EGDMA- and TRIM-Based Microparticles Imprinted with 5-Fluorouracil for Prolonged Drug Delivery

Abstract

Imprinted materials possess designed cavities capable of forming selective interactions with molecules used in the imprinting process. In this work, we report the synthesis of 5-fluorouracil (5-FU)-imprinted microparticles and their application in prolonged drug delivery. The materials were synthesized using either ethylene glycol dimethacrylate (EGDMA) or trimethylolpropane trimethacrylate (TRIM) cross-linkers. For both types of polymers, methacrylic acid was used as a functional monomer, whereas 2-hydroxyethyl methacrylate was applied to increase the final materials’ hydrophilicity. Adsorption isotherms and adsorption kinetics were investigated to characterize the interactions that occur between the materials and 5-FU. The microparticles synthesized using the TRIM cross-linker showed higher adsorption properties towards 5-FU than those with EGDMA. The release kinetics was highly dependent upon the cross-linker and pH of the release medium. The highest cumulative release was obtained for TRIM-based microparticles at pH 7.4. The IC50 values proved that 5-FU-loaded TRIM-based microparticles possess cytotoxic activity against HeLa cell lines similar to pure 5-FU, whereas their toxicity towards normal HDF cell lines was ca. three times lower than for 5-FU.

Study of Hydroxypropyl β-Cyclodextrin and Puerarin Inclusion Complexes Encapsulated in Sodium Alginate-Grafted 2-Acrylamido-2-Methyl-1-Propane Sulfonic Acid Hydrogels for Oral Controlled Drug Delivery

“…The bands at 1360 cm −1 are attributed to asymmetric stretching of the S=O group, while 1230 cm −1 represents symmetric stretching of the S=O group [ 23 ]. The FTIR spectra of EGDMA showed a vibration band at 1713 cm −1 , which corresponds to the stretching vibrations of C=O, and bands at 1633, 1291, and 1153 cm −1 are ascribed to the C=C and C–O stretching vibrations of ester groups [ 24 , 25 ]. The FTIR spectrum of pure HP-βCD demonstrated the stretching vibration of –OH at 3410 cm −1 and a symmetric stretching vibration of C-H at 2928 cm −1 .…”

Application of Molecular Imprinting for Creation of Highly Selective Sorbents for Extraction and Separation of Rare-Earth Elements

“…At the end of the process, template molecules are removed from the cross-linked polymer matrix. As a result, “imprints” (the so-called “imprint sites”) are formed in the polymer, which are cavities that are complementary to the template molecule in size, shape and arrangement of functional groups [ 47 ]. Briefly, the process of pore formation in MIPs can be described as follows.…”

Attapulgite and Graphene‐Based Molecular Imprinted Polymers as 5‐FU Delivery Systems for the Treatment of Cervical Cancer**

“…Compared to other 5-FU MIPs, ATP-and graphene-based imprinted polymers were able to enhance the interaction between 5-FU and the polymers and prolong the in vitro administration of 5-FU. [19][20][21][22][23] Thus, the aim of this manuscript is to introduce a surface molecularly imprinted polymer synthesized with ATP@GO matrix used as a controlled-release carrier for 5-FU. MIPs were systematically characterized by X-ray diffraction analysis (XRD), field emission scanning electron microscopy (FESEM), highresolution transmission microscopy (HRTEM), Fourier transform infrared spectroscopy (FTIR), and thermogravimetry (TG).…”

“…The special pore structure of ATP and the interaction between graphene and aromatic drugs were combined to enhance the MIPs′ ability to control the release of 5‐FU. Compared to other 5‐FU MIPs, ATP‐ and graphene‐based imprinted polymers were able to enhance the interaction between 5‐FU and the polymers and prolong the in vitro administration of 5‐FU [19–23] …”