Egg White Lysozyme Promoted Collagen Secreting of Dermal Fibroblasts in Mice

Han, Haiyan; Li, X.; Yu, Kang-Yeol; Ma, W.Z.; Cao, Zhichen; An, Guijie; Lian, Z.X.

doi:10.3923/ajava.2011.667.677

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…First, the enriched carboxyl and hydroxyl groups on the film as characterized by Raman and XPS (Figure 1, Figures S1 and S2) had great potential to bind effectively with calcium ions 67 from the medium for osteoblasts culture. Second, it is previously found that lysozyme could promote the secretion of collagen in dermal fibroblasts and induce the synthesis of ECM proteins in the dermis of mice, 68 and both of these events are important for the growth of osteoblasts. Intact antifouling behavior was helpful to produce repeatable antimicrobial activity.…”

Section: ■ Introductionmentioning

confidence: 99%

An Environmentally Benign Antimicrobial Coating Based on a Protein Supramolecular Assembly

Liu

et al. 2016

ACS Appl. Mater. Interfaces

175

135

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The use of antimicrobial materials, for example, silver nanoparticles, has been a cause for concern because they often exert an adverse effect on environmental and safety during their preparation and use. In this study, we report a class of green antimicrobial coating based on a supramolecular assembly of a protein extracted from daily food, without the addition of any other hazardous agents. It is found that a self-assembled nanofilm by mere hen egg white lysozyme has durable in vitro and in vivo broad-spectrum antimicrobial efficacy against Gram-positive/negative and fungi. Such enhanced antimicrobial capability over native lysozyme is attributed to a synergistic combination of positive charge and hydrophobic amino acid residues enriched on polymeric aggregates in the lysozyme nanofilm. Accompanied with high antimicrobial activity, this protein-based PTL material simultaneously exhibits the integration of multiple functions including antifouling, antibiofilm, blood compatibility, and low cytotoxicity due to the existence of surface hydration effect. Moreover, the bioinspired adhesion mediated by the amyloid structure contained in the nanofilm induces robust transfer and self-adhesion of the material onto virtually arbitrary substrates by a simple one-step aqueous coating or solvent-free printing in 1 min, thereby allowing an ultrafast route into practical implications for surface-functionalized commodity and biomedical devices. Our results demonstrate that the application of pure proteinaceous substance may afford a cost-effective green biomaterial that has high antimicrobial activity and low environmental impact.

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Section: ■ Introductionmentioning

confidence: 99%

An Environmentally Benign Antimicrobial Coating Based on a Protein Supramolecular Assembly

Liu

et al. 2016

ACS Appl. Mater. Interfaces

175

135

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“…Retinoic acid can inhibit the induction of c-Jun protein by UV irradiation, thereby preventing increased matrix metalloproteinase activity and the ensuing dermal damage [1]. Moreover, Lys can raise the moisture and collagen content of mouse dermis by inducing the expression level of collagen and hyaluronic acid [37]. For the present 5-week animal experiments, the recovery rates of photoaged skin were higher in all Ada-treated mice than in the control group at all treatment time points.…”

Section: Plos Onementioning

confidence: 59%

Application of ultrasound-mediated adapalene-coated lysozyme-shelled microbubbles in UVA-induced skin photoaging

et al. 2020

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Photoaging, the premature aging of skin induced by ultraviolet rays, is characterized by wrinkling, roughness, laxity, and pigmentary changes. Various natural and synthetic retinoids have been explored for the treatment of aging. Among retinoids, adapalene (Ada, 0.3%) is one of the most potent and widely used drugs to treat photoaging. However, it causes irritant reactions that limit its acceptance by patients. Several studies have shown the applicability of Lysozyme (Lys)-shelled microbubbles (MBs) for drug delivery through sonophoresis, and recently we have shown its efficiency to treat inflammatory skin disease. Here, we report the construction of novel Ada-LysMBs based on opposite electric charges for combined effects to treat photoaging. The Ada-LysMBs were self-assembled and had a mean diameter of 2857 nm. The maximum loading efficiency of Ada onto LysMBs was 13.99 ± 0.59%. An acoustic power density of 3 W/cm 2 for 1 min revealing maximum penetration depth of LysMBs was optimized for further in vitro and in vivo studies of Ada-LysMBs. It was observed that in vitro Ada release from Ada-LysMBs at 6 h after ultrasound (US) treatment was more rapid at pH 7.4 (82%) than at pH 5.5 (73%). Franz diffusion experiments on isolated porcine skin indicated that US approximately doubled Ada delivery by Ada-LysMBs and Ada + LysMBs at 12 h and six-fold Lys permeation by LysMBs at 6 h, compared to these treatments alone. A 5-week in vivo study in mice identified significant wrinkle reduction in animals treated with US plus Ada-LysMBs. Our findings indicate that US may be used with Ada-LysMBs in the water phase to treat photoaging by normalizing hyperkeratinization and promoting collagen synthesis.

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“…8 Lysozymes in EW have antibacterial properties and can hydrolyze specific polysaccharides that make up the cell walls of some Gram-positive bacteria. 9–12 Owing to their biodegradability, natural and synthetic polymers play essential roles in drug delivery, wound care, scaffold, dental, and cardiovascular applications. 13…”

Section: Introductionmentioning

confidence: 99%

“…8 Lysozymes in EW have antibacterial properties and can hydrolyze specic polysaccharides that make up the cell walls of some Grampositive bacteria. [9][10][11][12] Owing to their biodegradability, natural and synthetic polymers play essential roles in drug delivery, wound care, scaffold, dental, and cardiovascular applications. 13 Advanced materials design in bone tissue engineering requires a combination of different materials to obtain superior properties (physiochemical, mechanical, and biological properties).…”

Section: Introductionmentioning

confidence: 99%

The enhanced properties and bioactivity of poly-ε-caprolactone/poly lactic-co-glycolic acid doped with carbonate hydroxyapatite–egg white

Patty,

Nugraheni,

Ana

et al. 2023

RSC Adv.

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Egg White Lysozyme Promoted Collagen Secreting of Dermal Fibroblasts in Mice

Cited by 7 publications

References 27 publications

An Environmentally Benign Antimicrobial Coating Based on a Protein Supramolecular Assembly

An Environmentally Benign Antimicrobial Coating Based on a Protein Supramolecular Assembly

Application of ultrasound-mediated adapalene-coated lysozyme-shelled microbubbles in UVA-induced skin photoaging

The enhanced properties and bioactivity of poly-ε-caprolactone/poly lactic-co-glycolic acid doped with carbonate hydroxyapatite–egg white

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