Efficient vanillin biosynthesis by recombinant lignin-degrading bacterium Arthrobacter sp. C2 and its environmental profile via life cycle assessment

Zhao, Xinyue; Zhang, Yuting; Jiang, Hanyi; Zang, Hailian; Wang, Yue; Sun, Shanshan; Li, Chunyan

doi:10.1016/j.biortech.2021.126434

Cited by 23 publications

(3 citation statements)

References 47 publications

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“…1.6.5.6), which are involved in the metabolism of quinone, an intermediate metabolite of aromatic compounds that are commonly found in the plant kingdom especially in higher plants [ 55 , 56 ]. AA4 members are exclusively vanillyl-alcohol oxidases (EC 1.1.3.38), and it has been reported that they can oxidize vanillyl alcohol as a lignin unit to vanillin and further metabolize it [ 57 ]. It is likely that these enzymes require the conditions of AA1 and AA2 to function, which may explain their reduced abundance at the phylum level after fermentation.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Fermentation of Beechwood Lignin–Carbohydrate Complexes Provides Evidence for Utilization by Gut Bacteria

Liu

Wang

et al. 2023

Nutrients

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Lignin–carbohydrate complexes (LCCs) are emerging as a new and natural product with pharmacological and nutraceutical potential. It is uncertain, however, whether LCCs have a positive effect on the microbiota of the gut based on the current evidence. Here, the LCC extracted from beechwood (BW-LCC) was used as a substrate for in vitro fermentation. The lignin in BW-LCC consisted of guaiacyl (G) and syringyl (S) units, which are mainly linked by β-O-4 bonds. After 24 h of in vitro fermentation, the pH had evidently declined. The concentrations of acetic acid and propionic acid, the two main short-chain fatty acids (SCFAs), were significantly higher than in the control group (CK). In addition, BW-LCC altered the microbial diversity and composition of gut microbes, including a reduction in the relative abundance of Firmicutes and an increase in the relative abundance of Proteobacteria and Bacteroidetes. The relative abundance of Escherichia coli-Shigella and Bacteroides were the most variable at the genus level. The genes of carbohydrate-active enzymes (CAZymes) also changed significantly with the fermentation and were related to the changes in microbes. Notably, the auxiliary actives (AAs), especially AA1, AA2, and AA3_2, play important roles in lignin degradation and were significantly enriched and concentrated in Proteobacteria. From this study, we are able to provide new perspectives on how gut microbes utilize LCC.

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Section: Resultsmentioning

confidence: 99%

In Vitro Fermentation of Beechwood Lignin–Carbohydrate Complexes Provides Evidence for Utilization by Gut Bacteria

Liu

Wang

et al. 2023

Nutrients

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“…In this study, openLCA software, coupled with the Ecoinvent v3.4 database, , was used to convert the inventory data into assessment results to assess the environmental impacts. The characteristic impacts of the eight processes were obtained according to the ReCiPe Midpoint and End point method …”

Section: Methodsmentioning

confidence: 99%

Biochar and Hydrochar from Agricultural Residues for Soil Conditioning: Life Cycle Assessment and Microbially Mediated C and N Cycles

Wang

Shao

et al. 2022

ACS Sustainable Chem. Eng.

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Value-added biochar or hydrochar converted from agricultural waste has the potential to improve soil performance. However, how biochar and hydrochar addition affect ecological responses is not clear. Here, we examined the physicochemical property of biochar and hydrochar from straw (RS) and manure (SM), identified relevant eco-friendly pretreatment methods through life cycle assessment (LCA) methodology, and investigated their influences on soil properties and C and N cycles. The results showed that biochar prepared at 500 and 700 °C and hydrochar prepared at 250 °C for 4, 6, and 8 h had better physicochemical properties. The LCA results revealed that RBC500, RS250-4, and SM250-4 had lower environmental burdens in the preparation process. Moreover, biochar and hydrochar addition could promote the soil C and N cycles to varying degrees, especially in the case of SM250-4, which could simultaneously promote the degradation of cellulose, lignin, and chitin and reduce the emission of CO2 and N2O. Overall, SM250-4 application had a beneficial effect on carbon neutralization. These novel results compensate for the deficiency of research on the phaeozem improvement effects of biochar and hydrochar.

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“…Biological funneling for the conversion of lignin-related aromatic compound (LRC) mixtures to performance-advantaged bioproducts (13,14) has been demonstrated, and some of the associated bioproducts include 2-pyrone-4,6-dicarboxylic acid (15)(16)(17), itaconic acid (18), polyhydroxyalkanoates (8,19,20), cis,cis-muconic acid (21)(22)(23)(24), vanillin (25,26), substituted styrene molecules (27), pyridine-2,4-dicarboxylic acids (28,29), and β-ketoadipic acid (30), among others, reviewed recently by Weiland et al (12). β-Ketoadipic acid is a six-carbon dicarboxylic acid with a β-ketone (Fig.…”

Section: Introductionmentioning

confidence: 99%

Lignin conversion to β-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development

Werner,

Cordell,

Lahive

et al. 2023

Sci. Adv.

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Bioconversion of a heterogeneous mixture of lignin-related aromatic compounds (LRCs) to a single product via microbial biocatalysts is a promising approach to valorize lignin. Here, Pseudomonas putida KT2440 was engineered to convert mixed p-coumaroyl– and coniferyl-type LRCs to β-ketoadipic acid, a precursor for performance-advantaged polymers. Expression of enzymes mediating aromatic O -demethylation, hydroxylation, and ring-opening steps was tuned, and a global regulator was deleted. β-ketoadipate titers of 44.5 and 25 grams per liter and productivities of 1.15 and 0.66 grams per liter per hour were achieved from model LRCs and corn stover-derived LRCs, respectively, the latter representing an overall yield of 0.10 grams per gram corn stover-derived lignin. Technoeconomic analysis of the bioprocess and downstream processing predicted a β-ketoadipate minimum selling price of $2.01 per kilogram, which is cost competitive with fossil carbon-derived adipic acid ($1.10 to 1.80 per kilogram). Overall, this work achieved bioproduction metrics with economic relevance for conversion of lignin-derived streams into a performance-advantaged bioproduct.

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Efficient vanillin biosynthesis by recombinant lignin-degrading bacterium Arthrobacter sp. C2 and its environmental profile via life cycle assessment

Cited by 23 publications

References 47 publications

In Vitro Fermentation of Beechwood Lignin–Carbohydrate Complexes Provides Evidence for Utilization by Gut Bacteria

In Vitro Fermentation of Beechwood Lignin–Carbohydrate Complexes Provides Evidence for Utilization by Gut Bacteria

Biochar and Hydrochar from Agricultural Residues for Soil Conditioning: Life Cycle Assessment and Microbially Mediated C and N Cycles

Lignin conversion to β-ketoadipic acid by Pseudomonas putida via metabolic engineering and bioprocess development

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