Egg-yolk protein by-product as a source of ACE-inhibitory peptides obtained with using unconventional proteinase from Asian pumpkin (Cucurbita ficifolia)

Eckert, Ewelina; Zambrowicz, Aleksandra; Pokora, Marta; Setner, Bartosz; Dąbrowska, Anna; Szołtysik, Marek; Szewczuk, Zbigniew; Polanowski, Antoni; Trziszka, Tadeusz; Chrzanowska, Józefa

doi:10.1016/j.jprot.2014.08.003

Cited by 49 publications

(31 citation statements)

References 26 publications

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“…The peptides obtained by hydrolysis with unconventional aspartyl proteinase from Y. lipolytica JII1c were characterized by much lower free radical scavenging activity (about 4.5-5.5 times lower than activity of other hydrolysates) and ACE-inhibitory activity. Similarly to our results, hydrolysis of the egg-yolk protein by-product with using various proteases including neutrase, porcine pepsin and protease from C. ficifolia, led to the production of peptides with varied antioxidant and antihypertensive activity (Zambrowicz et al 2015a, b;Eckert et al 2014;Pokora et al 2013). In addition, simultaneous ability of enzymatic hydrolysates to scavenge of DPPH radicals and to inhibit of ACE have been demonstrated in other study (Davalos et al 2004;Lu et al 2010;Zambrowicz et al 2015b).…”

Section: Preparation Of Peptides With Biological Activity From the Egsupporting

confidence: 84%

“…Furthermore, we have proven that peptides: LAPSLPGKPKPD and VTGRFAGHPAAQ possessed antidiabetic activity, as demonstrated by significant inhibition of α-glucosidase activity in vitro (Zambrowicz et al 2015a, b). We confirmed the multidirectional biological activity of synthetic analogs of YP-derived peptides (Zambrowicz et al 2015a, b;Eckert et al 2014).…”

Section: Introductionsupporting

confidence: 81%

“…Considerable research has been conducted to develop methods for the production and isolation of bioactive peptides from delipidated egg YP-the main by-products of lecithin extraction from hen egg yolks, as have been already documented by our research team (Eckert et al 2014;Zambrowicz et al 2015a, b). The usage of proteinaceous by-products for preparation of bioactive peptides by enzymatic hydrolysis may reduce the cost of peptide production and provide an interesting method of waste disposal.…”

Section: Introductionmentioning

confidence: 95%

“…In our previous study, we demonstrated strong antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activity of peptides generated and isolated from the peptic hydrolysate (YINQMPQKSRE, YINQMPQKSREA, VTGRFAGHPAAQ, YIEAVNKVSPRAGQF) and obtained after hydrolysis with protease from Cucurbita ficifolia (AGTTCLFTPLALPYDYSH, LAPSLPGKPKPD, RASD-PLLSV RNDDLNYIQ). (Zambrowicz et al 2015a, b;Eckert et al 2014). Furthermore, we have proven that peptides: LAPSLPGKPKPD and VTGRFAGHPAAQ possessed antidiabetic activity, as demonstrated by significant inhibition of α-glucosidase activity in vitro (Zambrowicz et al 2015a, b).…”

Section: Introductionmentioning

confidence: 99%

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Production and Identification of Biologically Active Peptides Derived from By-product of Hen Egg-Yolk Phospholipid Extraction

Eckert

Zambrowicz

Bobak

et al. 2018

Int J Pept Res Ther

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Biologically active peptides derived from food proteins have been increasingly popular due to their therapeutic properties. This paper attempts to utilize a by-product of phospholipid extraction from egg yolk as a source of peptides with antioxidant [radical scavenging capacity, Fe 2+ chelating effect, reducing power (FRAP)] and ACE-inhibitory activity. In addition, in this research we analysed the ability to release bio-peptides of a non-commercial proteases obtained from Yarrowia lipolytica yeast. Yeast serine protease exert greater ability to evaluate antioxidant and ACE inhibitory activity than yeast aspartyl protease during degradation of protein by-product. Purification procedures including membrane filtration, gel filtration chromatography and reversed-phase high-performance liquid chromatography led to obtain a decapeptide QSLVSVPGMS with strong DPPH free radical scavenging and ACE inhibitory activities. A new application of the serine protease from Y. lipolytica yeast in the production of bioactive peptides from egg yolk protein by-product was demonstrated.

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Section: Preparation Of Peptides With Biological Activity From the Egsupporting

confidence: 84%

Section: Introductionsupporting

confidence: 81%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production and Identification of Biologically Active Peptides Derived from By-product of Hen Egg-Yolk Phospholipid Extraction

Eckert

Zambrowicz

Bobak

et al. 2018

Int J Pept Res Ther

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“…21 Finally, it is very important to determine the cytotoxic activity of potential nutraceuticals or/and pharmacological substances to establish their safety and proper impact on the body. 21 Finally, it is very important to determine the cytotoxic activity of potential nutraceuticals or/and pharmacological substances to establish their safety and proper impact on the body.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and antidiabetic activities of peptides isolated from a hydrolysate of an egg-yolk protein by-product prepared with a proteinase from Asian pumpkin (Cucurbita ficifolia)

et al. 2015

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Bioactive peptides derived from food have been increasingly popular due to their therapeutic properties. Of particular importance are peptides with a multidirectional activity that can be used in the treatment and prevention of diet-related diseases. This paper attempts to utilize a by-product of phospholipid extraction from egg yolk as a source of peptides with a broad spectrum of biological activity. In addition, in this research we used a non-commercial enzyme obtained from Asian pumpkin, which has not been sufficiently researched in terms of its ability to release biopeptides from food proteins. In the present study the biological properties of peptides, derived from egg-yolk protein by-products (YP) remaining after phospholipid extraction, and their four synthetic analogs were investigated with regard to their antioxidant (radical scavenging capacity, Fe 2+ chelating effect, reducing power (FRAP)) and antidiabetic (a-glucosidase and DPP-IV inhibitory activities) properties. One of them, with the sequence LAPSLPGKPKPD, exhibited the highest antioxidant activity (free radical scavenging activity (6.03 mM Trolox eq per mg protein); FRAP (296.07 mg Fe 2+ per mg protein)). This peptide also revealed the strongest DPP-IV (361.5 mmol L À1 ) and a-glucosidase (1065.6 mmol L À1 ) inhibitory activities, a novel multifunctional effect for peptides from an egg-yolk hydrolysate.

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Utilization of Egg Byproducts for Food and Biomaterial Applications

Tsopmo

Tsige

Udenigwe

2019

Byproducts From Agriculture and Fisheries

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Egg-yolk protein by-product as a source of ACE-inhibitory peptides obtained with using unconventional proteinase from Asian pumpkin (Cucurbita ficifolia)

Cited by 49 publications

References 26 publications

Production and Identification of Biologically Active Peptides Derived from By-product of Hen Egg-Yolk Phospholipid Extraction

Production and Identification of Biologically Active Peptides Derived from By-product of Hen Egg-Yolk Phospholipid Extraction

Antioxidant and antidiabetic activities of peptides isolated from a hydrolysate of an egg-yolk protein by-product prepared with a proteinase from Asian pumpkin (Cucurbita ficifolia)

Utilization of Egg Byproducts for Food and Biomaterial Applications

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