Efficient Utilization of Lignocellulosic Biomass: Hydrolysis Methods for Biorefineries

Aich, Shritama; Datta, Supratim

doi:10.1007/978-981-15-9593-6_11

Cited by 5 publications

(5 citation statements)

References 81 publications

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“… 13 Cellulose, a readily available material, can be hydrolyzed into glucose by cellulases and further processed to produce biofuels. 14 The major components of cellulase are endoglucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (BGL). However, the multi-enzyme catalyzed hydrolysis of carboxymethylcellulose (CMC), a modified form of cellulose, to glucose with high synthetic efficiency is challenging.…”

Section: Resultsmentioning

confidence: 99%

Hierarchical covalent organic framework-foam for multi-enzyme tandem catalysis

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Hierarchical covalent organic framework-foam for multi-enzyme tandem catalysis

et al. 2023

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“…Cellulolytic enzymes BGL, CBH, and EG were used for purification and immobilization. Cellulolytic enzymes can break down cellulose into glucose, which can then be processed further to produce biofuels . The BGL@Ni-TpBpy COF was employed to catalyze the hydrolysis of p NPGlc to p NP.…”

Section: Resultsmentioning

confidence: 99%

Covalent Organic Frameworks for the Purification of Recombinant Enzymes and Heterogeneous Biocatalysis

Paul,

Gupta,

Kumar Mahato

et al. 2023

J. Am. Chem. Soc.

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Recombinant enzymes have gained prominence due to their diverse functionalities and specificity and are often a greener alternative in biocatalysis. This context makes purifying recombinant enzymes from host cells and other impurities crucial. The primary goal is to isolate the pure enzyme of interest and ensure its stability under ambient conditions. Covalent organic frameworks (COFs), renowned for their well-ordered structure and permeability, offer a promising approach for purifying histidinetagged (His-tagged) enzymes. Furthermore, immobilizing enzymes within COFs represents a growing field in heterogeneous biocatalysis. In this study, we have developed a flow-based technology utilizing a nickel-infused covalent organic framework (Ni-TpBpy COF) to combine two distinct processes: the purification of His-tagged enzymes and the immobilization of enzymes simultaneously. Our work primarily focuses on the purification of three Histagged enzymes β-glucosidase, cellobiohydrolase, and endoglucanase as well as two proteins with varying molecular weights, namely, green fluorescent protein (27 kDa) and BG Rho (88 kDa). We employed Ni-TpBpy as a column matrix to showcase the versatility of our system. Additionally, we successfully obtained a Ni-TpBpy COF immobilized with enzymes, which can serve as a heterogeneous catalyst for the hydrolysis of p-nitrophenyl-β-D-glucopyranoside and carboxymethylcellulose. These immobilized enzymes demonstrated catalytic activity comparable to that of their free counterparts, with the added advantages of recyclability and enhanced stability under ambient conditions for an extended period, ranging from 60 to 90 days. This contrasts with the free enzymes, which do not maintain their activity as effectively over time.

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“…The hydrolysis of lignocellulosic biomass by cellulase promises to be a sustainable method for producing biofuels and an alternative to fossil fuels. The shift in energy sources can reduce the adverse effects of fossil fuel use on the environment, particularly greenhouse gas emissions, and benefit developing countries by lowering import costs of fossil fuels. , The cellulase cocktail hydrolyzes the cellulose in lignocellulosic biomass, producing glucose; this constitutes a crucial step in biofuel production from plant biomass. The cellulase cocktail is composed of at least three enzymes, including endoglucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (BG) .…”

Section: Introductionmentioning

confidence: 99%

Thermal Sensitivity of the Enzymatic Activity of β-Glucosidase: Simulations Lend Mechanistic Insights into Experimental Observations

Sahu,

Ghosh,

Sinha

et al. 2023

Biochemistry

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Efficient Utilization of Lignocellulosic Biomass: Hydrolysis Methods for Biorefineries

Cited by 5 publications

References 81 publications

Hierarchical covalent organic framework-foam for multi-enzyme tandem catalysis

Hierarchical covalent organic framework-foam for multi-enzyme tandem catalysis

Covalent Organic Frameworks for the Purification of Recombinant Enzymes and Heterogeneous Biocatalysis

Thermal Sensitivity of the Enzymatic Activity of β-Glucosidase: Simulations Lend Mechanistic Insights into Experimental Observations

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