Efficient Shoot Regeneration System of Pear Rootstock Ohf 333 (Pyrus Communis L.) Leaves

Nacheva, L.; Gercheva, P.; Dzhuvinov, V.

doi:10.17660/actahortic.2009.839.23

Cited by 11 publications

(5 citation statements)

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“…The plant growth regulator types and concentrations are the most likely cause, since the CL basal medium has consistently resulted in a high level of regeneration of several cultivars (Chevreau et al 1997;Malnoy et al 2003;Zhu and Welander 2000). Reported regeneration frequencies are comparable to those reported for protocols using MS (Abdollahi et al 2006;Nacheva et al 2009;Shibli et al 2000), Nitsch and Nitsch (Abu-Qaoud et al 1991;Hennayake et al 2003;Matsuda et al 2005;Nitsch and Nitsch 1969;Sun and Sun 1999;Sun et al 2011;Tang et al 2008), Quoirin and Lepovire (Caboni et al 1999;Martinelli et al 2009;Quoirin and Lepoivre 1977), B5 or close derivatives (Gamborg et al 1968;Lane et al 1998;Yancheva et al 2006), or Linsmaier and Skoog (Linsmaier and Skoog 1965;Predieri et al 1989).…”

Section: Discussionmentioning

confidence: 92%

“…IBA has also been used successfully for regeneration of 'Niitaka' (Lee et al 2002). For the OH 9 F 333 pear rootstock, Nacheva et al (2009) used 2.5 lM IBA in combination with 20 lM indole acetic acid (IAA). However, in a direct comparison of 0.54 lM NAA versus IBA, albeit with Murashige and Skoog (MS) medium (Murashige and Skoog 1962) and 26.6 lM BA as the cytokinin, Tang et al (2008) observed a reduction in regeneration frequency from 63 to 24%.…”

Section: Discussionmentioning

confidence: 99%

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Adventitious shoot regeneration of pear (Pyrus spp.) genotypes

Bell

Scorza

Lomberk

2011

Plant Cell Tiss Organ Cult

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Adventitious shoot regeneration of twenty-four pear genotypes was compared in a common in vitro shoot induction and development protocol. This study also compared cultures newly established from scionwood with cultures that been in long-term cold storage. In vitro cultures of 13 Pyrus genotypes and budwood from 23 Pyrus genotypes were obtained from the National Clonal Germplasm Repository (NCGR) in Corvallis, Oregon. With the exception of one genotype of P. elaeagrifolia Pall., and 'Ya Li' (P. pyrifolia var. sinensis Teng & Tanabe), all were P. communis L. cultivars. The basal shoot induction media consisted of Chevreau and Leblay (CL) basal nutrients, vitamins, and organics (Chevreau and Leblay in Acta Hortic 336: [263][264][265][266][267][268] 1993). The analysis of variance indicated that differences among genotypes were highly significant and the main effect of culture origin was nonsignificant. However, there was a significant interaction between genotype and culture origin, with percentage regeneration of 'Abate Fetel' from new budwood significantly greater than that from long-term in vitro cultures, while 'Jesinji Vodenac' cultures derived from the old NCGR cultures regenerated significantly more adventitious shoots. The ranges of mean regeneration frequency were similar for both in vitro (0-87.7%) and scionwood-derived cultures (0-70.7%). Maximum regeneration was observed for 'Conference', followed by 'Magness', 'Dr. Jules Guyot', and Packham's Triumph'. The range of number of adventitious shoots was relatively narrow, with the minimum of 1.0 for seven genotypes to 2.2 for 'Conference'.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Adventitious shoot regeneration of pear (Pyrus spp.) genotypes

Bell

Scorza

Lomberk

2011

Plant Cell Tiss Organ Cult

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“…e in vitro culture was maintained as a 3-week subculture on a modified MS medium (Murashige & Skoog, 1962) as described previously (Dimitrova et al, 2016;Nacheva et al, 2009) with some modifications. Briefly, the concentration of NH 4 NO 3 was reduced to half strength, CaCl 2 was replaced with 1,000 mg L −1 Ca(NO 3 ) 2 , and the medium was supplemented with 2.5 μM 6-benzylaminopurine (BAP), 0.005 μM IBA, 30 g L −1 sucrose, 6.5 g L −1 Phyto agar (Duchefa Biochemie, the Netherlands), at a pH of 5.6.…”

Section: Growing Conditionsmentioning

confidence: 99%

“…However, the widespread use of micropropagated transplants is still limited by the high production costs in relation to low growth rates, a significant loss of plants in vitro due to microbial contamination, poor rooting, low survival percentage at the ex vitro acclimatization stage, and high labor costs. Pear (Pyrus) cultivars and species are oen recalcitrant to tissue culture manipulations, although propagation protocols for different pear genotypes have been reported (Bell & Reed, 2002;Chevreau et al, 1992;Dimitrova et al, 2016;Nacheva et al, 2009;Reed et al, 2013). Similar to other woody species of the Rosaceae family, rooting in vitro has proven to be difficult and highly genotype-dependent (Nemeth, 1986;Reed, 1995).…”

Section: Introductionmentioning

confidence: 99%

An Optimized Micropropagation Protocol by Ex Vitro Rooting of Pear Rootstock OHF 333 (Pyrus communis L.)

Dimitrova

Nacheva

2021

Acta Agrobot

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Pear rootstock OHF 333 ( Pyrus communis L.), which is included in the US ‘Old Home’ × ‘Farmingdale’ series, is characterized by good compatibility with most other varieties, high yields, and a moderate degree of resistance to fire blight. Micropropagation in vitro has shown promise for rapid, large-scale cloning of disease-free plant material throughout the year. However, pear shoots are often recalcitrant to rooting, and this process is highly genotype-dependent. This study aimed to improve the micropropagation protocol by ex vitro rooting of pear rootstock OHF 333 ( Pyrus communis L. ‘Old Home’ × ‘Farmingdale’). Charkor, a new plant growth regulator of natural origin was used, which contains metabolism products of symbiotic fungus-endophytes of ginseng roots, as an alternative to synthetically produced plant growth regulators (PGRs). Microcuttings were obtained from in vitro cultured shoots and subjected to four different treatments for ex vitro rooting: 1 g L −1 1-naphthaleneacetic acid (NAA) (as a powder), 0.5 mL L −1 Charkor for 3 hr or 6 hr, or the same concentration of Charkor prepared as a powder. Microshoots dipped in sterile distilled water with no additional hormonal treatments served as controls. Cultures were kept in a growth chamber under a 16-hr photoperiod, with air humidity maintained close to 100% (above 96%) for 2 weeks and then gradually reduced to 60%. Data on final acclimatization rate (survival rate; %), mean number of roots per plant, stem and root length, mean number of leaves per plant, and final acclimatization rate were collected 90 days after transplanting to ex vitro conditions. All treatments induced a successful acclimatization rate of more than 31%. The highest survival rate (86%) and longest stems were achieved by treatment with 0.5 mL L −1 Charkor for 6 hr. The greatest mean number of roots per plant, root length, and number of leaves was achieved in the variant treated with 1 g L −1 powdered NAA.

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“…However, woody plants are often recalcitrant to in vitro cultivation and this process is highly genotype dependent. Pear rootstocks and varieties are often difficult to cultivate in vitro, although some micropropagation protocols have been published (Chevreau et al, 1992;Bell, Reed, 2002;Nacheva et al, 2009;Reed et al, 2013). During in vitro cultivation, plantlets grow under specific conditions: in small tightly-closed vessels; with high air humidity, low gas exchange and, thus, a CO 2shortage during almost the whole photoperiod; ethylene production and relatively low light intensity; in a culture medium with a large concentration of sugar (Ziv, 1991).…”

Section: Introductionmentioning

confidence: 99%

Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

Dimitrova¹,

Nacheva²,

Berova³

et al. 2021

SBAL

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In vitro micropropagation of plants is highly useful for obtaining large quantities of planting material with valuable economic qualities. However, plantlets grow in vitro in a specific environment and the adaptation after the transfer to ex vitro conditions is difficult. Therefore, the acclimatization is a key step, which mostly determines the success of micropropagation. The aim of this investigation was to study the effect of the biofertlizer Lumbrical on ex vitro acclimatization of micropropagated pear rootstock OHF 333 (Pyrus communis L.). Micropropagated and rooted plantlets were potted in peat and perlite (2:1) mixture with or without Lumbrical. They were grown in a growth chamber at a temperature of 22±2 °C and photoperiod of 16/8 hours supplied by cool-white fluorescent lamps (150 µmol m-2 s-1 Photosynthetic Photon Flux Density, PPFD). The plants were covered with transparent foil to maintain the high humidity, and ten days later, the humidity was gradually decreased. Biometric parameters, anatomic-morphological analyses, net photosynthetic rate and chlorophyll a fluorescence (JIP test) were measured 21 days after transplanting the plants to ex vitro conditions. The obtained results showed that the plants, acclimatized ex vitro in the substrate with Lumbrical, presented better growth (stem length, number of leaves, leaf area and fresh mass) and photosynthetic characteristics as compared to the control plants. This biostimulator could also be used to improve acclimatization in other woody species

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Efficient Shoot Regeneration System of Pear Rootstock Ohf 333 (Pyrus Communis L.) Leaves

Cited by 11 publications

References 0 publications

Adventitious shoot regeneration of pear (Pyrus spp.) genotypes

Adventitious shoot regeneration of pear (Pyrus spp.) genotypes

An Optimized Micropropagation Protocol by Ex Vitro Rooting of Pear Rootstock OHF 333 (Pyrus communis L.)

Biofertlizer Lumbrical improves the growth and ex vitro acclimatization of micropropagated pear plants

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