Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene

Brown, Liana E.; Elliott, T. S. J.

doi:10.1128/jb.178.13.3763-3770.1996

Cited by 186 publications

(203 citation statements)

References 70 publications

(78 reference statements)

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“…Mutants lacking Hfq have been isolated in a number of bacterial species; although viable, these strains are debilitated and more susceptible than wild type to stress treatments. Such impairments have multiple causes including the inability of hfq mutants to fully induce the RpoS-dependent general stress response, due to the loss of sRNA-mediated translational activation of rpoS mRNA (Brown and Elliott 1996;Majdalani et al 1998), and a toxic overexpression of the RpoE regulon ascribable to loss of sRNA-mediated envelope homeostasis (Ding et al 2004;Figueroa-Bossi et al 2006;Guisbert et al 2007;Sittka et al 2007).…”

mentioning

confidence: 99%

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Figueroa‐Bossi¹,

Valentini²,

Malleret³

et al. 2009

Genes Dev.

206

254

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A relevant, yet little recognized feature of antisense regulation is the possibility of switching roles between regulatory and regulated RNAs. Here we show that induction of a Salmonella gene relies on the conversion of a small RNA from effector to regulatory target. The chiP gene (formerly ybfM), identified and characterized in the present study, encodes a conserved enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. In the absence of inducer, chiP is kept silent by the action of a constitutively made small RNA, ChiX (formerly SroB, RybC), which pairs with a sequence at the 59 end of chiP mRNA. Silencing is relieved in the presence of chitooligosaccharides due to the accumulation of an RNA that pairs with ChiX and promotes its degradation. Anti-ChiX RNA originates from an intercistronic region of the chb operon, which comprises genes for chitooligosaccharide metabolism and whose transcription is activated in the presence of these sugars. We present evidence suggesting that the chb RNA destabilizes ChiX sRNA by invading the stem of its transcription terminator hairpin. Overall, our findings blur the distinction between effector and target in sRNA regulation, raising the possibility that some of the currently defined targets could actually be inhibitors of sRNA function.[Keywords: Antisense regulation; chitoporin; chitobiose; Hfq; small RNA] Supplemental material is available at http://www.genesdev.org.

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mentioning

confidence: 99%

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Figueroa‐Bossi¹,

Valentini²,

Malleret³

et al. 2009

Genes Dev.

206

254

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“…5A). The pTZWF and pTX suicide plasmids containing a unique FRT site were integrated into the Salmonella chromosome in an intergenic region downstream of the putP gene (43). The constructs were transduced into the dksA mutant strain AV09294, generating AV10080 and AV10158, respectively.…”

Section: Redox Balance Associated With a Functional Dksa Supports Thementioning

confidence: 99%

Control of Redox Balance by the Stringent Response Regulatory Protein Promotes Antioxidant Defenses of Salmonella

Henard

Bourret

Song

et al. 2010

Journal of Biological Chemistry

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“…A well-studied example is the Hfq-dependent positive regulation of rpoS mRNA translation by the sRNAs DsrA, RprA and ArcZ in Escherichia coli and Salmonella typhimurium (Majdalani et al, 2002(Majdalani et al, , 2005Papenfort et al, 2009). Loss of hfq was shown to result in decreased rpoS transcript levels in E. coli (Muffler et al, 1996) and S. typhimurium (Brown & Elliott, 1996). The absence of Hfq and sRNA binding to the rpoS mRNA, which protects it from degradation by endonucleases (McCullen et al, 2010), results in enhanced degradation of the transcript.…”

Section: Introductionmentioning

confidence: 99%

The stationary phase sigma factor, RpoS, regulates the production of a carbapenem antibiotic, a bioactive prodigiosin and virulence in the enterobacterial pathogen Serratia sp. ATCC 39006

Wilf

Salmond

2012

Microbiology

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Serratia sp. ATCC 39006 (S39006) is a Gram-negative bacterium that is virulent in plant (potato) and invertebrate animal (Caenorhabditis elegans) models. It produces two secondary metabolite antibiotics, a prodigiosin and a carbapenem, and the exoenzymes pectate lyase and cellulase. We showed previously that deletion of the RNA chaperone Hfq abolished antibiotic production and attenuated virulence in both animal and plant hosts. Hfq and dependent small RNAs (sRNAs) are known to regulate the post-transcriptional expression of rpoS, which encodes s S , the stationary phase sigma factor subunit of RNA polymerase. An S39006 hfq deletion mutant showed decreased transcript levels of rpoS. Therefore, in this study we investigated whether the phenotypes regulated by Hfq were mediated through its control of rpoS. Whereas loss of Hfq abolished prodigiosin and carbapenem production and attenuated virulence in both C. elegans and potato, characterization of an S39006 rpoS mutant showed unexpectedly elevated prodigiosin and carbapenem production. Furthermore, the rpoS mutant exhibited attenuated animal pathogenesis, but not plant pathogenesis. Additionally, a homologue of the Hfq-dependent sRNA, RprA, was identified and shown to regulate prodigiosin production in a manner consistent with its role in positively regulating translation of rpoS mRNA. Combined, these results demonstrate that Hfq regulation of secondary metabolism and plant pathogenesis is independent of RpoS and establishes RpoS and RprA as regulators of antibiotic production.

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Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene

Cited by 186 publications

References 70 publications

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Caught at its own game: regulatory small RNA inactivated by an inducible transcript mimicking its target

Control of Redox Balance by the Stringent Response Regulatory Protein Promotes Antioxidant Defenses of Salmonella

The stationary phase sigma factor, RpoS, regulates the production of a carbapenem antibiotic, a bioactive prodigiosin and virulence in the enterobacterial pathogen Serratia sp. ATCC 39006

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