Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum

Smaali, Issam; Maugard, Thierry; Limam, Férid; Legoy, Marie‐Dominique; Marzouki, M. Néjib

doi:10.1007/s11274-006-9185-6

Cited by 25 publications

(13 citation statements)

References 9 publications

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“…This result is consistent with the reported enzymes from Stachybotrys microbispora and zygomycete R. miehei [18,21]. Krisch and other groups showed that after 35% (350 mg/ml) cellobiose was mixed with b-glucosidases for 72 h, there was 86 mg/ml cellotriose generated [18,21,22]. Cellobiose may serve as the glucosyl donor in the transglucosylation by b-glucosidase.…”

Section: Transglucosylationsupporting

confidence: 91%

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Lü¹,

Du²,

Wei³

et al. 2013

ABBS

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A b-glucosidase gene unbgl1A was isolated by the functionbased screening of a metagenomic library and the enzyme protein was expressed in Escherichia coli, purified, and biochemically characterized. The enzyme Unbgl1A had a K m value of 2.09 + + + + + 0.31 mM, and a V max value of 183.90 + + + + + 9.61 mmol min 21 mg 21 under the optimal reaction conditions, which were pH 6.0 at 508 8 8 8 8C. Unbgl1A can be activated by a variety of monosaccharides, disaccharides, and NaCl, and exhibits a high level of stability at high concentration of NaCl. Two prominent features for this enzyme are: (i) high glucose tolerance. It can be tolerant to glucose as high as 2000 mM, with K i 5 1500 mM; (ii) high NaCl tolerance. Its activity is not affected by 600 mM NaCl. The enzyme showed transglucosylation activities resulting in the formation of cellotriose from cellobiose. These properties of Unbgl1A should have important practical implication in its potential applications for better industrial production of glucose or bioethanol started from lignocellulosic biomass.

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Section: Transglucosylationsupporting

confidence: 91%

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Lü¹,

Du²,

Wei³

et al. 2013

ABBS

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“…Filamentous fungi S. sclerotiorum strain used in this study was isolated from sunflower Helianthus annuus and kindly provided by Professor Hajlaoui Mohamed Rabeh (Laboratory of Plant Protection, National Institute of the Agronomic Research of Tunisia, Tunis). The strain has been deposited in the VKM collection under the number VKM F‐879.…”

Section: Methodsmentioning

confidence: 99%

An improved method for the production of fructooligosaccharides by immobilized β‐fructofuranosidase from Sclerotinia sclerotiorum

Mouelhi

Abidi

Marzouki

2015

Biotech and App Biochem

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This work is focused on the prebiotic synthesis by a purified immobilized β-fructofuranosidase (FFase) using a by-product molasses as a substrate. When cultivated on wheat bran, the fungus Sclerotinia sclerotiorum produces FFase with interesting transfructosylating activity. The enzyme was purified by gel filtration and anion exchange chromatography to homogeneity. It showed a specific activity of 66.06 U/mg and a molecular mass of 50 kDa. The FFase was immobilized covalently on alginate and chitosan, and the immobilization yield was 90% and 81% respectively, yet the immobilization efficiency was 52% and 93% in that order. The fixed enzymes were stable at a pH varying from 4.0 to 7.0 and at a temperature ranging from 4 to 70 °C. Yet, kinetic parameters and catalytic efficiency were determined for both immobilized and free FFases. Interestingly, chitosan cross-linked enzyme activity was maintained at 89.24% level after 50 reuses during 1 week. Continuous production of fructooligosaccharides (FOS) from beet molasses in chitosan enzyme reactor was improved. The maximum production yield obtained in 12 H was 72.2% (g FOS/g Sucrose). Thin-layer chromatography analysis showed that the major products are kestose and nystose.

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“…A yield of over 90% has been reported for methyl-β-D-fucoside using the Paenibacillus enzyme [59].The yields (Table 2) for higher chain alkyl glucosides range from 1.9% (decyl glucoside) to 36% (for butyl glucoside) in the reverse hydrolysis approach [19,21,54,55]. In the transglycosylation route (Table 3), the reported yield ranges from 4-97% [5,16,17,19,20,24,[26][27][28][29]53,[55][56][57][58][59][60][61][62][63][64][65][66][67][68][69][70]. As observed in Tables 2 and 3, majority of these synthesis reactions have been carried out using purified enzymes.…”

Section: Advantages Of β-Glycosidase Driven Synthesismentioning

confidence: 99%

β-Glycosidases: An alternative enzyme based method for synthesis of alkyl-glycosides

Rather

Mishra

2013

Sustain Chem Process

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Alkyl-glycosides and-polyglycosides are environment friendly, non-ionic surfactants with favourable properties like biodegradability and chemical stability. These are extensively used in personal care products, pharmaceutical preparations and membrane protein research. Commercial production of these surfactants is carried out in multiple steps through Fischer glycosylation reactions under extreme conditions in the presence of toxic catalysts. β-glycosidases provide an alternative enzymatic method for their synthesis as these are easily available from microbial systems and exhibit broad substrate specificity and high stereo-selectivity. This review highlights the recent progress in glycosidase catalyzed synthesis of alkyl-glycosides. Several reaction parameters that affect the overall reaction kinetics, such as, water activity, nature of the glycosyl donor, pH of the reaction medium, reaction time, temperature and source of enzyme are discussed. Strategies available to enhance the yield, including two-phase solvent systems and immobilization are described. Current challenges and future prospects in the biological routes of synthesis are also reviewed.

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Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum

Cited by 25 publications

References 9 publications

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

An improved method for the production of fructooligosaccharides by immobilized β‐fructofuranosidase from Sclerotinia sclerotiorum

β-Glycosidases: An alternative enzyme based method for synthesis of alkyl-glycosides

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Efficient synthesis of gluco-oligosaccharides and alkyl-glucosides by transglycosylation activity of β-glucosidase from Sclerotinia sclerotiorum

Cited by 25 publications

References 9 publications

Expression and characterization of a novel highly glucose-tolerant &beta;-glucosidase from a soil metagenome

Expression and characterization of a novel highly glucose-tolerant &beta;-glucosidase from a soil metagenome

An improved method for the production of fructooligosaccharides by immobilized β‐fructofuranosidase from Sclerotinia sclerotiorum

β-Glycosidases: An alternative enzyme based method for synthesis of alkyl-glycosides

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Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome