Efficient subtyping of Yersinia enterocolitica bioserotype 4/O:3 with pulsed-field gel electrophoresis

Abstract

The purpose of this study was to evaluate the efficiency of pulsed‐field gel electrophoresis (PFGE) for differentiation of isolates of Yersinia enterocolitica bioserotype 4/O:3 recovered from pig tongues at retail level in the Helsinki area during October and November of 1996. NotI generated 15 different PFGE patterns for 128 isolates. The discriminatory index did not exceed 74% due to the presence of two predominant PFGE‐types, NA1 (58/128) and NB1 (25/128). After preliminary investigations with 35 enzymes, ApaI, XbaI, XhoI and SpeI were chosen for further characterization of the isolates. The discriminatory index increased from 74% to 93% and the number of different genotypes from 15 to 30 when isolates with the same PFGE pattern with NotI were further characterized with ApaI and XhoI, indicating that PFGE can be an efficient technique for characterization of bioserotype 4/O:3.

Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Yersinia enterocolitica subsp. palearctica and Its Application to Bioserogroup 4/O3 Subtyping

“…A local reference strain from which amplicons have been sequenced is sufficient for gel-to-gel comparison. Since the results of MLVA are integers, the genotypes determined in different laboratories may be easily compared and joint databases can be produced, as shown in various studies (18,29 (11). Overall, the data showed that on a countrywide scale, MLVA would offer a higher discriminatory power for Y. enterocolitica 4/O3 than the methods mentioned above.…”

“…Biochemical differentiation (biotyping), the most simple and widely available method, is able to separate Y. enterocolitica O3 into biotypes 2, 3, and 4, with biotype 4 (BT4) causing the majority of clinical cases (11,12,20). Most Y. enterocolitica 4/O3 isolates belong to phage type VIII (21).…”

“…Eleven NotI pulsotypes were distinguished among 20 strains of bioserogroup 4/O3 of worldwide origin (21). Application of an additional enzyme was reported to improve the discriminatory power of PFGE (1,11). The recently developed fluorescent amplified fragment length polymorphism method demonstrated a relatively high discriminatory power by distinguishing 13 genotypes among 17 strains of Y. enterocolitica 4/O3 (10).…”

Multiple-Locus Variable-Number Tandem-Repeat Analysis in Genotyping Yersinia enterocolitica Strains from Human and Porcine Origins

“…The strains belonged to serotypes O:3 (n ϭ 363), O:9 (n ϭ 9), and O:5,27 (n ϭ 7). We previously divided all serotype O:3 strains into 81 genotypes using PFGE with the enzyme NotI, and we divided 206 of those strains into 105 genotypes using the additional enzymes ApaI and XhoI (16,17,(24)(25)(26). The strains were grown on tryptic soy agar (Difco, Lawrence, KS) at 28°C for 18 to 24 h, and the DNA was extracted using guanidium thiocyanate (27).…”

Clonal Dissemination of Yersinia enterocolitica Strains with Various Susceptibilities to Nalidixic Acid

“…The epidemiological studies clearly showed a close epidemiological relationship among the analyzed strains. The PFGE method has been extensively used to analyze the epidemiological relationship among strains of Y. enterocolitica (1,6,8,11). In our study the same discriminatory power for both REP-PCR and PFGE was observed.…”