Efficient Separation and Analysis of Triacylglycerols: Quantitation of β-Palmitate (OPO) in Oils and Infant Formulas

Vyssotski, Mikhail; Bloor, Stephen J.; Lagutin, Kirill; Wong, Herbert; Williams, D. Bradley G.

doi:10.1021/acs.jafc.5b01835

Cited by 16 publications

(14 citation statements)

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“…For example, TAG 32:0 is composed of seven isomers, which cannot be fully separated even with two C18 columns connected in series combined with a shallow gradient elution of 90 min (Figure 1). MS/MS scanning data allowed us to determine the fatty acid composition of the chromatographically resolved and non-resolved isomers [16], but reliable quantification of each isomer is not currently feasible for most TAG groups using one-dimensional LC [15,17]. Consequently, our survey on seasonal variation of TAG profile was conducted at the group level using a short LC method, which allowed all main isomers to be eluted as a single peak.…”

Section: Resultsmentioning

confidence: 99%

“…TAG 52:2 contains two of the most abundant fatty acids of milk C16:0 and C18:1, the position of which in the glycerol backbone has attracted a considerable interest in recent years, due to the differential absorption efficiency by infants of C16:0 fatty acid at sn -2 and sn -1/3 position [14,15]. With the help of the standards, we could reveal the strict control of the ratio of positional isomer produced in the mammary gland for this particular TAG group.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, TAG structure could affect the absorption efficiency of fatty acids by infants [14,15]. Therefore, understanding the accumulation pattern of milk TAG is important for maximizing the functional and nutritional values of milk fat.…”

Section: Introductionmentioning

confidence: 99%

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Seasonal Variation of Triacylglycerol Profile of Bovine Milk

et al. 2017

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Milk contains 3–6% of fat, of which the dominant component is triacylglycerol (TAG). Over 100 TAG groups can be readily detected in any non-enriched milk sample by LC-MS; most TAG groups contain several isomers (TAG molecules with different fatty acid composition), which cannot be fully resolved chromatographically by any single stationary phase. TAG profile of mature milk from 19 cows was surveyed in this study for eight consecutive months using RP-LC-Orbitrap MS. It was found that TAG profile of milk was not constant throughout the milking season and the seasonal pattern varied with TAG groups. The overall unsaturation level of TAG was stable from October 2013 to January 2014, decreased in February/March 2014 and then increased from April and peaked in May 2014. In addition to the seasonal fluctuation in TAG profile, the proportion of different isomeric species within a TAG group also changed substantially across seasons. However, the proportion of different positional isomers within a given TAG group does not seem to vary during the milking season. To our knowledge, this is the first report on the seasonal change of milk lipid at the TAG group and isomer level.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Seasonal Variation of Triacylglycerol Profile of Bovine Milk

et al. 2017

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“…Physico-chemical characteristics of milk fat is determined by the triglycerides [ 10 ]. Functional, processing and quality characteristics of milk and dairy products are also influenced by the triglyceride profile [ 11 ]. During the storage, several biochemical changes take place in the fat fractions of milk, therefore, it is extremely important to study the effect of UHT treatment and storage on triglyceride profile, which is not previously investigated.…”

Section: Introductionmentioning

confidence: 99%

Lipid compositional changes and oxidation status of ultra-high temperature treated Milk

et al. 2018

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BackgroundMilk fat is one of the complex fat and most sensitive biochemical compounds towards auto-oxidation. To enhance the shelf life, milk is subjected to Ultra-high Temperature (UHT) treatment followed by aseptic packaging. During the storage, several chemical and biochemical changes take place in lipid fraction of UHT milk. In current investigation, the effect of UHT treatment and storage was determined by making a comparison in fatty acid profile, triglyceride composition, organic acids and lipid oxidation of the thermally treated and stored milk with raw milk, which was not reported in earlier investigations.MethodsRaw milk samples were collected from the bulk storage facility of a dairy industry. The same milk was routed to UHT treatment and aseptically packaged samples were collected. The fatty acid profile, triglyceride composition, organic acids and lipid oxidation was determined in raw and UHT treated milk at 0, 30, 60 and 90 days. Fatty acid and triglyceride profile was determined on GC-MS while organic acids were determined by HPLC. For the measurement of induction period, professional Rancimat was used. Lipid oxidation was characterized through free fatty acids, peroxide value, anisidine value and conjugated dienes.ResultsCompositional attributes of milk remain unchanged during the entire length of storage. Concentrations of short-chain fatty acids in raw and UHT milk were 10.49% and 9.62%. UHT treatment resulted in 8.3% loss of short-chain fatty acids. Up to 30 days, storage did not have any significant effect on fatty acid profile of UHT milk. Concentration of medium-chain fatty acids in raw and UHT treated milk was 54.98% and 51.87%. After 30, 60 and 90 days of storage, concentration of medium chain fatty acids was found 51.23%, 47.23% and 42.82%, respectively. Concentration of C18:1 and C18:2 in raw and UHT milk was 26.86% and 25.43%, respectively. The loss of C18:1 and C18:2 in UHT treatment was 5.32%. After 30, 60 and 90 days of storage, the concentrations of C18:1 and C18:2 were 24.6%, 21.06% and 18.66%, respectively. Storage period of 30 days was found non-significant, while noticeable variations were found in triglyceride profile of 60 and 90 days old samples of UHT milk. UHT treatment and storage period significantly affected the concentration of organic acids in milk. After UHT treatment, concentration of lactic acid, acetic acid, citric acid, pyruvic acid, formic acid, succinic acid and oxalic acid increased by 3.45, 0.66, 3.57, 0.68, 2.24, 2.16 and 1.63 mg/100 g. Effect of storage period on the production of organic acids in UHT milk was non-significant up to 30 days. After 60 days of storage period, the increase in concentration of lactic acid, acetic acid, citric acid, pyruvic acid, formic acid, succinic acid and oxalic acid was 3.79, 0.75, 4.69, 0.78, 2.83, 3.03 and 2.38 mg/100 g. After 90 days of storage period, the increase in concentration of lactic acid, acetic acid, citric acid, pyruvic acid, formic acid, succinic acid and oxalic acid was 7.3, 2.18, 9.96, 3.58, 11.37, 5.22 and ...

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“…Almeling and Holzgrabe [9] have studied the influence of the experimental parameters on the use of HPLC-ELSD for the quality control monitoring of drug substances, especially the effect of different flow rates of the scavenger (nebulizing) gas. Triacylglycerols in oils and infant formulas have been analyzed by HPLC with an ELSD [10]. Recently, Arslan et al have described the optimization of parameters of an ELSD in a fully automated three-dimensional column-switching SPE-FIA-HPLC system used for lipid characterization [11].…”

Section: Introductionmentioning

confidence: 99%

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

Pazourek

Šmejkal

2016

Molecules

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Abstract:The use of a liquid chromatography (LC) splitter inserted between an HPLC column and an evaporative light scattering detector (ELSD) is described. This paper aims to show the feasibility of using the splitter in an HPLC-ELSD system to fractionate a model mixture of analytes, namely salicin (2-(hydroxymethyl)-phenyl-β-D-glucopyranoside) and glucose. The retention factors and efficiency of the separation were studied under various temperatures and water contents in the mobile phase in order to clarify the mechanism of polyols separation on a diol column under the conditions of hydrophilic liquid chromatography (HILIC). Finally, the system was applied to a biological sample (a lyophilized colony gel of Pectinatella magnifica), where the presence of fructose and glucose was confirmed.

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Efficient Separation and Analysis of Triacylglycerols: Quantitation of β-Palmitate (OPO) in Oils and Infant Formulas

Cited by 16 publications

References 40 publications

Seasonal Variation of Triacylglycerol Profile of Bovine Milk

Seasonal Variation of Triacylglycerol Profile of Bovine Milk

Lipid compositional changes and oxidation status of ultra-high temperature treated Milk

Feasibility of Fraction Collection in HPLC Systems with Evaporative Light Scattering Detector: Analysis of Pectinatella magnifica

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