EGF-dependent lobule formation and FGF7-dependent stalk elongation in branching morphogenesis of mouse salivary epithelium in vitro

Morita, Kuniharu; Nogawa, Hiroyuki

doi:10.1002/(sici)1097-0177(199906)215:2<148::aid-dvdy7>3.0.co;2-v

Cited by 71 publications

(54 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, submandibular epithelium synthesizes a heparinbinding EGF-like growth factor in vivo, which appears to be involved in epithelial branching (Umeda et al, 2001). Furthermore, it is noteworthy that the effective concentration of EGF decreased to one tenth in the presence of FGF7 (Morita and Nogawa, 1999). In addition, the commercially available, regular bovine serum albumin (BSA; not fatty acidfree) used for cell culture may be contaminated with a trace amount of LPA, because LPA binds to serum albumin (Tigyi and Miledi, 1992).…”

Section: Discussionmentioning

confidence: 99%

“…Our previous report demonstrated that most bromodeoxyuridine (BrdU) -labeled cells detected at 8 hr after epithelium-alone culture represented the cell population that the mesenchyme had stimulated before culture and that had not terminated DNA synthesis, and that not until 12 hr after cultivation did most of the BrdUlabeled cells result from the stimulation of EGF in the medium (Morita and Nogawa, 1999). For this study, we investigated how DNA-synthesizing cells at 12 hr and 24 hr of culture differed among three culture conditions: EGF and LPA, EGF alone, and LPA alone.…”

Section: Proliferating Cellsmentioning

confidence: 99%

“…Epithelium-alone cultures were made according to the method of our previous study (Morita and Nogawa, 1999). Epithelia were embedded in growth Fig.…”

Section: Epithelium-alone Culturementioning

confidence: 99%

“…Cultured epithelia were labeled with BrdU for 1 hr at 11 to 12 hr or 23 to 24 hr of cultivation and were processed according to the protocol for wholemount preparations used in our previous study (Morita and Nogawa, 1999).…”

Section: Detection Of Proliferating Cellsmentioning

confidence: 99%

See 3 more Smart Citations

Lysophosphatidic acid cooperates with EGF in inducing branching morphogenesis of embryonic mouse salivary epithelium

Noguchi¹,

Okamoto²,

Kasama³

et al. 2005

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

Epithelial morphogenesis is supported by diffusible growth factors and by nondiffusible cell substrata, such as laminin and fibronectin. When embedded in a laminin-rich basement-membrane substratum, embryonic mouse submandibular epithelium undergoes cell proliferation and branching morphogenesis in response to epidermal growth factor (EGF) in mesenchyme-free culture but not in serum-free medium. In this study, we sought to identify the biologically active factor in serum. As this factor was heat-stable and trypsinresistant, the lipid fraction was analyzed. Horse serum was fractionated by ethanol extraction, Folch partition with chloroform-methanol-water, and high-performance liquid chromatography, and we tested the branch-inducing activity of each fraction. We also analyzed the partially purified fraction with a mass spectrometer, indicating that the active fraction largely consisted of lysophosphatidyl-hexose. Finally we identified the molecule as lysophosphatidic acid (LPA), because, whereas lysophosphatidyl-inositol had only a slight branch-inducing activity, its relevant LPA fully substituted for serum and induced branching morphogenesis in cooperation with EGF. LPA receptor genes were expressed in submandibular epithelial cells. DNA-synthesizing cells were abundant only when cultured in the presence of both EGF and LPA, but not either singly. Developmental Dynamics 235:403-410, 2006.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Proliferating Cellsmentioning

confidence: 99%

“…Epithelium-alone cultures were made according to the method of our previous study (Morita and Nogawa, 1999). Epithelia were embedded in growth Fig.…”

Section: Epithelium-alone Culturementioning

confidence: 99%

Section: Detection Of Proliferating Cellsmentioning

confidence: 99%

See 2 more Smart Citations

Lysophosphatidic acid cooperates with EGF in inducing branching morphogenesis of embryonic mouse salivary epithelium

Noguchi¹,

Okamoto²,

Kasama³

et al. 2005

Developmental Dynamics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Branching morphogenesis of ex vivo SMGs involves both FGFR signaling (Hoffman et al, 2002;Jaskoll et al, 2002;Morita and Nogawa, 1999) and EGFR signaling (Kashimata and Gresik, 1997;Kashimata et al, 2000). Previously, we identified a role for FGFR1 and phosphatidylinositol 3-kinase (PI3K) in SMG branching morphogenesis (Larsen et al, 2003).…”

Section: Introductionmentioning

confidence: 95%

FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis

et al. 2005

View full text Add to dashboard Cite

Branching morphogenesis of mouse submandibular glands is regulated by multiple growth factors. Here, we report that ex vivo branching of intact submandibular glands decreases when either FGFR2 expression is downregulated or soluble recombinant FGFR2b competes out the endogenous growth factors. However, a combination of neutralizing antibodies to FGF1, FGF7 and FGF10 is required to inhibit branching in the intact gland, suggesting that multiple FGF isoforms are required for branching. Exogenous FGFs added to submandibular epithelial rudiments cultured without mesenchyme induce distinct morphologies. FGF7 induces epithelial budding, whereas FGF10 induces duct elongation, and both are inhibited by FGFR or ERK1/2 signaling inhibitors. However, a PI3-kinase inhibitor also decreases FGF7-mediated epithelial budding, suggesting that multiple signaling pathways exist. We immunolocalized FGF receptors and analyzed changes in FGFR, FGF and MMP gene expression to identify the mechanisms of FGF-mediated morphogenesis. FGFR1b and FGFR2b are present throughout the epithelium,although FGFR1b is more highly expressed around the periphery of the buds and the duct tips. FGF7 signaling increases FGFR1b and FGF1expression, and MMP2 activity, when compared with FGF10, resulting in increased cell proliferation and expansion of the epithelial bud, whereas FGF10 stimulates localized proliferation at the tip of the duct. FGF7- and FGF10-mediated morphogenesis is inhibited by an MMP inhibitor and a neutralizing antibody to FGF1, suggesting that both FGF1 and MMPs are essential downstream mediators of epithelial morphogenesis. Taken together,our data suggests that FGFR2b signaling involves a regulatory network of FGFR1b/FGF1/MMP2 expression that mediates budding and duct elongation during branching morphogenesis.

show abstract

Polymer Hydrogels to Guide Organotypic and Organoid Cultures

Magno

Meinhardt

Werner

2020

Adv Funct Materials

View full text Add to dashboard Cite

Human organotypic and organoid cultures provide increasingly lifelike models of tissue/organ development and disease, enable more realistic drug screening, and may ultimately pave the way for new therapies. A broad variety of extracellular matrix-based or inspired materials is instrumental in these approaches. In this review article, the foundations of the related materials design are summarized with an emphasis on the advantages and limitations of decellularized and reconstituted biopolymeric matrices as well as biohybrid and fully synthetic polymer hydrogel systems applied to enable specific organotypic and organoid cultures. Recent progress in the fabrication of defined hydrogel systems offering thoroughly tunable biochemical and biophysical properties is highlighted. Potentialities of hydrogel-based approaches to address the persisting challenges of organoid technologies, namely scalability, connectivity/integration, reproducibility, parallelization, and in situ monitoring are discussed.

show abstract

EGF-dependent lobule formation and FGF7-dependent stalk elongation in branching morphogenesis of mouse salivary epithelium in vitro

Cited by 71 publications

References 26 publications

Lysophosphatidic acid cooperates with EGF in inducing branching morphogenesis of embryonic mouse salivary epithelium

Lysophosphatidic acid cooperates with EGF in inducing branching morphogenesis of embryonic mouse salivary epithelium

FGFR2b signaling regulates ex vivo submandibular gland epithelial cell proliferation and branching morphogenesis

Polymer Hydrogels to Guide Organotypic and Organoid Cultures

Contact Info

Product

Resources

About