EGG CELL 1 contributes to egg-cell-dependent preferential fertilization in Arabidopsis

Wang, Wei; Malka, Raphael; Lindemeier, Maria; Cyprys, Philipp; Tiedemann, Sophie; Sun, Kaiting; Zhang, Xuecheng; Xiong, Hanxian; Sprunck, Stefanie; Sun, Meng-Xiang

doi:10.1038/s41477-023-01616-5

Cited by 2 publications

(1 citation statement)

References 84 publications

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“…During the final stage of fertilization, thionins secreted from pollen tubes promote synergid cell degeneration (20) (11). EC1 peptides from egg cells promote gamete fusion (21) (22) and ECA1 gametogenesisrelated family proteins (23) also play a role in fertilization. RALF6/7/16/36/37 secreted from pollen tubes (24) and ECS1/2 secreted from ovules (25) (26) are involved in blocking polytubey to accomplish fertilization.…”

Section: Introductionmentioning

confidence: 99%

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Matsuura-Tokita,

Suzuki,

Kimata

et al. 2024

Preprint

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Brassinosteroids (BRs) are steroid hormones identified in plants. Besides promoting cell elongation and division, BRs facilitate the development of both male and female reproductive tissues. In animals, reproductive steroid hormones play an essential role in reproductive tissue development by regulating gene expression. Here, we focused on the function of BRs during fertilization. We measured the content of biologically active BRs, brassinolide (BL) and castasterone (CS), in the reproductive tissues of Arabidopsis thaliana. Both BL and CS accumulated abundantly in pollen grains and in larger amounts in pistils than in leaves. To evaluate BL function during fertilization, we used an in vitro guidance assay with exogenously applied BL. Although pollen tubes need to be elongated through the pistils for efficient capacitation, BL treatment promoted pollen tube capacitation and improved attraction to ovules in vitro. Transcriptome analysis demonstrated that BL treatment induced the expression of half of the genes expressed in pollen tubes that elongated through the pistils. These results indicated that BL supplied from pistils is a key factor for pollen tube capacitation. However, using the bri1 mutant for the guidance assay resulted in reduced pollen tube capacitation, suggesting that BRI1-signaling in pistils is also important. Furthermore, BRs act on ovules. Exogenous BL application to ovules maintained guidance capacity by promoting the expression of small secreted proteins involved in pollen tube attraction and gamete fusion. Overall, BRs play a significant role as male and female reproductive hormones throughout the plant fertilization process.

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Section: Introductionmentioning

confidence: 99%

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Matsuura-Tokita,

Suzuki,

Kimata

et al. 2024

Preprint

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Development of a Versatile System to Facilitate Targeted Knockout/Elimination Using CRISPR/Cas9 for Highly Duplicated Gene Families inArabidopsisSexual Reproduction

Takeuchi,

Nagahara

2024

Preprint

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CRISPR/Cas9-based targeted gene editing is a fundamental technique for studying gene functions in various organisms. In plants, the introduction of a T-DNA construct harboring Cas9 nuclease and single guide RNA (sgRNA) sequences induces sequence-specific DNA double-strand breaks, inducing the loss of gene function.Arabidopsis thalianais a model for CRISPR/Cas9 system development and gene function studies; the introduction of Cas9 under the egg or zygote promoter and multiple sgRNA modules generates heritable or non-mosaic mutants for multiple targets in the T1 generation ofA. thaliana. Recent reports reflect use of several CRISPR/Cas9 vectors in generating single- and higher-order mutants; however, the development of a reliable, cost-effective, and high-throughput CRISPR/Cas9 platform is necessary for targeting highly duplicated gene families. In this study, we have developed a simple and user-friendly construction system for the CRISPR/Cas9 vector series with improved gene editing efficiency by simply inserting a single intron intoCas9, and effectively demonstrated the simultaneous knockout of multiple genes involved inA. thalianasexual reproduction. An unbiased PCR-mediated mutant identification in the T1 generation revealed that our CRISPR/Cas9 system can support a > 70 kb deletion of > 30 tandemly duplicated synergid-specific genes and simultaneous knockout of five redundant genes essential for double fertilization. We performed a one-shot knockout of seven homologous pollen tube receptor-like kinase genes and identified their specific and overlapping roles in pollen tube growth and guidance. Our system can potentially facilitate further research in experimental plant biology to search for genetically unidentified components using reverse genetic candidate approaches.

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EGG CELL 1 contributes to egg-cell-dependent preferential fertilization in Arabidopsis

Cited by 2 publications

References 84 publications

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression

Development of a Versatile System to Facilitate Targeted Knockout/Elimination Using CRISPR/Cas9 for Highly Duplicated Gene Families inArabidopsisSexual Reproduction

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