Eggs and salmonella food-poisoning: an evaluation

Duguid, J. P.; North, Richard

doi:10.1099/00222615-34-2-65

Cited by 68 publications

(29 citation statements)

References 27 publications

(6 reference statements)

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“…Various serotypes of Salmonella are implicated in food-borne infections. In particular, after the 1980s, the serotype Enteritidis has caused infection via contaminated eggs in many countries (2,12,21,23). Hens infected with serotype Enteritidis produce eggs whose contents are contaminated with the microorganism (1,20).…”

mentioning

confidence: 99%

Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates

Ohtsuka¹,

Yanagawa²,

Takatori

et al. 2005

Appl Environ Microbiol

137

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Loop-mediated isothermal amplification (LAMP) assay was effective in detecting Salmonella enterica in naturally contaminated liquid egg samples. Salmonella was detected in 110 samples taken from four eggbreaking plants. The egg samples were pre-enriched in buffered peptone water (BPW) at 37°C for 20 h. The selective enrichment was done in Rappaport-Vassiliadis or tetrathionate broth and plated onto xylose lysine deoxycholate agar and brilliant green agar, modified. In addition, the PCR assay was used to detect Salmonella after pre-enrichment in BPW at 37°C for 20 h. The culture method and PCR assay were compared to the LAMP assay, which was also performed after pre-enrichment in BPW. PCR failed to detect Salmonella in 10% of 110 samples, whereas the culture method and LAMP assay successfully identified Salmonella in all samples. However, the LAMP assay was found to be much more rapid than the culture method and as sensitive in detecting Salmonella from liquid eggs. In all of the egg-breaking plants studied, Salmonella was isolated on most tested days. The positive samples showed that more than 75% of the Salmonella strains had identical genetic patterns when analyzed by pulsed-field gel electrophoresis. This suggests that the same Salmonella strains having survived long periods of time in the plants were contaminating the production line. The LAMP assay is rapid, specific, and sensitive for Salmonella detection in liquid eggs and is able to monitor Salmonella contamination in egg-handling plants more reliably.

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mentioning

confidence: 99%

Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates

Ohtsuka¹,

Yanagawa²,

Takatori

et al. 2005

Appl Environ Microbiol

137

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“…Survival of salmanella in poultry houses and reinfection of new, salmonella-free stock has been associated with wild mice and birds and human carriers (Duguid & North, 1991). On the farms investigated, S. typhimurium was not eliminated until eradication of the beetle Alphitobius diaperinus was complete.…”

Section: Figure 2 Restriction Profiles Of Salmonella Typhimurium Orimentioning

confidence: 99%

“…At the farm, the infection can be introduced by one-day-old chickens, feed, personnel, wild birds, rodents or it can persist in the environment and reinfect new, salmonella-free chickens. In spite of intensive cleaning and disinfection, it is very difficult to eliminate salmonella bacteria from an infected farm (Duguid & North, 1991).…”

Section: Introductionmentioning

confidence: 99%

Plasmid profiles and phage types ofSalmonella typhimuriumisolated from successive flocks of chickens on three parent stock farms

1992

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SUMMARYThree-hundred-and-eighty-seven strains of Salmonella typhimurium obtained from successive generations of parent stock originating from three different rearing farms were characterized by phage typing and plasmid profiling. Seventy-six strains representing dominant types were selected for restriction enzyme analysis and colony hybridization.The main phage type on each of the three farms was 110. Plasmid profiling, however, allowed further subtyping. All but three isolates carried the serotype-specific virulence-associated plasmid. Restriction enzyme analysis showed variations in this plasmid as well as the presence of co-migrating plasmids of the same size.At each locality one or more clonal lines of S. typhimurium were «isolated from successive generations, indicating that the infections were persistent.Although house construction, sanitation and disinfection procedures, in addition to biosecurity in general, were improved significantly during the observation period, infection with 5. typhimurium was not eliminated until eradication of the beetle Alphitobius diaperinus was complete.

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“…infection was reported in one of 10,000 eggs, and Salmonella spp. infection was identified in one of 15,000 eggs in the United Kingdom (Duguid and North, 1991). In the United Kingdom, where an average person eats three raw eggs per week, 1 in 100 people was found to be infected with Salmonella and suffer from food poisoning every year (Duguid and North, 1991).…”

Section: Introductionmentioning

confidence: 99%

First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea

Kim¹,

Song²,

Lim³

et al. 2013

Korean Journal for Food Science of Animal Resources

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Salmonella Enteritidis is responsible for causing foodborne diseases upon consumption of egg products. While cases of S. Enteritidis isolation from eggs have been reported in other countries, no such cases have previously been reported in Korea. In this study, we report the first isolation and identification of S. Enteritidis from domestically distributed eggs in Korea. Eggs were collected from eight countrywide grocery stores during different seasons between 2011 and 2012. Egg contents and washing solution of egg shells were incubated in buffered peptone water, and the enriched broth was further enriched in tetrathionate broth and Rappaport-Vassiliadis. The secondary enriched broth was streaked on xylose lysine desoxycholate agar. The suspected colonies were confirmed to S. Enteritidis by a biochemical test, serotyping, and PCR test. Genetic relatedness among the isolates was analyzed using Diversilab Salmonella kit. Three strains of S. Enteritidis were isolated from egg contents and egg shells collected from grocery stores of the Eumseong-city in the fall of 2011. All three stains showed resistance to chloramphenicol, streptomycin, nalidixic acid, and ampicillin by the disk diffusion method. In addition, the isolates showed more than 99% DNA homology, indicating that they were presumably identical strains. Therefore, there is a requirement to monitor and control against S. Enteritidis from eggs in Korea.

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Eggs and salmonella food-poisoning: an evaluation

Cited by 68 publications

References 27 publications

Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates

Detection of Salmonella enterica in Naturally Contaminated Liquid Eggs by Loop-Mediated Isothermal Amplification, and Characterization of Salmonella Isolates

Plasmid profiles and phage types ofSalmonella typhimuriumisolated from successive flocks of chickens on three parent stock farms

First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea

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