by Western Blot analyses and immune-fluorescent microscopy. Real-time RT-PCR and a novel Cignal™ Reporter Assay were used for quantitative measurement of Egr-1 expression. Chromatin Immuno-precipitation (ChIP) was performed to address the Egr-1 occupancy to the viral regulatory sequences and the influence on viral replication was assessed by plaque assays. Results Our results indicated that Egr-1 expression requires viral gene expression since the UV-inactivated HSV-1 failed to produce Egr-1 protein. Blockade of viral replication did not block the Egr-1 protein synthesis, supporting the hypothesis that HSV-1 replication was not essential for Egr-1 production. Chromatin immune-precipitation (ChIP) and RT-PCR assays demonstrated that induced Egr-1 was able to interact with key regulatory elements near HSV-1 immediate-early (IE) genes and promote viral gene expression. Recombinant virus overexpressing Egr-1 revealed that Egr-1 enhanced the viral replication and the release of infectious virus. Conclusion Together these results concluded that HSV-1 triggers the expression of an important host transcription factor Egr-1 via a unique mechanism and benefit the viral gene expression and replication.

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Egr transcription factors in the nervous system

Cited by 320 publications

References 195 publications

Egr-1 abrogates the E2F-1 block in terminal myeloid differentiation and suppresses leukemia

Egr-1 abrogates the E2F-1 block in terminal myeloid differentiation and suppresses leukemia

Comparison of Alterations in c-fos and Egr-1 (zif268) Expression Throughout the Rat Brain Following Acute Administration of Different Classes of Antidepressant Compounds

Induction of Transcription Factor Early Growth Response Protein 1 during HSV-1 Infection Promotes Viral Replication in Corneal Cells

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