Efficient saccharification of agave biomass using Aspergillus niger produced low-cost enzyme cocktail with hyperactive pectinase activity

Wang, Jiahong; Chio, Chonlong; Chen, Xuntong; Su, Erzheng; Cao, Fuliang; Jin, Yongcan; Qin, Wensheng

doi:10.1016/j.biortech.2018.09.069

Cited by 43 publications

(28 citation statements)

References 39 publications

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“…(a). It suggested that pectinase secretion was greatly affected by the pH: pH 3 to 5 were more suitable for producing higher activity from the pectinase enzymes than other pH condition, which was consistent with previous research . As Fig.…”

Section: Resultssupporting

confidence: 91%

“…The squared multiple R is 0.961, which indicates that the model can explain the test results well . The P values of ‘regression’ and ‘lack of fit’ were 0.001 (< 0.05) and 0.260 (> 0.05), respectively, which indicated that the model is credible for assessing PG activity . All of 2‐D contour plots are presented as non‐circle graphs (Fig.…”

Section: Resultsmentioning

confidence: 89%

“…Aspergillus niger is one of the most important species in the food industry. It is widely used for the preparation of various enzymes, such as cellulase, hemicellulase, and pectinase . Aspergillus niger is considered to be a safe food strain .…”

Section: Resultsmentioning

confidence: 99%

“…Thus, the juice yield of dates is very low and the product cost is high. An enzyme cocktail can hydrolyze components of the plant cell wall to facilitate the release of intracellular material with the catalytic conversion of polysaccharides into oligosaccharides and monosaccharides . Moreover, enzyme pretreatment is conducted in mild conditions, which are more eco‐friendly than physical and chemical methods .…”

Section: Introductionmentioning

confidence: 99%

“…An enzyme cocktail can hydrolyze components of the plant cell wall to facilitate the release of intracellular material with the catalytic conversion of polysaccharides into oligosaccharides and monosaccharides . Moreover, enzyme pretreatment is conducted in mild conditions, which are more eco‐friendly than physical and chemical methods . To degrade plant cell walls, the enzyme cocktail generally includes cellulase, hemicellulase, pectinase, etc., because the plant cell wall is a complex polymer composed of cellulose, hemicellulose, lignin, and other components .…”

Section: Introductionmentioning

confidence: 99%

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Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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A low‐cost recycling system for the production of pectinase was built to improve the yield and quality of Muzao date juice and to reduce production costs. An Aspergillus niger strain, Gyx086, was selected from 20 A. niger strains on potato agar plates containing Muzao date residues, and the strain was used to produce an enzyme cocktail with high pectinase activity using Muzao date residues as the medium. The high pectinase activity level was therefore used in the production of Muzao date juice. Under optimized enzymatic hydrolysis conditions, a date juice yield of 92.88% was obtained, which was 11% higher than the control, and was not significantly different from a commercial preparation. Further storage trials indicated that the clarity, viscosity, storage stability, and soluble solid content could also be greatly improved by enzymatic treatment. These results suggested that the cheap experimental preparation could effectively replace the commercial preparation used for the production of date juice. This is the first systematic report detailing the production of Muzao date juice using a cheap and effective pectinase cocktail, which could promote the development of the date juice industry. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology

Han

Chio²,

et al. 2021

Biofuels Bioprod Bioref

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Ginkgo biloba flavonoids are important natural bioactive compounds with strong physiological effects. To develop an eco‐friendly and effective method for extracting flavonoids from plants, a microbial method involving lignocellulose‐degrading bacterial enzymes was developed to improve flavonoid extraction from Ginkgo biloba leaves. In this study, a newly isolated bacterial strain, Paenarthrobacter sp. S1.3, was employed for the fermentation processing of Ginkgo biloba leaf powder. The enzymatic characteristics of xylanase, CMCase, polygalacturonase, and β‐glucosidase from Paenarthrobacter sp. S1.3 were also investigated. The major fermentation parameters for the microbial extraction of Ginkgo biloba flavonoids were optimized using response surface methodology. Under optimal conditions (30.6 °C, initial pH 7.6, and fermentation time 25 h), a total flavonoid yield of 34.15 ± 0.52 mg g−1 dry weight was obtained, which was 2.11 fold and 1.42 fold higher than the yields obtained from the unfermented and ethanol extraction methods, respectively. The optimized results also suggest that the polygalacturonase and β‐glucosidase might play an important role in flavonoid extraction. Fermentation utilizing lignocellulose‐degrading bacteria was a feasible green approach to improve the extraction of natural compounds from plants. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production

Katarina,

Ilić,

Milić

2024

Green Energy and Technology

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Efficient saccharification of agave biomass using Aspergillus niger produced low-cost enzyme cocktail with hyperactive pectinase activity

Cited by 43 publications

References 39 publications

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Extracting flavonoid from Ginkgo biloba using lignocellulolytic bacteria Paenarthrobacter sp. and optimized via response surface methodology

The Realm of Smart Biomass Degrading Enzymes in Low-Carbon Fuels and Chemicals Production

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