Efficient transformation and artificial mi<scp>RNA</scp> gene silencing in <i><scp>L</scp>emna minor</i>

Cantó-Pastor, Alex; Mollá‐Morales, Almudena; Ernst, Evan; Dahl, William; Zhai, Jixian; Yan, Yiheng; Meyers, Blake C.; Shanklin, John; Martienssen, Robert A.

doi:10.1111/plb.12215

Cited by 60 publications

(42 citation statements)

References 34 publications

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“…A truly tiny (2 cm) member, the fast-growing Lemna minor , has additionally been proposed as a model for aquatic plants, as a biofuel source, and because of its sensitivity to environmental toxins, for ecophysiological studies (Van Hoeck et al, 2015). The diploid genome is 472 Mb (Van Hoeck et al, 2015), and efficient genetic transformation and gene silencing via artificial microRNAs have been established (Cantó-Pastor et al, 2015). The smallest known monocot genome (158 Mb) belongs to another duckweed, Spirodela polyrhiza , whose genome sequence suggests that reductions in several biochemical pathways accompany its aquatic lifestyle and that its neotenous life cycle is reflected in changes in paralog numbers of promoters and repressors of the juvenile-to-adult transition (Wang et al, 2014).…”

Section: Bioenergy Cropsmentioning

confidence: 99%

Field Guide to Plant Model Systems

Chang

Bowman

Meyerowitz

2016

Cell

108

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For the past several decades, advances in plant development, physiology, cell biology, and genetics have relied heavily on the model (or reference) plant Arabidopsis thaliana. Arabidopsis resembles other plants, including crop plants, in many but by no means all respects. Study of Arabidopsis alone provides little information on the evolutionary history of plants, evolutionary differences between species, plants that survive in different environments, or plants that access nutrients and photosynthesize differently. Empowered by the availability of large-scale sequencing and new technologies for investigating gene function, many new plant models are being proposed and studied.

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Section: Bioenergy Cropsmentioning

confidence: 99%

Field Guide to Plant Model Systems

Chang

Bowman

Meyerowitz

2016

Cell

108

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“…Duckweed has been used in many ecotoxicological and phytoremediation studies and there is growing interest in duckweed as biofuel, animal feedstock, and food [14,15]. The recent development of duckweed genomic resources and molecular tools have positioned duckweed as a model system for several aspects of plant biology [16][17][18][19]. Duckweed has a simple body architecture consisting of mainly leaf-like structures termed fronds that float on the water surface while roots are only found in some species as simple roots with no lateral branching or root hairs [13].…”

Section: Introductionmentioning

confidence: 99%

Duckweed hosts a taxonomically similar bacterial assemblage as the terrestrial leaf microbiome

Acosta

Gilbert

et al. 2020

PLoS ONE

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Culture-independent characterization of microbial communities associated with popular plant model systems have increased our understanding of the plant microbiome. However, the integration of other model systems, such as duckweed, could facilitate our understanding of plant microbiota assembly and evolution. Duckweeds are floating aquatic plants with many characteristics, including small size and reduced plant architecture, that suggest their use as a facile model system for plant microbiome studies. Here, we investigated the structure and assembly of the duckweed bacterial microbiome. First, a culture-independent survey of the duckweed bacterial microbiome from different locations in New Jersey revealed similar phylogenetic profiles. These studies showed that Proteobacteria is a dominant phylum in the duckweed bacterial microbiome. To observe the assembly dynamics of the duckweed bacterial community, we inoculated quasi-gnotobiotic duckweed with wastewater effluent from a municipal wastewater treatment plant. Our results revealed that duckweed strongly shapes its bacterial microbiome and forms distinct associations with bacterial community members from the initial inoculum. Additionally, these inoculation studies showed the bacterial communities of different duckweed species were similar in taxa composition and abundance. Analysis across the different duckweed bacterial communities collected in this study identified a set of "core" bacterial taxa consistently present on duckweed irrespective of the locale and context. Furthermore, comparison of the duckweed bacterial community to that of rice and Arabidopsis revealed a conserved taxonomic structure between the duckweed microbiome and the terrestrial leaf microbiome. Our results suggest that duckweeds utilize similar bacterial community assembly principles as those found in terrestrial plants and indicate a highly conserved structuring effect of leaf tissue on the plant microbiome.

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“…miRNAs are involved in chlorophyll, carotenoid, and anthocyanin biosynthesis. miRNAs can regulate coloration and chlorophyll accumulation [3,[5][6][7]. Overexpression of osa-miR171b by an arti cial miRNA could enhance chlorophyll accumulation in rice leaves [7].…”

Section: Introductionmentioning

confidence: 99%

Integrated sRNAome and RNA-Seq Analysis Reveals miRNA Effects on Betalain Biosynthesis in Pitaya

Chen

Xie

Hua

et al. 2020

Preprint

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Background: MicroRNAs (miRNAs) and their regulatory functions in anthocyanin, carotenoid, and chlorophyll accumulation have been extensively characterized in many plant species. However, the miRNA regulatory mechanism in betalain biosynthesis remains mostly unknown. Results: In this study, 126 conserved miRNAs and 41 novel miRNAs were first isolated from Hylocereus monacanthus, among which 95 conserved miRNAs belonged to 53 miRNA families. 34 candidate miRNAs related to betalain biosynthesis were differentially expressed. The expression patterns of those differential expressed miRNAs were analyzed in various pitaya tissues by RT-qPCR. A significantly negative correlation was detected between the expression levels of half those miRNAs and corresponding target genes. Target genes of miRNAs i.e. Hmo-miR157b-HmSPL6-like, Hmo-miR160a-Hpcyt P450-like3, Hmo-miR6020-HmCYP71A8-like, Hmo-novel-2-HmCYP83B1-like, Hmo-novel-15-HmTPST-like, Hmo-miR828a-HmTT2-like, Hmo-miR858-HmMYB12-like, Hmo-miR858-HmMYBC1-like and Hmo-miR858-HmMYB2-like were verified by 5′RACE and transient expression system in tobacco.Conclusions: Hmo-miR157b, Hmo-miR160a, Hmo-miR6020 Hmo-novel-2, Hmo-novel-15, Hmo-miR828a and Hmo-miR858 play important roles in pitaya fruit coloration and betalain accumulation. Our findings provide new insights into the roles of miRNAs and their target genes of regulatory functions involved in betalain biosynthesis of pitaya.

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Efficient transformation and artificial miRNA gene silencing in Lemna minor

Cited by 60 publications

References 34 publications

Field Guide to Plant Model Systems

Field Guide to Plant Model Systems

Duckweed hosts a taxonomically similar bacterial assemblage as the terrestrial leaf microbiome

Integrated sRNAome and RNA-Seq Analysis Reveals miRNA Effects on Betalain Biosynthesis in Pitaya

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