EGF and FGF-2 Infusion Increases Post-Ischemic Neural Progenitor Cell Proliferation in the Adult Rat Brain

Türeyen, Kudret; Vemuganti, Raghu; Bowen, Kellie K.; Sailor, Kurt A.; Dempsey, Robert J.

doi:10.1227/01.neu.0000186040.96929.8a

Cited by 91 publications

(69 citation statements)

References 51 publications

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“…Evidence supporting the importance of FGF2 in the maintenance of NSC proliferation has been recapitulated in multiple studies. 118,127 Similar findings have confirmed epidermal growth factor as a mitogen responsible for the maintenance of NSC proliferative capacity. 82,93 Physical dissociation of the desired tissue substrate is followed by isolation of the desired cell type.…”

Section: Neurosurg Focus / Volume 24 / March/april 2008supporting

confidence: 67%

Shifting the balance: cell-based therapeutics as modifiers of the amyotrophic lateral sclerosis–specific neuronal microenvironment

Riley

Sweeney

Boulis

2008

FOC

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✓ Recent advances in the laboratory have improved the current understanding of neurobiological mechanisms underlying the initiating events and pathological progression observed in amyotrophic lateral sclerosis (ALS). Whereas initial studies have revealed the late-stage intracellular cascades contributing to neuronal dysfunction and cell death, more recently collected data have begun to elucidate the presence and importance of a “non–cell autonomous” component indicating that affected glial cell subtypes may serve distinct and required roles. Pharmacological interventions for ALS have largely been disappointing likely in part because they have failed to address either the proximate events contributing to neuronal dysfunction and death or the deleterious contributions of non-neuronal cells within the local microenvironment. Alternatively, cell-based therapeutics offer the potential of a multifaceted approach oriented toward the dual ends of protecting remaining viable neurons and attempting to restore neuronal function lost as a manifestation of disease progression. The authors review the evolving knowledge of disease initiation and progression, with specific emphasis on the role of affected glia as crucial contributors to the observed ALS phenotype. This basis is used to underscore the potential roles of cell-based therapeutics as modifiers of the ALS-specific microenvironment.

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Section: Neurosurg Focus / Volume 24 / March/april 2008supporting

confidence: 67%

Shifting the balance: cell-based therapeutics as modifiers of the amyotrophic lateral sclerosis–specific neuronal microenvironment

Riley

Sweeney

Boulis

2008

FOC

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“…Despite the exciting prospects of these cells, considerable safety concerns remain regarding their potential to form teratomas. (For a more detailed review of [81] Teramoto et al, 2003 [71] Cooke et al, 2011 [135] Türeyen et al, 2005 [153] Sugiura et al, 2005 [127] Jin et al, 2004 [74] Sun et al, 2003 [154] Wang et al, 2009 [128] Zhu et al, 2008 [155] , 2009 [83] Popa-Wagner et al, 2010 [80] Kobayashi et al, 2006 [78] Guerra-Crespo et al, 2009 [85] Chou et al, 2006 [84] Wang et al, 2012 [113] Schäbitz et al, 2007 [34] Li et al, 2011 [94] Chern et al, 2012 [93] Kilic et al, 2008 [92] Wang et al, 2004 [87] Wang et al, 2012 [89] www.chinaphar.com Dibajnia P et al Acta Pharmacologica Sinica npg different stem cells, see Burns and Steinberg [43] ). Transplantation of NPCs has advantages over other cell types for a number of reasons.…”

Section: Stroke Models and Outcome Measuresmentioning

confidence: 99%

Role of neural precursor cells in promoting repair following stroke

Dibajnia

Morshead

2012

Acta Pharmacol Sin

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Stem cell-based therapies for the treatment of stroke have received considerable attention. Two broad approaches to stem cell-based therapies have been taken: the transplantation of exogenous stem cells, and the activation of endogenous neural stem and progenitor cells (together termed neural precursors). Studies examining the transplantation of exogenous cells have demonstrated that neural stem and progenitor cells lead to the most clinically promising results. Endogenous activation of neural precursors has also been explored based on the fact that resident precursor cells have the inherent capacity to proliferate, migrate and differentiate into mature neurons in the uninjured adult brain. Studies have revealed that these neural precursor cell behaviours can be activated following stroke, whereby neural precursors will expand in number, migrate to the infarct site and differentiate into neurons. However, this innate response is insufficient to lead to functional recovery, making it necessary to enhance the activation of endogenous precursors to promote tissue repair and functional recovery. Herein we will discuss the current state of the stem cell-based approaches with a focus on endogenous repair to treat the stroke injured brain.

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“…23 Neural progenitors express receptors for basic fibroblast growth factor. 24 The prosurvival effect of brain lesions has been reported. 25 However, it was no longer significant after differentiation into NPs, which apparently differ from early NPG in their sensitivity to extrinsic trophic factors or to transplantation.…”

Section: Seminatore Et Al Impact Of Ischemia On Hesc-neural Progenitorsmentioning

confidence: 99%

The Postischemic Environment Differentially Impacts Teratoma or Tumor Formation After Transplantation of Human Embryonic Stem Cell-Derived Neural Progenitors

Seminatore

Polentes²,

Ellman³

et al. 2010

Stroke

122

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Background and Purpose-Risk of tumorigenesis is a major obstacle to human embryonic and induced pluripotent stem cell therapy. Likely linked to the stage of differentiation of the cells at the time of implantation, formation of teratoma/tumors can also be influenced by factors released by the host tissue. We have analyzed the relative effects of the stage of differentiation and the postischemic environment on the formation of adverse structures by transplanted human embryonic stem cell-derived neural progenitors. Methods-Four differentiation stages were identified on the basis of quantitative polymerase chain reaction expression of pluripotency, proliferation, and differentiation markers. Neural progenitors were transplanted at these 4 stages into rats with no, small, or large middle cerebral artery occlusion lesions. The fate of each transplant was compared with their pretransplantation status 1 to 4 months posttransplantation. Results-The influence of the postischemic environment was limited to graft survival and occurrence of nonneuroectodermal structures after transplantation of very immature neural progenitors. Both effects were lost with differentiation. We identified a particular stage of differentiation characterized in vitro by a rebound of proliferative activity that produced highly proliferative grafts susceptible to threaten surrounding host tissues. Conclusion-The effects of the ischemic environment on the formation of teratoma by transplanted human embryonic stem cell-derived neural progenitors are limited to early differentiation stages that will likely not be used for stem cell therapy. In contrast, hyperproliferation observed at later stages of differentiation corresponds to an intrinsic activity that should be monitored to avoid tumorigenesis. (Stroke. 2010;41:153-159.)

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EGF and FGF-2 Infusion Increases Post-Ischemic Neural Progenitor Cell Proliferation in the Adult Rat Brain

Abstract: Specific growth factor infusion enhances post-ischemic progenitor cell proliferation by 5 days of reperfusion and neuronal maturation by 21 days of reperfusion in both the DG and SVZ in the adult rat brain.

Cited by 91 publications

References 51 publications

Shifting the balance: cell-based therapeutics as modifiers of the amyotrophic lateral sclerosis–specific neuronal microenvironment

Shifting the balance: cell-based therapeutics as modifiers of the amyotrophic lateral sclerosis–specific neuronal microenvironment

Role of neural precursor cells in promoting repair following stroke

The Postischemic Environment Differentially Impacts Teratoma or Tumor Formation After Transplantation of Human Embryonic Stem Cell-Derived Neural Progenitors

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