Efficient synthesis of enantiopure amines from alcohols using restingE. colicells and ammonia

Abstract

E. coli cells co-expressing alcohol dehydrogenases and an amine dehydrogenase convert alcohols into enantiopure amines at the expense of ammonia.

Highly selective synthesis of d-amino acids from readily available l-amino acids by a one-pot biocatalytic stereoinversion cascade

“…1,4,20 Cascade biocatalysis has been developed in recent years as a promising method for the efficient synthesis of pharmaceutical intermediates and ne chemicals. [21][22][23][24] Cascade biocatalysis comprises multiple biocatalytic reactions in a single reaction vessel without the isolation of any intermediate, and starting from cheap and available substrates. 21,23,25 Because L-amino acids are mostly available through fermentation from inexpensive and renewable natural sources, multi-enzyme cascades for the biocatalytic stereoinversions of L-amino acids into Damino acids represent economically effective and environmentally benign methods.…”

Sequential Two‐Step Stereoselective Amination of Allylic Alcohols through the Combination of Laccases and Amine Transaminases

“…Biocatalytic methods have emerged as a sustainable solution for the synthesis of chiral amines by means of the single action of enzymes of several classes, including lipases, amine transaminases (ATAs), amine oxidases, amine dehydrogenases, imine reductases or reductive aminases, but, interestingly, great efforts have in recent years been devoted to the synthesis of chiral amines through chemo‐, photo‐ and multienzymatic approaches . As an example, the selective amination of racemic sec ‐alcohols through elegant cascades based on the combination of alcohol‐dehydrogenase‐catalysed oxidation to ketones with subsequent bioamination by using ATAs or amine dehydrogenases has been described. In spite of these efforts, the biocatalytic synthesis of (3 E )‐4‐arylbut‐3‐en‐2‐amines has received little attention, the activity of commercially available amine transaminases for the bio‐transamination of (3 E )‐4‐phenylbut‐3‐en‐2‐one into optically active (3 E )‐4‐phenylbut‐3‐en‐2‐amine being low (<30 %) …”

Control of Absolute Stereochemistry in Transition‐Metal‐Catalysed Hydrogen‐Borrowing Reactions

“…[37] Recently,i th as even been shown that amination is possible in E. coli cells. [38] As this chemistry does not involvet ransition-metal catalysis, ac omprehensive discussion lies outside the scope of this Minireview,b ut enzymatic chemistry can offer au seful alternative to transitionmetal-catalysed methods.…”