Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

Foo, Su Chern; Yusoff, Fatimah Md.; Ismail, Maznah; Basri, Mahiran; Khong, Nicholas M.H.; Chan, Kim Wei; Yau, Sook Kun

doi:10.1016/j.apjtb.2015.06.003

Cited by 48 publications

(28 citation statements)

References 29 publications

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“…Consequently, 25.18% of CME was obtained from the freeze dried biomass of C. calcitrans. Also, results from our previous study support that methanolic extracts had significantly higher (p b 0.05) extractability (22.71%) and bioactives than some investigated solvent mixtures (9:1 v/v acetone and water or 9:1 v/v acetone and chloroform) [9]. The extractable yield was comparably higher than the methanolic extracts of the brown macroalgae, Sargassum polycystum (4.05%) [29] as well as commercial microalgae, Spirulina platensis (7.3%) [30].…”

Section: Extraction and Fractionation Yieldssupporting

confidence: 53%

“…Quantification of fucoxanthin in C. calcitrans extract/fraction using high performance liquid chromatography with a diode array detector (HPLC-DAD) was conducted following the method by Foo et al [9]. An Agilent 1200 series (G1329-90010) autosampler was used to inject samples into an Agilent 1200 series HPLC (Agilent Technologies Inc., Alpharetta, GA, USA) equipped with a DAD G1351B/1365B diode array detector.…”

Section: Fucoxanthin Quantificationmentioning

confidence: 99%

“…The crude methanolic extract (CME) was obtained following the method by Foo et al [9]. Following this, the fractionation procedure was done by fully dispersing 1 g of CME in 25 ml distilled water in a 250 ml Duran® laboratory bottle.…”

Section: Production Of Fucoxanthin-rich Fraction (Fxrf) From C Calcimentioning

confidence: 99%

“…The marine diatom, C. calcitrans (UPMC-A0010) was mass cultured in 120 l capacity annular photobioreactors following the method by Foo et al [9] [18]. Conway medium is a common marine algae culture medium widely used for the production of live feeds [19] in commercial culture of marine invertebrate larvae [20].…”

Section: Microalgae Biomass Cultivation and Collectionmentioning

confidence: 99%

“…An added advantage of culturing diatoms in closed photobioreactors is the availability of a stable and sustainable supply of biomass all year around. Findings from our previous study [9] revealed that crude methanolic extracts (CME) of C. calcitrans not only contained the highest amount of carotenoids but also championed other solvent extracts in terms of antioxidant activities.…”

Section: Introductionmentioning

confidence: 97%

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Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

et al. 2015

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Fucoxanthin is the major carotenoid produced by diatoms and shown to exhibit potent anti-obesity and anticancer activities. This study presents a simple protocol for the preparation of fucoxanthin-rich fraction (FxRF) with high antioxidative properties from a selected marine diatom, Chaetoceros calcitrans. Fucoxanthin concentrates were prepared from crude methanolic extract (CME) with liquid-liquid partitioning of three types of solvent systems (i.e. diethyl ether-water, dichloromethane-water and ethyl acetate-water), followed by comparative antioxidant evaluation. Dichloromethane fraction (DCMF) was found to contain significantly higher (p b 0.05) level of carotenoid (7.13 ± 0.01 mg·g −1 DW) and fucoxanthin (5.25 ± 0.03 mg FX·g −1 DW) contents accompanied with elevated (p b 0.05) antioxidant activities (DPPH• and ABTS• + radical scavenging, and beta carotene bleaching assays) as compared to other tested fractions. This study showed that FxRF with enhanced antioxidant properties could be effectively produced and concentrated through the DCM-H 2 O partition of CME.

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Section: Extraction and Fractionation Yieldssupporting

confidence: 53%

Section: Fucoxanthin Quantificationmentioning

confidence: 99%

Section: Production Of Fucoxanthin-rich Fraction (Fxrf) From C Calcimentioning

confidence: 99%

Section: Microalgae Biomass Cultivation and Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

et al. 2015

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Extract of the Microalga Nitzschia laevis Prevents High‐Fat‐Diet‐Induced Obesity in Mice by Modulating the Composition of Gut Microbiota

Guo

Liu

Wei

et al. 2018

Molecular Nutrition Food Res

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Scope The aim of the present study is to investigate the efficacy of Nitzschia laevis extract (NLE) in preventing obesity in mice fed with a high‐fat diet (HFD), and the potential underlying mechanisms focusing on modulation of the gut microbiota profile. Methods and results Physiological, histological, and biochemical parameters and gut microbiota compositions are compared among four experimental groups fed respectively with the following diets for 8 weeks: Normal chow diet, HFD, HFD + low concentration of NLE, and HFD + high concentration of NLE. The results demonstrate that NLE supplementation significantly reduces body weight gain and effectively prevents lipid accumulation in the white adipose tissue and liver of the mice. Mechanistic analysis reveals that NLE promotes the expression of uncoupling protein 1 and peroxisome proliferators‐activated receptor‐γ coactivator‐1 mRNA in brown adipose tissue. Furthermore, NLE protects the gut epithelium and positively reshapes the gut microbiota composition against the damaging effect of HFD. Conclusions NLE supplementation demonstrates a protective effect against HFD‐induced obesity in mice, which is associated with reshaping the profile of gut microbiota. To the best of our knowledge, this has been the first report on the potential of microalgal extract to prevent obesity by modulating gut microbiota.

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Optimising biomass and peridinin accumulation in the dinoflagellate Symbiodinium voratum using a twin-layer porous substrate bioreactor

Langenbach

Melkonian

2018

J Appl Phycol

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Efficient solvent extraction of antioxidant-rich extract from a tropical diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

Cited by 48 publications

References 29 publications

Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

Production of fucoxanthin-rich fraction (FxRF) from a diatom, Chaetoceros calcitrans (Paulsen) Takano 1968

Extract of the Microalga Nitzschia laevis Prevents High‐Fat‐Diet‐Induced Obesity in Mice by Modulating the Composition of Gut Microbiota

Optimising biomass and peridinin accumulation in the dinoflagellate Symbiodinium voratum using a twin-layer porous substrate bioreactor

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