EGFR-mediated intracellular delivery of Pc 4 nanoformulation for targeted photodynamic therapy of cancer: in vitro studies

Master, Аlyssa M.; Qi, Yue; Oleinick, Nancy L.; Gupta, Anirban Sen

doi:10.1016/j.nano.2011.09.012

Cited by 70 publications

(60 citation statements)

References 51 publications

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“…Recently, a novel 12 amino acids peptide, GE11, has been shown to target EGFR receptor in vitro and in vivo and mediate cellular uptake of nanocarriers [17,18].…”

Section: Introductionmentioning

confidence: 99%

EGFR targeted thermosensitive liposomes: A novel multifunctional platform for simultaneous tumor targeted and stimulus responsive drug delivery

Haeri

Zalba

Hagen

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…Recently, a novel 12 amino acids peptide, GE11, has been shown to target EGFR receptor in vitro and in vivo and mediate cellular uptake of nanocarriers [17,18].…”

Section: Introductionmentioning

confidence: 99%

EGFR targeted thermosensitive liposomes: A novel multifunctional platform for simultaneous tumor targeted and stimulus responsive drug delivery

Haeri

Zalba

Hagen

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…GE11 is a novel peptide first reported on by Li et al in 2005, 18 and its high affinity for certain EGFR-positive tumor cells has been verified ex vitro and in vivo. 19,20 However, the potential for use of this peptide as a targeting ligand to achieve targeted drug delivery to tumors, especially NSCLC, has not as yet been reported.…”

Section: Introductionmentioning

confidence: 99%

GE11-modified liposomes for non-small cell lung cancer targeting: preparation, ex vitro and in vivo evaluation

Cheng¹,

Huang²,

Liu³

et al. 2014

IJN

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Non-small cell lung cancer (NSCLC) is a serious threat to human health, and 40%–80% of NSCLCs express high levels of epidermal growth factor receptor (EGFR). GE11 is a novel peptide and exhibits high affinity for EGFR binding. The aim of this study was to construct and evaluate GE11-modified liposomes for targeted drug delivery to EGFR-positive NSCLC. Doxorubicin, a broad-spectrum antitumor agent, was chosen as the payload. GE11 was conjugated to the distal end of DSPE-PEG 2000 -Mal by an addition reaction with a conjugation efficiency above 90%. Doxorubicin-loaded liposomes containing GE11 (GE11-LP/DOX) at densities ranging from 0% to 15% were prepared by combination of a thin film hydration method and a post insertion method. Irrespective of GE11 density, the physicochemical properties of these targeted liposomes, including particle size, zeta potential, and drug entrapment efficiency, were nearly identical. Interestingly, the cytotoxic effect of the liposomes on A549 tumor cells was closely related to GE11 density, and liposomes with 10% GE11 had the highest tumor cell killing activity and a 2.6-fold lower half maximal inhibitory concentration than that of the nontargeted counterpart (PEG-LP/DOX). Fluorescence microscopy and flow cytometry analysis revealed that GE11 significantly increased cellular uptake of the liposomes, which could be ascribed to specific EGFR-mediated endocytosis. It was found that multiple endocytic pathways were involved in entry of GE11-LP/DOX into cells, but GE11 assisted in cellular internalization mainly via the clathrin-mediated endocytosis pathway. Importantly, the GE11-modified liposomes showed enhanced accumulation and prolonged retention in tumor tissue, as evidenced by a 2.2-fold stronger mean fluorescence intensity in tumor tissue than the unmodified liposomes at 24 hours. In summary, GE11-modified liposomes may be a promising platform for targeted delivery of chemotherapeutic drugs in NSCLC.

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“…51 EGFR-specific peptide GE-111 peptide and PS (Pc4) were tested successfully against HER2-specific cells using polymeric nanoparticles and PDT. 52 5-ALA attached to dendrimers by ester bonds was more efficient on A431 cell lines compared to free 5-ALA. 22 Third-generation aryl ether dendritic porphyrins, dendrimers of porphyrin monomers, were found to be very efficient in PDT applications. 53 Several studies used phage display methods to identify efficient tumor-targeting peptides.…”

mentioning

confidence: 91%

Photosensitizer and peptide-conjugated PAMAM dendrimer for targeted in vivo photodynamic therapy

Amreddy

Vijayashree

Adimoolam

et al. 2015

IJN

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Challenges in photodynamic therapy (PDT) include development of efficient near infrared-sensitive photosensitizers (5,10,15,20-tetrakis(4-hydroxyphenyl)-21 H ,23 H -porphine [PS]) and targeted delivery of PS to the tumor tissue. In this study, a dual functional dendrimer was synthesized for targeted PDT. For targeting, a poly(amidoamine) dendrimer (G4) was conjugated with a PS and a nitrilotriacetic acid (NTA) group. A peptide specific to human epidermal growth factor 2 was expressed in Escherichia coli with a His-tag and was specifically bound to the NTA group on the dendrimer. Reaction conditions were optimized to result in dendrimers with PS and the NTA at a fractional occupancy of 50% and 15%, respectively. The dendrimers were characterized by nuclear magnetic resonance, matrix-assisted laser desorption/ionization, absorbance, and fluorescence spectroscopy. Using PS fluorescence, cell uptake of these particles was confirmed by confocal microscopy and fluorescence-activated cell sorting. PS-dendrimers are more efficient than free PS in PDT-mediated cell death assays in HER2 positive cells, SK-OV-3. Similar effects were absent in HER2 negative cell line, MCF-7. Compared to free PS, the PS-dendrimers have shown significant tumor suppression in a xenograft animal tumor model. Conjugation of a PS with dendrimers and with a targeting agent has enhanced photodynamic therapeutic effects of the PS.

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EGFR-mediated intracellular delivery of Pc 4 nanoformulation for targeted photodynamic therapy of cancer: in vitro studies

Cited by 70 publications

References 51 publications

EGFR targeted thermosensitive liposomes: A novel multifunctional platform for simultaneous tumor targeted and stimulus responsive drug delivery

EGFR targeted thermosensitive liposomes: A novel multifunctional platform for simultaneous tumor targeted and stimulus responsive drug delivery

GE11-modified liposomes for non-small cell lung cancer targeting: preparation, ex vitro and in vivo evaluation

Photosensitizer and peptide-conjugated PAMAM dendrimer for targeted in vivo photodynamic therapy

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