EFICÁCIA IN VITRO DO ÓLEO ESSENCIAL DE CAPIM-LIMÃO (Cymbopogon flexuosus STEUD. WATS.) FRENTE A BACTÉRIAS ENTÉRICAS DE ORIGEM AVÍCOLA

Abstract

O uso de antimicrobianos como melhoradores de desempenho tem representado um risco à saúde pública devido à associação destes com o surgimento de resistência bacteriana e resíduos nos produtos. Como alternativa, pesquisas têm indicado a utilização de aditivos alimentares naturais, como o óleo essencial de capimlimão. Objetivou-se neste artigo avaliar a atividade antimicrobiana do óleo essencial de capim-limão (Cymbopogon flexuosus) frente a Staphylococcus aureus e Escherichia coli entéricas isoladas de aves de postura (Gallus gallus domesticus). Foram preparadas cinco concentrações do óleo essencial (160; 80; 40; 20 e 10 µL mL-1) e então realizados testes in vitro de difusão em disco e determinação da Concentração Inibitória Mínima (CIM) e da Concentração Bactericida Mínima (CBM). Os resultados indicaram que a concentração de 160 µL mL-1 propiciou os maiores diâmetros de halos de inibição na difusão em disco, diferindo-se estatisticamente das demais concentrações (p < 0,05). Houve efeito inibitório (CIM) a partir da concentração de 80 µL mL-1 de óleo para todas as bactérias mas a CBM não foi detectada. Conclui-se que o óleo essencial de capim-limão apresenta atividade antimicrobiana in vitro frente a S. aureus e E. coli isoladas de aves na concentração de 160 µL mL-1. Novos estudos são necessários para avaliar a eficácia do uso de doses superiores e indicar esse óleo como aditivo em dietas de aves.

Disinfection of Fertile Eggs of Free-Range Poultry with Essential Oils

“…Despite the conditions of the experiment, the antimicrobial activity was evidenced by the significant reduction in total microorganism counts. The antimicrobial activity of essential oils from C. flexuosus and L. rotundifolia have been confirmed by Adukwu et al (2012), Millezi et al (2012), Azevedo et al (2016), andAssis et al (2017). Copur et al (2010) evaluated essential oil from O. vulgare and found a disinfecting effect on broiler eggs, with reduction of total bacteria, and fungi and yeasts when performing fumigation of this essential oil at concentrations of 0.55 and 0.75 µL cm -3 , compared to the control and to the use of formaldehyde; they emphasized the potential of this natural disinfectant for hatcheries.…”

“…Both essential oils were extracted by steam distillation. According to the analysis of gas chromatography coupled to mass spectrometry performed by Azevedo et al (2016), the main component of the essential oil from C. flexuosus is citral (77.42%),and a mixture of neral (β-Citral) and geranial (α-Citral) isomers; and the main component of the essential oil from L. rotundifolia is β-myrcene (15.52%). Eggs from free-range poultry of unknown breed collected in a noncommercial farm were incubated.…”

“…The interest in using essential oils has been increasing due to their antimicrobial properties, and because they are accepted by biosafety programs. Antimicrobial activity of essential oils has been reported by Adukwu et al (2012), Sinha et al (2014), Souza et al (2014), Azevedo et al (2016), andAssis et al (2017). In this context, the objective of the present study was to evaluate the effect of the disinfection of fertile eggs of free-range poultry with essential oils from Cymbopogon Flexuosus and Lippia Rotundifolia.…”

Use of Lippia rotundifolia and Cymbopogon flexuosus essential oils, individually or in combination, in broiler diets

“…The treatments were: negative control, without antimicrobials and anticoccidials; positive control -feed supplemented with 10 ppm enramycin and 42 ppm salinomycin; lemongrass -feed containing 120 mg of lemongrass essential oil for each kg -1 of animal body weight; L. rotundifolia -feed containing 120 mg of L. rotundifolia essential oil for each kg -1 of animal body weight; combination -feed with the mixture of the two essential oils. The dose used was defined from the antimicrobial activity presented by the essential oils of L. rotundifolia (Souza et al, 2015) and lemongrass (Azevedo et al, 2016) in preliminary in vitro tests.…”

Antioxidant activity of essential oils from condiment plants and their effect on lactic cultures and pathogenic bacteria

“…NAVEED et al 2013 antibacterial activity of C. citratus oil and evidenced a clear growth-inhibition zone (>18mm) formed by E. coli, Staphylococcus aureus and Salmonella tiphi. AZEVEDO et al (2016) adopted this methodology to address the antimicrobial activity of the essential oil from Cymbopogon flexuosus against enteric S. aureus and E. coli isolated from laying hens. Based on their results, the concentration 160μL mL -1 recorded the largest inhibition zone diameters for all bacteria during disk diffusion.…”