Efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast

Abstract

Miniature inverted repeat transposable elements (MITEs) are often the most numerous DNA transposons in plant and animal genomes. The dramatic amplification of MITE families during evolution is puzzling, because the transposase sources for the vast majority of MITE families are unknown. The yellow fever mosquito genome contains > 220-Mb MITE sequences; however, transposition activity has not been demonstrated for any of the MITE families. The Gnome elements are the youngest MITE family in this genome, with at least 116 identical copies. To test whether the putative autonomous element Ozma is capable of mobilizing Gnome and its two sibling MITEs, analyses were performed in a yeast transposition assay system. Whereas the wild-type transposase resulted in very low transposition activity, mutations in the region containing a putative nuclear export signal motif resulted in a dramatic (at least 4160-fold) increase in transposition frequency. We have also demonstrated that each residue of the novel DD37E motif is required for the activity of the Ozma transposase. Footprint sequences left at the donor sites suggest that the transposase may cleave between the second and the third nucleotides from the 5' ends of the elements. The excised elements reinsert specifically at dinucleotide 'TA',~55% of them in yeast genes. The elements described in this article could potentially be useful as genetic tools for genetic manipulation of mosquitoes.

Nuclear export signal (NES) of transposases affects the transposition activity of mariner-like elements Ppmar1 and Ppmar2 of moso bamboo

“…The mutant of Ping ( mPing ) had an increased activity by about six times when the Ping TPase-NES was weakened by mutation (L384/386A), while the Pong mutant had an increased excision and transposition activity by about 16 times following an NES mutation (L418/420A) [24]. In the same way, the NES mutant of Ozma TPase had resulted in an increased transposition activity of at least 4160 times for the Eif and Goblin MITEs in yeast [19].…”

“…Hancock et al [24] found that a mutation of the NES domain in the TPase of mPing , a deletion derivative of autonomous rice PIF/Harbinger transposon Ping , increased transposition activity in both yeast and plants. Similarly, Fattash et al [19] identified that a mutation of a putative NES in the Ozma TPase dramatically increased transposition of the Eif and Goblin MITEs in yeast.…”

“…Structurally, they contain a DNA binding domain and a catalytic domain [17]. Additionally, the TPase contains one or more short sequences of nuclear localization signals (NLS) [18] and nuclear export signals (NES) [19]. NES also is a short (8–15 residues) amino acid sequence consisting usually of four to five hydrophobic residues in a protein [20].…”

Influência de elementos genéticos móveis em linhagens de <i>Xanthomonas oryzae</i>.

“…A dissonância observada na cepa africana representa mais uma evidência de uma história evolutiva de AXO1947 distinta das cepas asiáticas, que pode ser atribuída a recente pressão de seleção para adaptação de X. oryzae ao cultivares de arroz nesta região (Gonzalez et al, 2007;Soto-Suárez et al, 2010). (Gonzalez et al, 2007;McDonald, Linde, 2002;Wonni, 2016 (Philippe et al, 2007;Vital et al, 2015 (Fattash et al, 2015;Han et al, 2010;Hikosaka et al, 2011;Luchetti, 2015) e de plantas agronomicamente importantes (Lu et al, 2012;Nouroz et al, 2015;Oki et al, 2008;Vaschetto 2016;Yaakov et al, 2013 O software SeaView (Gouy et al, 2010) foi utilizado para gerar alinhamentos, utilizando o algoritmo MAFFT (Katoh et al, 2002). IQ-TREE (Nguyen et al, 2015)…”