EGR-1 and DUSP-1 are important negative regulators of pro-allergic responses in airway epithelium

Golebski, Korneliusz; Egmond, Danielle van; Groot, Esther J. de; Röschmann, Kristina; Fokkens, Wytske J.; Drunen, Cornelis M. van

doi:10.1016/j.molimm.2014.12.011

Cited by 18 publications

(14 citation statements)

References 40 publications

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“…We have previously demonstrated that the ATF-3, EGR-1, DUSP-1, and NFKB1 are affected by the airway epithelium exposure to a virus [ 17 ]. Moreover, EGR-1 and DUSP-1 play a critical role in down-regulating the virus-triggered inflammatory responses of epithelium [ 18 ]. Therefore, we sought to investigate whether deregulation of the expression of these transcription factors could potentially be responsible for the enhancement of TSLP production in epithelium isolated from CRSwNP tissue.…”

Section: Resultsmentioning

confidence: 99%

“…We have previously shown that negative regulators of inflammation EGR-1 and DUSP-1 display different levels of specificity depending on what triggers or what outcome measures are measured. In those experiments focusing on inflammatory responses we could show that a knock-down for EGR-1 affected IL-8 expression after house dust mite allergen (HDM) stimulation, but not after poly(I:C) induction, while a knock-down for DUSP-1 affected IL-6 and IL-8 after HDM or poly(I:C) stimulation [ 18 ]. In the present study, we show that the baseline DUSP-1 levels are lower in polyp epithelium than in healthy control group and that this may contribute to reduced polyp epithelial cell capability of tuning the viral-induced pro-Th2 inflammatory responses down.…”

Section: Discussionmentioning

confidence: 99%

“…The human airway epithelial cell line NCI-H292 (ATCC, USA) was cultured in RPMI 1640 medium supplemented with 10% (v/v) fetal calf serum (HyClone, USA), 1.25 mM of glutamine, 100 U/mL of penicillin, and 100 μg/mL of streptomycin. Creation of the EGR-1 and DUSP-1 knock-down mutants is described elsewhere [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…We have previously shown that the ATF-3, EGR-1, DUSP-1, and NFKB-1 transcription factors play an important role in poly(I:C) triggered inflammatory responses and that EGR-1 and DUSP-1 are responsible for a down-regulation of virus-induced inflammation in airway epithelium [ 17 , 18 ]. In allergic disease we demonstrated that deregulated basal expression levels of the ATF-3, EGR-1, DUSP-1, and NFKB1 genes were associated with differences in cytokine profile of nasal epithelial cells upon stimulation with an exogenous trigger [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

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Specific Induction of TSLP by the Viral RNA Analogue Poly(I:C) in Primary Epithelial Cells Derived from Nasal Polyps

et al. 2016

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IntroductionChronic rhinosinusitis with nasal polyposis is an inflammatory disease that, although not directly linked to allergy, often displays a Th2-skewed inflammation characterized by elevated local IgE and IL-5 levels. The nasal cavity is constantly exposed to bacteria and viruses that may trigger epithelial inflammatory responses. To gain more insight into mechanisms by which such a biased inflammation might arise, we have investigated the epithelial expression of the Th2 skewing mediators (TSLP, IL-25, and IL-33) in relationship to disease and microbial triggers.MethodsEpithelial cells were obtained from polyp tissues of nasal polyposis patients and from inferior turbinates of non-diseased controls. Cells were exposed to various TLR-specific triggers to study the effect on mRNA and protein expression level of TSLP, IL-25, and IL-33 and the potential regulatory mechanisms through the expression profile the transcription factors ATF-3, DUSP-1, EGR-1, and NFKB-1.ResultsThe TLR3 agonist and viral analogue poly(I:C) induced TSLP mRNA 13.0 ± 3.1 fold (p < 0.05) and protein expression by 12.1 ± 2.3-fold (p < 0.05) higher in epithelium isolated from nasal polyposis patients than in epithelium form healthy controls. This enhanced induction of TSLP may be a consequence of a down-regulated expression of DUSP-1 in polyp epithelium.ConclusionThe TLR3 induced expression of TSLP introduces a mechanism by which the Th2-skewed tissue environment might arise in nasal polyps and invites a further evaluation of the potential contribution of current or past viral infections to polyposis pathogenesis.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Specific Induction of TSLP by the Viral RNA Analogue Poly(I:C) in Primary Epithelial Cells Derived from Nasal Polyps

et al. 2016

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“…These transcription factors possess a basic leucine zipper (bZIP) domain that is essential for dimerization and DNA binding. The major subfamilies of AP-1 are Jun (c-Jun, JunB and JunD) and Fos (c-Fos, FosB, Fra1 and Fra2) proteins, which were first identified as the viral oncoproteins v-Fos and v-Jun in the Finkel-Biskis-Jinkins osteosarcoma virus and avian sarcoma virus, respectively [22].AP-1 has been implicated in a variety of tumoral processes, including inflammation, cell transformation, invasive growth, angiogenesis, and metastasis [23] .Like AP-1, ERG1 and DUSP1 are mainly activated by hypoxia, environmental stress, and proinflammatory cytokines [24,25].…”

Section: Discussionmentioning

confidence: 99%

Identification of Potential Non-invasive Biomarkers for Breast Cancer Prognosis and Treatment by Systematic Bioinformatics Analysis

He¹,

Wang²,

Guo³

2015

2015 7th International Conference on Information Technology in Medicine and Education (ITME)

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Objective: To observe the changes of gene expression in breast cancer stroma and peripheral blood mononuclear cells(PBMCs) of breast cancer patients. To investigate similarities and differences between them. Method: Datasets of gene expression profilings were downloaded from the Gene Expression Omnibus (GEO) database, including profilings of breast cancer vs. normal stroma and breast cancer patients' vs. healthy volunteers' PBMCs. BRB-ArrayTools was used to analyze the data to identify the differentiallyexpressed genes (DEGs) . Function of DEGs were annotated by the Database for Annotation, Visualization and Integrated Discovery (DAVID). Protein interaction analysis were then performed for the commonly deregulated genes. Results: 1565 and 1382 DEGs respectively were identified. Genes upregulated in the two dataset involved in biological processes, such as cell cycle, protein kinase cascade, negative regulation of programmed cell death, vasculature development.84 common genes were selected (74 up-and 10 down-regulated) to constructed the protein-protein interaction (PPI)network, from which the hub genes, including JUN,FOS,FOSB, early growth response 1 (EGR1), dual specificity phosphatase 1 (DUSP1)were extracted. Conclusion: The data suggests that gene expression pattern of these two profilings are similar at a certain degree. PBMCs maybe a better noninvasive material for biomarker detection of breast cancer.

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International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis

Wise

Lin

Toskala

et al. 2018

Int Forum Allergy Rhinol

339

453

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This critical review of the AR literature has identified several strengths; providers can be confident that treatment decisions are supported by rigorous studies. However, there are also substantial gaps in the AR literature. These knowledge gaps should be viewed as opportunities for improvement, as often the things that we teach and the medicine that we practice are not based on the best quality evidence. This document aims to highlight the strengths and weaknesses of the AR literature to identify areas for future AR research and improved understanding.

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EGR-1 and DUSP-1 are important negative regulators of pro-allergic responses in airway epithelium

Cited by 18 publications

References 40 publications

Specific Induction of TSLP by the Viral RNA Analogue Poly(I:C) in Primary Epithelial Cells Derived from Nasal Polyps

Specific Induction of TSLP by the Viral RNA Analogue Poly(I:C) in Primary Epithelial Cells Derived from Nasal Polyps

Identification of Potential Non-invasive Biomarkers for Breast Cancer Prognosis and Treatment by Systematic Bioinformatics Analysis

International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis

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