1987
DOI: 10.1152/ajprenal.1987.253.2.f299
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EGF-induced mitogenesis in proximal tubular cells: potentiation by angiotensin II

Abstract: The mitogenic effect of epidermal growth factor (EGF) and the characteristics of EGF binding were studied on primary cultures of rabbit proximal tubular cells. EGF was found to be a potent mitogen and stimulated DNA synthesis 18-fold above the level observed in quiescent cells. Using 125I-EGF as a ligand, two classes of specific EGF receptors were identified on the proximal tubular cell in culture, i.e., a high-affinity receptor with a dissociation constant (Kd) of 0.3 nM and maximal binding (Bmax) of 1.7 X 10… Show more

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Cited by 113 publications
(88 citation statements)
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“…Taken together, these data are analytic and pharmacologic evidence for a contribution of PGE 2 to EGF-mediated stimulation of basolateral organic anion uptake. In addition, EGF-stimulated PGE 2 release has been described in proximal tubular cells before (45). We therefore conclude that EGF induces prostaglandin formation in OK cells and that PGE 2 is probably one of the active metabolites.…”
Section: Discussionsupporting
confidence: 62%
“…Taken together, these data are analytic and pharmacologic evidence for a contribution of PGE 2 to EGF-mediated stimulation of basolateral organic anion uptake. In addition, EGF-stimulated PGE 2 release has been described in proximal tubular cells before (45). We therefore conclude that EGF induces prostaglandin formation in OK cells and that PGE 2 is probably one of the active metabolites.…”
Section: Discussionsupporting
confidence: 62%
“…It modifies fluid and electrolyte reabsorption (Cogan, 1990;Harris, 1992) and exerts metabolic effects including gluconeogenesis and ammonia production (Chobanian and Julin, 1987;Goligorsky et al, 1987;Johnston et al, 1993). In vitro ATII induces hypertrophy of proximal tubular cells (Wolf and Neilson, 1990;Harris, 1992;Wolf et al, 1993;Wolf, 1993) and potentiates the mitogenic effects of epidermal growth factor (Norman et al, 1987). It also induces the cellular oncogenes c-myc, c-fos, and c-Nras (Wolf and Neilson, 1990), as well as growth factors such as TGF-1 (Wolf et al, 1995).…”
Section: Discussionmentioning
confidence: 99%
“…Further, EGF has been shown to stimulate phosphorylation and to inhibit gluconeogenesis in rat proximal tubule cells [159]. Finally, the EGF-induced mitogenesis in proximal tubular cells is enhanced by angiotensin II [161], thereby suggesting a possible interaction between the EGF and the renin angiotensin systems.…”
Section: In Vitro Evidence For Egf Effects On Kidney Cellsmentioning
confidence: 96%