Egg cylinder development during in vitro extended embryo culture predicts the post transfer developmental potential of mouse blastocysts

Logsdon, Deirdre M.; Ermisch, Alison F; Kile, Rebecca; Schoolcraft, William B.; Krisher, Rebecca L.; Yuan, Ye

doi:10.1007/s10815-020-01714-9

Cited by 6 publications

(4 citation statements)

References 12 publications

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“…Previous studies has indicated that TE biopsy significantly increases the risk of monozygotic splitting in utero [12,[53][54] . However, there are very few studies on human embryo splitting in vitro, and most of them are based on in vitro-matured oocytes, which may not reflect physiological conditions [55] . The biological mechanisms and consequences contributing to embryo splitting in humans are poorly understood, and there are ethical and safety concerns regarding the potential risks of embryo splitting for pregnancy outcomes and the health of offspring [52] .…”

Section: Embryo Splitting Due To Te Biopsymentioning

confidence: 99%

Fate of human twin embryos generated unintentionally in in vitro fertilization: Clinical and ethical perspectives

Kinoshita,

Bhor

2024

Int. J. Clin. Obstet. Gynaecol.

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In vitro embryo splitting or twinning is a method of reproductive cloning that involves dividing a single early-stage embryo into two or more individual cells and growing them as identical embryos. The first successful intentional in vitro human embryo splitting, reported in 1993, sparked a heated ethical discussion on the cloning of human embryos. Later, the intricacies of the ethical debate related to cloning through human embryo splitting were globally recognized. In response to this, several countries and international organizations have implemented prohibitions either through legal statutes, official decrees, or public statements. However, most of the studies reported the post-implantation spontaneous embryo splitting in utero that results into monozygotic twins or multiples and aren't fall under the prohibitions of cloning. Recently, rare cases of unintentional in vitro embryo splitting were confirmed during their culture mostly due to naturally zona-free oocytes or zona manipulations. Due to the ambiguity of current legislation to discriminate between intentional or unintentional in vitro embryo splitting, the fate of those embryos to transfer or not, remains a question. After assessing the potential risk associated with, there is a need to reconsider the prohibitions imposed on transfer of unintentionally generated split embryos in poor responders and advanced maternal age patients undergoing IVF treatment, where the use of donor gametes is also prohibited. This review paper describes the various IVF laboratory procedures that rarely and unintentionally generates split embryos. Moreover, the cellular and molecular changes occurring in split embryos and ethical considerations about use of split embryos to transfer are also deliberated.

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Section: Embryo Splitting Due To Te Biopsymentioning

confidence: 99%

Fate of human twin embryos generated unintentionally in in vitro fertilization: Clinical and ethical perspectives

Kinoshita,

Bhor

2024

Int. J. Clin. Obstet. Gynaecol.

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“…The method of human extended embryo culture has been previously described (14,16). In brief, after transport and storage in either the study system or storage in LN 2 dewars, the embryos were warmed using the Kitazato warming protocol and left to recover for 2 hours in 20-mL drops of Sage BM medium with 10% serum protein substitute under oil in 21% O 2 and 7.5% CO 2 to maintain a medium pH between 7.2 and 7.3 (16).…”

Section: Blastocyst Extended Culturementioning

confidence: 99%

“…The imaging experiments were performed using a 3i Marianas inverted spinning disk confocal microscope as previously described (14). The images were then analyzed for the epiblast cell number using the surface tool and object identification within the surface of approximately 5-10 mM in diameter using Imaris x64 9.20 software.…”

Section: Embryo Immunofluorescence Staining and Confocal Analysismentioning

confidence: 99%

“…A novel extended in vitro embryo culture system was developed to support peri-implantation stage human embryo development without maternal input (12,13). Recently, this system was shown to predict the posttransfer developmental potential of mouse blastocysts (14). Preliminary work on human blastocysts also suggested that peri-implantation development during in vitro extended culture may reflect the human blastocyst developmental potential after embryo transfer (15).…”

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confidence: 99%

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Evaluation of the TMRW vapor phase cryostorage platform using reproductive specimens and in vitro extended human embryo culture

et al. 2021

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Objective: To assess the impact of shipment and storage of sperm, oocytes, and blastocysts in vapor phase nitrogen compared with static storage in liquid phase nitrogen. Design: Prospective cohort-matched study. Setting: Multiple in vitro fertilization laboratories in an in vitro fertilization network. Patient(s): Fifty-eight human embryos, 32 human oocytes, 15 units of bovine semen. Intervention(s): Vapor vs. liquid nitrogen. Main Outcome Measure(s): The postwarming survival of oocytes, sperm, and blastocysts, and the developmental potential of blastocysts during in vitro extended culture. Result(s): Custom-designed labware, for use with the TMRW platform, enables continuous temperature monitoring during shipment and/or storage in the vapor phase robotic storage system. The highest temperature recorded for specimens shipped to a domestic laboratory was À180.2 C with a mean AE SD of À190.4 AE 0.5 C during shipment and À181.1 AE 0.6 C during storage. Likewise, specimens shipped internationally had a high of À180.2 C with a mean AE SD of À193.5 AE 0.6 C during shipment and À181.2 AE 0.7 C during storage. Results from the extended culture assays have revealed no deleterious effect of shipment and storage in nitrogen vapor. The viability of mammalian gametes and embryos was equivalent between the vapor phase and liquid phase storage. Conclusion(s):The evaluated system did not have any deleterious effects on the postwarming survival of sperm, oocytes, and blastocysts. The postwarming developmental potential of human blastocysts during in vitro extended culture was unaffected by storage and handling in the vapor phase nitrogen TMRW platform when compared with static liquid phase nitrogen storage. Our results suggest that the vapor phase cryostorage platform is a safe system to handle and store reproductive specimens for human assisted reproductive technology. (Fertil Steril Sci Ò 2021;2:268-77. Ó2021 by American Society for Reproductive Medicine.

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Estrogen signaling encourages blastocyst development and implantation potential

Logsdon

Churchwell

Schoolcraft

et al. 2023

J Assist Reprod Genet

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Egg cylinder development during in vitro extended embryo culture predicts the post transfer developmental potential of mouse blastocysts

Cited by 6 publications

References 12 publications

Fate of human twin embryos generated unintentionally in in vitro fertilization: Clinical and ethical perspectives

Fate of human twin embryos generated unintentionally in in vitro fertilization: Clinical and ethical perspectives

Evaluation of the TMRW vapor phase cryostorage platform using reproductive specimens and in vitro extended human embryo culture

Estrogen signaling encourages blastocyst development and implantation potential

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