Egg yolk oils exert anti-inflammatory effect via regulating Nrf2/NF-κB pathway

Xiao, Nanhai; Zhao, Yan; Wen, He; Yao, Yao; Wu, Na; Xu, Mingsheng; Du, Hongwu; Tu, Yonggang

doi:10.1016/j.jep.2021.114070

Cited by 13 publications

(12 citation statements)

References 39 publications

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“…KEGG analysis demonstrated that OS focused on responses to nutrients and hyperoxia, and gastrointestinal functions, including pancreatic secretion, and Cb contributed to re-epithelization by regulating tissue structure homeostasis, which was conducive to the integrity of the endothelial barrier. Consistent with OS and Cb, GB has a historical application in the treatment of ulcers and inflammatory disorders due to its anti-inflammatory, detoxifying, and anti-oxidant effects ( Mahmoudi et al, 2013 ; Pan et al, 2016 ; Gao et al, 2020 ; Xiao et al, 2021 ), as well as the wound healing–promoting property ( Salari et al, 2016 ; Yoo et al, 2017 ). The targets of GB had a strong correlation with inflammatory reactions, such as TNF, IL-17, HIF-1 signaling pathways, carcinogenesis, and VEGF-regulated migration, as well as responses to oxidative stress, toxic substance, and chemical stress.…”

Section: Discussionmentioning

confidence: 99%

“…The dosage of DC in the CW formula for adults was 0.3 g/d, which is far less than the recommended level 3–6 g/d in Chinese Pharmacopoeia 2020 ( Huang et al, 2021 ) and hence resolves the concern regarding toxicity ( Bei-Xuan et al, 2016 ). The potential hepatoxicity of SR ( Yang et al, 2021 ) and FS might be counteracted by the detoxifying effect of GB ( Mahmoudi et al, 2013 ; Pan et al, 2016 ; Gao et al, 2020 ; Xiao et al, 2021 ). As a result, 16S rRNA gene sequencing results confirmed that the CW therapy inhibited the prevalence of Shigella sonne , which contributes to drug resistance ( Starling, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

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The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation

Shao

et al. 2022

Front. Pharmacol.

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Inflammatory bowel disease (IBD) is pathologically characterized by an immune response accommodative insufficiency and dysbiosis accompanied by persistent epithelial barrier dysfunction. The Cao-Xiang-Wei-Kang (CW) formula has been utilized to treat gastrointestinal disorders in the clinic. The present study was designed to delineate the pharmacological mechanisms of this formula from different aspects of the etiology of ulcerative colitis (UC), a major subtype of IBD. Dextran sodium sulfate (DSS) was given to mice for a week at a concentration of 2%, and the CW solution was administered for 3 weeks. 16S rRNA gene sequencing and untargeted metabolomics were conducted to examine the changes in the microbiome profile, and biochemical experiments were performed to confirm the therapeutic functions predicted by system pharmacology analysis. The CW treatment hampered DSS-induced experimental colitis progression, and the targets were enriched in inflammation, infection, and tumorigenesis, which was corroborated by suppressed caspase 3 (Casp3) and interleukin-1b (IL-1b) and increased cleaved caspase 3 expression and casp-3 activity in the colon samples from colitis mice subjected to the CW therapy. Moreover, the CW therapy rescued the decreased richness and diversity, suppressed the potentially pathogenic phenotype of the gut microorganisms, and reversed the altered linoleic acid metabolism and cytochrome P450 activity in murine colitis models. In our in vitro experiments, the CW administration increased the alternative activation of macrophages (Mφs) and inhibited the tumor necrosis factor-α (TNFα)-induced reactive oxygen species (ROS) level and subsequent death in intestinal organoids (IOs). We propose that the CW formula alleviates the progression of murine colitis by suppressing inflammation, promoting mucosal healing, and re-establishing a microbiome profile that favors re-epithelization.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation

Shao

et al. 2022

Front. Pharmacol.

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“…Then, the RT-PCR was conducted in a CFX Connect Real-time System (Bio-Rad, USA). RT-PCR conditions for amplification consist of denaturation at 94 °C for 10 s, anealing at 60 °C for 15 s, and extension at 72 °C for 10 s. The whole reaction was maintained for 40 amplification cycles . The target gene expression was normalized to GAPDH gene expression, and the relative gene expression was evaluated using the 2 –ΔΔCt method .…”

Section: Materials and Methodsmentioning

confidence: 99%

“…The WST-1 method was used to detect cell viability. 27 Briefly, after the cell supernatant was collected, each well was washed three times with a phosphate-buffered solution (PBS). Then, the WST-1 reagent was diluted with RPMI 1640 (1:30, v/v) containing 5% FBS and added to each well and then incubated at 37 °C for 10−30 min.…”

Section: Methodsmentioning

confidence: 99%

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Huang

Chen

Yao

et al. 2023

J. Agric. Food Chem.

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Ovotransferrin (OVT) has been confirmed to have anti-inflammatory activity. However, its effect and mechanism on gastric inflammation are unclear. In this study, the effect and mechanism of the OVT on the tumor necrosis factor-α (TNF-α) induced inflammatory response in gastric epithelial cells (GES-1) were investigated. The enzyme linked immunosorbent assay (ELISA) was used to determine the levels of inflammation cytokines, followed by RNA sequencing to explore the potential pathways of its anti-inflammatory effect, and then it was validated by Western blotting and pathways inhibitors. Results showed that the OVT at concentrations of 50–400 μg/mL displayed nontoxicity against GES-1 cells. Additionally, 100 μg/mL of OVT obviously reduced the secretion of interleukin (IL)-8, IL-6, and TNF-α by 63.02% (630.09/1703.98), 35.53% (935.81/1451.43), and 36.19% (964.60/1511.63), respectively. The results of RNA sequencing exhibited that the OVT significantly influences the activation of mitogen-activated protein kinase (MAPK) and the nuclear factor kappa-light-chain enhancer of activated B cell (NF-κB) pathways, which was verified by the levels of p-IKK, p-IκB, p-P65, p-ERK, p-JNK, and p-P38 protein. IL-8 contents released by GES-1 cells after incubation with inhibitors of NF-κB and MAPK pathways further confirmed that OVT hindered activation of these two pathways. Collectively, these results suggested that OVT was a natural protein with the potential to treat gastric inflammation.

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“…As part of the reaction, RT-PCR was carried out using start TM Green qPCR Super Mix (TransGen Biotech, Beijing, China), including denaturation at 94 °C for 5 s, annealing at 60 °C for 15 s, and extension at 72 °C for 10 s. The entire reaction was kept for 40 amplification cycles. The expression levels of the target genes were normalized to GAPDH and the 2 −ΔΔCt method was used for calculating the relative expression levels of target genes, 23 as shown in Table 2.…”

Section: Rt-pcr Analysismentioning

confidence: 99%

Ovotransferrin alleviated acute gastric mucosal injury in BALB/c mice caused by ethanol

et al. 2023

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Egg yolk oils exert anti-inflammatory effect via regulating Nrf2/NF-κB pathway

Cited by 13 publications

References 39 publications

The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation

The Cao-Xiang-Wei-Kang formula attenuates the progression of experimental colitis by restoring the homeostasis of the microbiome and suppressing inflammation

Ovotransferrin Inhibits TNF-α Induced Inflammatory Response in Gastric Epithelial Cells via MAPK and NF-κB Pathway

Ovotransferrin alleviated acute gastric mucosal injury in BALB/c mice caused by ethanol

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