Efficient TDZ-induced regeneration from capitulum explants of <i>Gerbera jamesonii</i> Bolus ex Hooker F. - an ornamental plant with high aesthetic value

Talla, Sai Krishna; Madam, Ebenezer; Manga, Sujatha; Aileni, Mahender; Mamidala, Praveen

doi:10.1080/11263504.2018.1539040

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…An analogous result was reported in gerbera wherein the maximum number of roots (~5) with a root length of 6.2 cm in a much higher concentration of IAA (3 mg/l) was recorded when compared to NAA [ 22 ]. The promotional effect of IAA was also reported by another researcher in gerbera, wherein ½MS medium supplemented with 1 mg/l IAA resulted in 95% rooting frequency [ 23 ].…”

Section: Discussionmentioning

confidence: 58%

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Gantait

Mahanta

2021

Journal of Genetic Engineering and Biotechnology

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Background Gerbera jamesonii Bolus ex Hooker f. (African daisy) is listed among the top five most important ornamental plants in the global floricultural industry. To satisfy its demand, the floriculture industry relies on reproducible and effective propagation protocol while retaining the genetic uniformity of G. jamesonii. The present study, for the first time, reports the potential of picloram for enhanced induction of organogenic calli from leaves of G. jamesonii and its high-frequency indirect regeneration. Results The fastest induction of calli with maximum fresh and dry weight was recorded in the Murashige and Skoog (MS) semisolid medium supplemented with 1 mg/l picloram. In addition, callus induction was observed in 2,4-dichlorophenoxy acetic acid- and α-napthaleneaceticacid-supplemented media but with delayed response and reduced fresh and dry weight. The proliferated calli were transferred to shoot induction media containing MS salt and 0.5–1 mg/l N6-benzylaminopurine, kinetin, or thidiazuron. A mean number of ~6 shoots per callus were developed after 5 days of culture in the MS medium supplemented with 1 mg/l kinetin, with a mean length of 5.2 cm. Successful rooting of shoots was achieved in the MS medium fortified with 1.5 mg/l indole-3-acetic acid, wherein the earliest root initiation (~5 days), as well as the maximum number (~9) and length (~4.8 cm) of roots, were recorded. Complete plantlets were primarily acclimatized in sand before being transferred to a mixed substrate (of soil, sand, tea leaf waste, and cow urine) that secured >90% survival and further growth of the plantlets. Eventually, clonal fidelity of the in vitro regenerants assessed via inter-simple sequence repeats (ISSR) primers exhibited a monomorphic banding patterns that suggested genetic integrity within the plantlets as well as with their mother plant. Conclusions The results of the present study should be of interest for commercial propagation and mutagenesis- as well as genetic transformation-related research.

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Section: Discussionmentioning

confidence: 58%

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Gantait

Mahanta

2021

Journal of Genetic Engineering and Biotechnology

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“…TDZ, a cytokinin-like compound, promotes a diverse array of morphogenic responses including shoot bud proliferation [30,31]. Apart from its cytokinin-like activity, TDZ plays important role in modulation of endogenous hormone levels especially auxin/cytokinin ratio [32].…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

Soni

Keswani

Bhatt

et al. 2021

Heliyon

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An efficient and reproducible protocol for in vitro propagation of Dolichandra unguis-cati has been established for the first time from nodal segments. In order to enhance survival rate under ex vitro conditions, photosynthetic potential of in vitro grown plantlets was also studied through JIP test based analysis of polyphasic OJIP chlorophyll a fluorescence OJIP transients, density of active reaction centers, light harvesting efficiency, electron transfer rate, dissipation energy, maximum quantum yield of primary PSII photochemistry and photosynthetic performance index. The best morphogenetic in term of explants response (92.2 %), shoot number (3.43 AE 0.07) and shoot length (4.7 AE 0.31 cm) was obtained on Murashige and Skoog medium supplemented with 0.5 mg l À1 BAP and 1.0 mg l À1 TDZ. The shoots exhibited high frequency rhizogenesis on half strength medium augmented with 2.0 mg l À1 IAA. In vitro plantlets developed highest rate of photosynthesis on day 18 after the initiation of rhizogenesis. High survival rate (96.16%) under ex vitro conditions was observed when in vitro plantlets having high photosynthetic efficiency (Fv/Fm > 0.75) were subjected to hardening and acclimatization process. Plantlets with reduced photosynthetic performance exhibited low survival rate under natural conditions. The developed in vitro protocol will be useful for genetic improvement and multiplication of D. unguis-cati. The results of this study also show that photosynthetic screening of in vitro developed plantlets is highly essential after the rhizogenesis process to achieve higher survival rate under field conditions.

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“…'Mammut' obtained from MS medium supplemented with 0.5 mg L -1 and 0.1 mg L -1 thidaizuron (TDZ) respectively. Bhatia, Singh and Singh (2012) and Talla et al (2019) reported ~16 shoots per capitulum explant of gerbera 'Cabana' (MS+ 5 mg L −1 BAP + 0.2 mg L −1 IAA) and 'Terra Regina' (MS+ 4 mg L −1 BAP + 0.2 mg L −1 α-naphthalene acetic acid). Mosqueda Frómeta et al ( 2017) used modified MS medium with half the level of nitrate along with 800 mg L −1 calcium nitrate, 1.0 mg L −1 BAP, 0.5 mg L −1 NAA for shoot multiplication from a shoots with at least one leaf blade in a temporary immersion bioreactor system, which produce 9 shoots/explant.…”

Section: Culture Initiationmentioning

confidence: 99%

“…The shoots cultured on medium without auxin showed poor rooting frequency about 16% and ~38% in full strength and half-strength MS, respectively. Reducing the salt concentration of MS is a routine practice for in vitro rooting of gerbera (Talla et al, 2019) because the nutrient requirement for root formation is much lower than that of shoot regeneration (Driver;Suttle, 1987). Enrichment of half-strength MS with either indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) had a substantial effect on in vitro rooting (Table 3; Figure 1D).…”

Section: Rooting and Acclimatizationmentioning

confidence: 99%

“…In the present study, we have used capitulum explants to develop a micropropagation protocol for this commercially important cultivar. Although, earlier attempts have been made for micropropagation of gerbera using capitulum (Pierik;Steegmans;Marelis, 1973;Pierik, 1975;Tyagi;Kothari, 2004;Ray;Saha;Roy, 2005;Mohammed;Özzambak, 2007;Chakrabarty;Datta, 2008;Askari Rabori;Fotouhi Ghazvini, 2009;Bhatia et al, 2009Bhatia et al, , 2011Bhatia et al, , 2012Fakhrfeshani et al, 2012;Shylaja et al, 2014;Gautam et al, 2017;Rashmi et al, 2018;Talla et al, 2019;Yuniarto;Soehendi, 2020). It is also essential to optimize the conditions for acclimatization and flowering potential of micropropagated plants in the field condition (Cardoso et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic effect of benzylaminopurine and meta-Topolin combination for micropropagation of gerbera ‘Pink Melody’

Shaheen

Dewir

Kher³

et al. 2022

Ciênc. agrotec.

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Gerbera (Gerbera jamesonii Bolus ex Hook. F.; Asteraceae), is one of the most economically important ornamental plants due to its aesthetic value. In the present study, we established a micropropagation method for the large-scale production of quality planting material of gerbera ‘Pink Melody’. Eighty-six percent of the capitulum explants produced adventitious shoots (15.44 ± 0.34 shoots per capitulum) on Murashige and Skoogs (MS) medium containing 2 mg L−1 6-benzylaminopurine (BAP) after six weeks of incubation. The highest shoot multiplication rate (17 shoots per explant) was obtained on MS medium supplemented with BAP and meta-Topolin (each at 2 mg L−1) after eight weeks. The micro-shoots were successfully rooted (91.35%) on half-strength MS medium containing 2 mg L−1 indole-3-butyric acid (IBA) within four weeks. The micropropagated plantlets were acclimatized with a 97.5% survival rate and produced flowers with no visible morphological aberrations.

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Efficient TDZ-induced regeneration from capitulum explants of Gerbera jamesonii Bolus ex Hooker F. - an ornamental plant with high aesthetic value

Cited by 10 publications

References 28 publications

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera

In vitro propagation and analysis of mixotrophic potential to improve survival rate of Dolichandra unguis-cati under ex vitro conditions

Synergistic effect of benzylaminopurine and meta-Topolin combination for micropropagation of gerbera ‘Pink Melody’

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