Efficient surface modification of biomaterial to prevent biofilm formation and the attachment of microorganisms

Bazaka, Kateryna; Jacob, Mohan V.; Crawford, Russell J.; Ivanova, Elena P.

doi:10.1007/s00253-012-4144-7

Cited by 204 publications

(143 citation statements)

References 160 publications

(157 reference statements)

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“…2,21,22 However, information on the effect of nanoHA surface properties on bacterial adhesion and biofilm formation is scarce. 20 In this work, the purpose was to study the influence of nanoHA surface characteristics in S. epidermidis RP62A biofilm formation. Therefore, two different initial inoculum concentrations (Ci) were used in order to check if these Ci would affect the biofilm formed on nanoHA surfaces differing exclusively on the effect of different sintering temperatures, while both keeping grain size within the nanoscale.…”

Section: Discussionmentioning

confidence: 99%

“…While evidently detrimental to the healing process and overall wellbeing of the patient, bacterial attachment, while ensuring the formation of a biofilm, may also damage the performance of the device. 20 NanoHA has been used as coating or bone substitute in orthopaedic devices, due to its properties such as biocompatibility, bioactivity, osteoconductivity and osteoinduction. 2,21,22 However, information on the effect of nanoHA surface properties on bacterial adhesion and biofilm formation is scarce.…”

Section: Discussionmentioning

confidence: 99%

“…The material Ra has been pointed out as cap-able of influencing bacterial adhesion, proliferation, biofilm formation and detachment of adherent bacteria to a given material. 20,43,44 This is probably due to the fact that rough surfaces have greater surface areas 35 and that depressions in the roughened surfaces provide more favorable sites for colonization, as crevices may protect bacteria from shear stresses. 18,23 In our study, a correlation between roughness, surface area and amount of bacteria was observed, with nanoHA1000 displaying higher average roughness values and, simultaneously, larger amount of bacterial accumulation.…”

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confidence: 99%

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Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation

et al. 2013

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Nanohydroxyapatite (nanoHA), due to its chemical properties, has appeared as an exceptionally promising bioceramic to be used as bone regeneration material. Staphylococcus epidermidis have emerged as major nosocomial pathogens associated with infections of implanted medical devices. In this work, the purpose was to study the influence of the nanoHA surface characteristics on S. epidermidis RP62A biofilm formation. Therefore, two different initial inoculum concentrations (Ci) were used in order to check if these would affect the biofilm formed on the nanoHA surfaces. Biofilm formation was followed by the enumeration of cultivable cells and by scanning electron microscopy. Surface topography, contact angle, total surface area and porosimetry of the biomaterials were studied and correlated with the biofilm data. The surface of nanoHA sintered at 830 o C (nanoHA830) showed to be more resistant to S. epidermidis attachment and accumulation than that of nanoHA sintered at 1000 o C (nanoHA1000). The biofilm formed on nanoHA830 presented differences in terms of structure, surface coverage and EPS production when compared to the one formed on nanoHA1000 surface. It was observed that topography and surface area of nanoHA surfaces had influence on the bacterial attachment and accumulation. Ci influenced bacteria attachment and accumulation on nanoHA surfaces over time. The choice of the initial inoculum concentration was relevant proving to have an effect on the extent of adherence thus being a critical point for human health if these materials are used in implantable devices. This study showed that the initial inoculum concentration and surface material properties determine the rate of microbial attachment to substrata and consequently are related to biofilm-associated infections in biomaterials.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation

et al. 2013

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“…4 In biomedical field, plasma coatings can be used to afford material chemical or mechanical protection, impart biocompatibility and antimicrobial activity, and for drug delivery. 5,6 In electronics, films from organic precursors have been used to replace conventional inorganic materials to produce superior organic and hybrid devices. Organic electronics offer several advantages over conventional silicon electronics, such as flexibility and low cost fabrication from solution, allowing production of flexible, lightweight, self-illuminating displays based on Organic Light Emitting Diode (OLED) and Organic Field Effect Transistor (OFET) devices and low-cost Organic Photo-Voltaic (OPV) and sensors.…”

Section: Introductionmentioning

confidence: 99%

RF plasma polymerised thin films from natural resources

Jacob

Easton

Anderson

et al. 2014

Int. J. Mod. Phys. Conf. Ser.

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Plasma polymerisation is an effective tool for fabrication of thin films from volatile organic monomers. RF plasma assisted deposition is used for one-step, chemical-free polymerisation of nonsynthetic materials derived directly from agricultural produces. By varying the deposition parameters, especially the input RF power, the film properties can be tailored for a range of uses, including electronics or biomedical applications. The fabricated thin films are optically transparent with refractive index close to that of glass. Given the diversity of essential oils, this paper compares the chemical and physical properties of thin films fabricated from several commercially exploited essential oils and their components. It is interesting to note that some of the properties can be tailored for various applications even though the chemical structure of the derived polymer is very similar. The obtained material properties also show that the synthesised materials are suitable as encapsulating layers for biodegradable implantable metals.

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“…Then, some reactive oxygen species (ROS) are produced. One target is the outer membrane of bacteria (Bazaka et al, 2012). Observation of cells emptied of their cytoplasmic content suggested that the outer membrane was damaged.…”

Section: Discussionmentioning

confidence: 99%

Bactericidal efficiency of UV-active TiO2 thin films on adhesion and viability of food-borne bacteria

Barthomeuf¹,

Raymond²,

Castel³

et al. 2015

International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork

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Biofilms, containing pathogenic bacteria, represent a recurrent economic and safety problem in food industries, due to their high resistance to cleaning and sanitizing procedures. The development of photoactive surfaces with bactericidal property could facilitate the elimination of such microbial biofilms. One solution may be to deposit a photocatalyst top-layer (TiO 2 ) on conventional materials used in food plants. Our aim is to study the photocatalytic activity of such layers on the adhesion and viability of different bacteria present on food plants, especially in pork meat factory: Listeria monocytogenes, Yersinia enterocolitica and Pseudomonas fragi. Glass substrates were coated with TiO 2 thin films by radio-frequency magnetron sputtering under various deposition conditions (deposition temperature T, oxygen partial pressure P O2 ). The characterization of the TiO 2 thin layers was performed using spectrophotometry, scanning electron microscopy and X-ray diffraction analysis. And photocatalytic activity under UVA illumination (365 nm) has been checked for all samples. Bactericidal activity has been demonstrated on the bacteria tested by enumeration of the adherent cells and in situ fluorescent labeling after three hours of contact with the thin film and a subsequent UVA illumination. Adherent bacteria with damaged bacterial cell wall were observed using a scanning electron microscopy; this can be associated with presence of oxidative stress due to the photocatalytic activity of the TiO 2 thin layer. The selected TiO 2 coating presents a photocatalytic activity leading to an oxidative stress. This activity provides bactericidal properties against different strains from the meat industry. This thin layer could be optimized by modifying anionic composition (band-gap reduction) during coating in order to be active under solar light so it could be used to fight against biofilms.

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Efficient surface modification of biomaterial to prevent biofilm formation and the attachment of microorganisms

Cited by 204 publications

References 160 publications

Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation

Influence of nanohydroxyapatite surface properties on Staphylococcus epidermidis biofilm formation

RF plasma polymerised thin films from natural resources

Bactericidal efficiency of UV-active TiO2 thin films on adhesion and viability of food-borne bacteria

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