EGF containing gelatin-based wound dressings

Ulubayram, Kezban

doi:10.1016/s0142-9612(00)00287-8

Cited by 283 publications

(168 citation statements)

References 36 publications

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“…In this view, the use of biocompatible, absorbable polymers such as pectin (Ninan et al, 2014), chitin and its derivative chitosan (Muzzarelli, 2009), gelatin (Ulubayram et al, 2001), polycaprolactone (Bui et al, 2014), hyaluronic acid (Estes et al, 1993), and others have been described.…”

Section: Figure 1 Wound Classification (Kyriacos Et Al 2008)mentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

441

215

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Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

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Section: Figure 1 Wound Classification (Kyriacos Et Al 2008)mentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

441

215

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“…There are generally two types of gelatin: Type A and Type B. Gelatin Type A is extracted and processed by acidic pretreatment of collagen, whereas gelatin Type B is obtained by alkaline pretreatment. The alkaline pretreatment converts glutamine and asparagine residues into glutamic and aspartic acids, respectively, which leads to higher carboxylic acid content for gelatin Type B than for gelatin Type A. Gelatin has several potential advantages over other natural proteins, such as its biological origin, biodegradability and commercial availability at low cost [24][25][26][27][28][29][30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

et al. 2008

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Electrospinning using natural proteins or synthetic polymers is a promising technique for the fabrication of fibrous scaffolds for various tissue engineering applications. However, one limitation of scaffolds electrospun from natural proteins is the need to cross-link with glutaraldehyde for stability, which has been postulated to lead to many complications in vivo including graft failure. In this study, we determined the characteristics of hybrid scaffolds composed of natural proteins including collagen and elastin, as well as gelatin, and the synthetic polymer poly(ε-caprolactone) (PCL), so to avoid chemical cross-linking. Fiber size increased proportionally with increasing protein and polymer concentrations, whereas pore size decreased. Electrospun gelatin/PCL scaffolds showed a higher tensile strength when compared to collagen/elastin/PCL constructs. To determine the effects of pore size on cell attachment and migration, both hybrid scaffolds were seeded with adipose-derived stem cells. Scanning electron microscopy and nuclei staining of cell-seeded scaffolds demonstrated complete cell attachment to the surfaces of both hybrid scaffolds, although cell migration into the scaffold was predominantly seen in the gelatin/PCL hybrid. The combination of natural proteins and synthetic polymers to create electrospun fibrous structures resulted in scaffolds with favorable mechanical and biological properties.

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“…The wounds were excised in 5 µm thick layers under the fascia, including a 5 mm margin of unwounded skin, bisected and fixed in 10% formalin for 24 hours. The samples were processed by H&E staining for morphological observations (Li, et al 2008;Ulubayram, et al 2001). …”

Section: Histological Analysismentioning

confidence: 99%

“…A pilot study carried out in 20 diabetic patients demonstrated Herberprot ® as a feasible and safe treatment to promote the healing of chronic wounds in patients with full thickness ulcers (Fernandez-Montequin, et al 2009). However, rhEGF´s short half life requires a continuous exposure (at least 6-12 hours) to enhance the mitogenic effect on epithelial (Hardwicke, et al 2008;Ulubayram, et al 2001). Therefore, in order to achieve a significant therapeutic effect, it is necessary to optimise the administration of growth factors such as rhEGF, in terms of dose, delivery system and safety.…”

Section: Introductionmentioning

confidence: 99%

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

Gainza

Aguirre

Pedraz

et al. 2013

European Journal of Pharmaceutical Sciences

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Diabetic foot ulcers (DFUs) represent a major clinical challenge in the ageing population. To address this problem, rhEGF-loaded Poly-Lactic-co-Glycolic-Acid (PLGA)-Alginate microspheres (MS) were prepared by a modified w/o/w-doubleemulsion/solvent evaporation method. Different formulations were evaluated with the aim of optimising MSs properties by adding NaCl to the surfactant solution and/or the solvent removal phase and adding alginate as a second polymer. The characterization of the developed MS showed that alginate incorporation increased the encapsulation efficiency (EE) and NaCl besides increasing the EE also became the particle surface smooth and regular. Once the MS were optimised, the target loading of rhEGF was increased to 1% (PLGA-Alginate MS), and particles were sterilised by gamma radiation to provide the correct dosage for in vivo studies. In vitro cell culture assays demonstrated that neither the microencapsulation nor the sterilisation process affected rhEGF bioactivity or rhEGF wound contraction. Finally, the MS were evaluated in vivo for treatment of the full-thickness wound model in diabetised Wistar rats. rhEGF MS treated animals showed a statistically significant decrease of the wound area by days 7 and 11, a complete re-epithelisation by day 11 and an earlier resolution of the inflammatory process. Overall, these findings demonstrate the promising potential of rhEGF-loaded MS (PLGA-Alginate MS) to promote faster and more effective wound healing, and suggest its possible application in DFU treatment.

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EGF containing gelatin-based wound dressings

Cited by 283 publications

References 36 publications

Chitosan as a starting material for wound healing applications

Chitosan as a starting material for wound healing applications

Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering

rhEGF-loaded PLGA-Alginate microspheres enhance the healing of full-thickness excisional wounds in diabetised Wistar rats

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