Egr-1 activation of rat adrenal phenylethanolamine N-methyltransferase gene.

Ebert, Steven N.; Balt, S. L.; Hunter, Julie; Gashler, Andrea; Sukhatme, Vikas P.; Wong, Dona L.

doi:10.1016/s0021-9258(17)31905-1

Cited by 91 publications

(2 citation statements)

References 55 publications

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“…17 PNMT, for example, is a catecholamine biosynthetic enzyme that is modulated by cortisol and the GR, 37,38 but the transcription factor EGR-1 is equally vital in its expression. 39,40 We have shown that PNMT is markedly downregulated in LTH adrenal medullary tissue despite no GR differences, 41 and the expression of EGR-1 has been shown to vary with acute hypoxia. 42 This implies that LTH may modulate EGR-1 and other transcription factor expression levels in the adrenal medulla as well as the cortex.…”

Section: Discussionmentioning

confidence: 79%

Expression and Distribution of Glucocorticoid Receptors in the Ovine Fetal Adrenal Cortex: Effect of Long-term Hypoxia

et al. 2008

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This study was designed to determine if long-term hypoxia (LTH) alters adrenal glucocorticoid receptor (GR) expression in the ovine fetal adrenal cortex. Ewes were maintained at 3820 m from approximately 30 to 138 to 140 days' gestation, and fetal adrenals were collected. Western analysis revealed two approximately 94-kDa GR-alpha isoforms and a lower molecular weight (45 kDa) form. A decreasing trend in the ratio of 94-kDa/45-kDa bands following LTH suggested an increase in GR turnover. Immunohistochemistry demonstrated dense GR staining in the zona glomerulosa with minimal staining in the zona fasciculata in the control group, while dense staining was observed throughout the cortex in LTH. Western analysis and reverse transcription polymerase chain reaction confirmed that the GR- beta isoform is not present or expressed at extremely low levels in the fetal adrenal, hypothalamus, pituitary, and placenta. These data indicate that LTH alters GR-alpha function in the fetal adrenal cortex and suggest that GR-beta is not expressed in sheep.

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Section: Discussionmentioning

confidence: 79%

Expression and Distribution of Glucocorticoid Receptors in the Ovine Fetal Adrenal Cortex: Effect of Long-term Hypoxia

et al. 2008

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“…In den Motiven M7 und M14 liegen Octamer- Da in M4 das nicht-Octamer-Motiv TAATGARAT ohne mismatch vorkommt und eine Bindung von Oct-1 an das TAATGARAT-Motiv bereits in anderen Kontexten nachgewiesen wurde (Baumruker et al, 1988;Bendall et al, 1993;Herr und Cleary, 1995), wurde die Beziehung zwischen M4 und Oct-1 näher untersucht und wird hier exemplarisch dargestellt. Genen besonders hervor (Ebert et al, 1994;Carrasco-Serrano et al, 1998;Nagavarapu et al, 2001;Tai et al, 2006).…”

Section: Bindung Der Fplc-aufgereinigten Proteine An Dynabeadsunclassified