EGF–FGF2 stimulates the proliferation and improves the neuronal commitment of mouse epidermal neural crest stem cells (EPI-NCSCs)

Bressan, Raul Bardini; Melo, Fernanda Rosene; Almeida, Patricia Alves de; Bittencourt, Denise Avani; Visoni, Silvia Beatriz Coutinho; Jeremias, Talita Silva; Costa, Ana Paula; Leal, Rodrigo Bainy; Trentin, Andréa Gonçalves

doi:10.1016/j.yexcr.2014.05.020

Cited by 29 publications

(16 citation statements)

References 26 publications

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“…This capacity is of interest, since in pathological conditions the replacement of neurons may be optimized by acting simultaneously on various processes 38, 39 . The effect of the β-carbolines on cellular proliferation and differentiation is not unique to these compounds, having been observed for endogenous molecules such as leukotriene B4 40 , BMPs 41 , the growth factors EGF/FGF2 42 , and NGF/BDNF/bFGF 43 , and the transcription factors Lmx1a and Lmx1b 44 . However, the fact that the β-carbolines also stimulated migration highlights the versatility of these exogenous compounds, as they can promote the three processes involved in full adult neurogenesis.…”

Section: Discussionmentioning

confidence: 99%

The alkaloids of Banisteriopsis caapi, the plant source of the Amazonian hallucinogen Ayahuasca, stimulate adult neurogenesis in vitro

Morales-García

Revenga

Alonso‐Gil

et al. 2017

Sci Rep

118

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Banisteriopsis caapi is the basic ingredient of ayahuasca, a psychotropic plant tea used in the Amazon for ritual and medicinal purposes, and by interested individuals worldwide. Animal studies and recent clinical research suggests that B. caapi preparations show antidepressant activity, a therapeutic effect that has been linked to hippocampal neurogenesis. Here we report that harmine, tetrahydroharmine and harmaline, the three main alkaloids present in B. caapi, and the harmine metabolite harmol, stimulate adult neurogenesis in vitro. In neurospheres prepared from progenitor cells obtained from the subventricular and the subgranular zones of adult mice brains, all compounds stimulated neural stem cell proliferation, migration, and differentiation into adult neurons. These findings suggest that modulation of brain plasticity could be a major contribution to the antidepressant effects of ayahuasca. They also expand the potential application of B. caapi alkaloids to other brain disorders that may benefit from stimulation of endogenous neural precursor niches.

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Section: Discussionmentioning

confidence: 99%

The alkaloids of Banisteriopsis caapi, the plant source of the Amazonian hallucinogen Ayahuasca, stimulate adult neurogenesis in vitro

Morales-García

Revenga

Alonso‐Gil

et al. 2017

Sci Rep

118

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“…Genetic factors, cytokine imbalance, and oxidative stress can cause abnormal hair follicle cycling and subsequent hair loss (Rho et al, 2005;Aron et al, 2013). Many cytokines and receptors are involved in the cell cycle of human follicle dermal papilla cells, including the vascular endothelial cell growth factor (VEGF) (Shin et al, 2014), vascular endothelial cell growth factor receptor (VEGFR) (Li et al, 2012), fibroblast growth factor (FGF) (Rho et al, 2005), insulin-like growth factor (IGF) (Panchaprateep and Asawanonda, 2014), epidermal growth factor (EGF) (Bressan et al, 2014), keratinocyte growth factor (KGF) (Gopu et al, 2015), and transforming growth factor (TGF) (Kang et al, 2013;Shin et al, 2014). Some hair regeneration has been achieved by molecular effect and growth factors (Danilenko et al, 1996) and follicle dermal stem cells (Rahmani et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Enhanced VEGF Expression in Hair Follicle Dermal Papilla Cells by Centella asiatica Linn.

Saansoomchai¹,

Limmongkon²,

Surangkul³

et al. 2018

CMUJNS

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Centella asiatica Linn. (C. asiatica) extract has been shown to possess high antioxidant activity due to its phenols and flavonoids. This study tested the efficacy of 70%-ethanol (EtOH) crude extracts of C. asiatica and its fractions (H 2 O, EtOAc, CH 2 Cl 2 , and hexane) to modulate human follicle dermal papilla cells. In addition, we analyzed the extracts for major phytochemicals as well as free radical scavenging activity. Our results from ABTS and DPPH assays showed that the amounts of phenolic and flavonoid compounds in the extracts were both related to its free radical scavenging activity. While the EtOAc fraction of C. asiatica demonstrated the highest free radical scavenging activity, it was toxic to human follicle dermal papilla cells. The cell viability test was positive when cells were treated with EtOH crude extract and H 2 O fraction. VEGF gene expression, quantified by real-time PCR analysis of the EtOH crude extract, showed a significant level of induction, indicating that the growth promotion effect in human follicle dermal papilla cells was related to VEGF gene expression, which has a positive hair growth stimulating effect. The EtOH crude extract of C. asiatica may offer potential in hair growth promoting products.

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“…Alternatively, serum may promote commitment of NC cells to neuronal fate, which may be accompanied by downregulation of Sox10 expression. 23,24 It would be interesting to address these two hypotheses and identify the pathways that may contribute to maintenance of the NC Our results clearly showed that FGF2 was sufficient to induce Sox10 expression even in the absence of serum, to the same extent as the complete medium. FGF2 was also necessary, as blocking FGFR signaling completely eliminated Sox10 and NES expression as well as NC induction altogether.…”

Section: Discussionmentioning

confidence: 86%

“…Interestingly, the presence of serum reduces the fraction of Sox10+/NES+ cells to less than 60% but maintains the same level of FoxD3+/NES+ cells, suggesting that serum may contain inhibitors of Sox10 expression. Alternatively, serum may promote commitment of NC cells to neuronal fate, which may be accompanied by downregulation of Sox10 expression . It would be interesting to address these two hypotheses and identify the pathways that may contribute to maintenance of the NC phenotype so as to enable long‐term expansion and use of these cells for regenerative medicine.…”

Section: Discussionmentioning

confidence: 99%

Derivation of neural crest stem cells from human epidermal keratinocytes requires FGF‐2, IGF‐1, and inhibition of TGF‐β1

Tseropoulos

Boroujeni

Bajpai

et al. 2018

Bioengineering & Transla Med

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Neural crest (NC) cells play a central role in forming the peripheral nervous system, the craniofacial skeleton, and the pigmentation of the skin during development due to their broad multilineage differentiation potential into neurons, Schwann cells, melanocytes, and mesenchymal stem cells. Recently, we identified an easily accessible source of pluripotent NC stem cells from human inter‐follicular keratinocyte (KC) cultures (KC‐NC). In this work, we examined specific conditions for the derivation of NC from KC cultures. More specifically, we examined the role of two growth factors, FGF2 and IGF1, in NC proliferation and in expression of two potent NC transcription factors, Sox10 and FoxD3. Using specific chemical inhibitors, we uncovered that the downstream regulatory pathways AKT/PI3K, MEK/ERK, and JNK/cJun may be critical in Sox10 and FoxD3 regulation in KC‐NC. The TGF‐β1 pathway was also implicated in suppressing Sox10 expression and NC proliferation. In summary, our study shed light into the role of FGF2, IGF1, and TGF‐β1 on the induction of NC from KC cultures and the pathways that regulate Sox10 and FoxD3. We also established culture conditions for sustaining KC‐NC multipotency and, therefore, the potential of these cells for regenerative medicine and cellular therapies.

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EGF–FGF2 stimulates the proliferation and improves the neuronal commitment of mouse epidermal neural crest stem cells (EPI-NCSCs)

Cited by 29 publications

References 26 publications

The alkaloids of Banisteriopsis caapi, the plant source of the Amazonian hallucinogen Ayahuasca, stimulate adult neurogenesis in vitro

The alkaloids of Banisteriopsis caapi, the plant source of the Amazonian hallucinogen Ayahuasca, stimulate adult neurogenesis in vitro

Enhanced VEGF Expression in Hair Follicle Dermal Papilla Cells by Centella asiatica Linn.

Derivation of neural crest stem cells from human epidermal keratinocytes requires FGF‐2, IGF‐1, and inhibition of TGF‐β1

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