Efficient reprogramming of human and mouse primary extra‐embryonic cells to pluripotent stem cells

Nagata, Shogo; Toyoda, Masashi; Yamaguchi, Shinpei; Hirano, Kunio; Makino, Hidenari; Nishino, Koichiro; Miyagawa, Yoshitaka; Okita, Hajime; Kiyokawa, Nobutaka; Nakagawa, Masato; Yamanaka, Shinya; Akutsu, Hidenori; Umezawa, Akihiro; Tada, Takashi

doi:10.1111/j.1365-2443.2009.01356.x

Cited by 82 publications

(73 citation statements)

References 17 publications

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“…Immunocytochemistry-Immunocytochemical analysis was performed as described previously (29,33,38). Human iPSCs were fixed with 4% paraformaldehyde in PBS for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Tateno

Toyota

Saito

et al. 2011

Journal of Biological Chemistry

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191

224

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Induced pluripotent stem cells (iPSCs) can now be produced from various somatic cell (SC) lines by ectopic expression of the four transcription factors. Although the procedure has been demonstrated to induce global change in gene and microRNA expressions and even epigenetic modification, it remains largely unknown how this transcription factor-induced reprogramming affects the total glycan repertoire expressed on the cells. Here we performed a comprehensive glycan analysis using 114 types of human iPSCs generated from five different SCs and compared their glycomes with those of human embryonic stem cells (ESCs; nine cell types) using a high density lectin microarray. In unsupervised cluster analysis of the results obtained by lectin microarray, both undifferentiated iPSCs and ESCs were clustered as one large group. However, they were clearly separated from the group of differentiated SCs, whereas all of the four SCs had apparently distinct glycome profiles from one another, demonstrating that SCs with originally distinct glycan profiles have acquired those similar to ESCs upon induction of pluripotency. Thirty-eight lectins discriminating between SCs and iPSCs/ESCs were statistically selected, and characteristic features of the pluripotent state were then obtained at the level of the cellular glycome. The expression profiles of relevant glycosyltransferase genes agreed well with the results obtained by lectin microarray. Among the 38 lectins, rBC2LCN was found to detect only undifferentiated iPSCs/ESCs and not differentiated SCs. Hence, the high density lectin microarray has proved to be valid for not only comprehensive analysis of glycans but also diagnosis of stem cells under the concept of the cellular glycome.

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“…Immunocytochemistry-Immunocytochemical analysis was performed as described previously (29,33,38). Human iPSCs were fixed with 4% paraformaldehyde in PBS for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Tateno

Toyota

Saito

et al. 2011

Journal of Biological Chemistry

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191

224

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“…These control cells were kindly provided by the National Center for Child Health and Development (Tokyo). Teratoma formation: Teratoma formation was evaluated using a protocol described previously 18) with minor modification. Briefly, clusters of DMD-iPS cells were dispersed by Dispase II (383-02281, Wako Pure Chemical Industries) treatment, collected into tubes, and centrifuged (114 ɡ, 3 minutes).…”

Section: Methodsmentioning

confidence: 99%

Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes

et al. 2016

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SummaryDuchenne muscular dystrophy (DMD) is caused by mutations in the DMD gene which encodes dystrophin protein.Dystrophin defect affects cardiac muscle as well as skeletal muscle. Cardiac dysfunction is observed in all patients with DMD over 18 years of age, but there is no curative treatment for DMD cardiomyopathy. To establish novel experimental platforms which reproduce the cardiac phenotype of DMD patients, here we established iPS cell lines from T lymphocytes donated from two DMD patients, with a protocol using Sendai virus vectors. We successfully conducted the differentiation of the DMD patient-specific iPS cells into beating cardiomyocytes. DMD patient-specific iPS cells and iPS cell-derived cardiomyocytes would be a useful in vitro experimental system with which to investigate DMD cardiomyopathy. ( DMD is caused by mutations in the DMD gene which encodes dystrophin protein. Dystrophin protein constitutes a core element of dystrophin-glycoprotein complex, and plays essential roles in transmitting force and maintaining sarcolemma stability. Lack of dystrophin protein impairs membrane integrity and causes progressive degeneration and loss of skeletal muscle. Dystrophin defect also affects cardiac muscle and cardiac dysfunction is observed in all patients with DMD over 18 years of age.2) With recent advances in the management of respiratory failure for patients with DMD, increasing attention has been paid to cardiac dysfunction since the heart failure is becoming the most frequent cause of death among adult DMD patients.3) Although medical treatments using betablockers and angiotensin converting enzyme inhibitors have been reported to delay progression of DMD cardiomyopathy, 4-6) the effects are limited. Exon skipping has been developed as a novel gene therapy for DMD and some effects have been observed in skeletal muscles, however, the recovery of dystrophin was only scarcely observed in heart muscle due to the difficulty of delivering antisense oligonucleotides to cardiomyocytes.7) Thus, we need to clarify the underlying mechanisms of DMD-associated cardiomyopathy and develop novel therapeutic strategies.To date, mdx mice have been widely used as an animal model of dystrophin gene abnormality. Recent studies have reported various abnormalities in cardiomyocytes of mdx mice such as increased susceptibility to stretch-mediated calcium overload, 8) abnormal activation in stretch-activated channels, 9) diastolic calcium leak from sarcoplasmic reticulum, 10,11) and hyperactivation of X-ROS signaling.12) Nevertheless, mdx mice have critical limitations as a model for DMD cardiomyopathy since they do not develop heart failure. 13,14) On the other hand, using both in vitro and in vivo non-genetic rat models, a recent study demonstrated that microRNA-340-5p may be involved in the progression of heart failure through the interaction with DMD gene.15) Considering these issues, novel experimental platforms that reproduce the cardiac phenotype of DMD patients is critically required for the development of new drugs a...

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“…Although most iPS cells were generated from skin fibroblasts with these reprogramming transcription factors (171,172,211), expression of the same reprogramming factors also appears to initiate a sequence of stochastic events that eventually lead to generating iPS cells in a variety of other differentiated cells, such as neural cells (72,73,154,156), keratinocytes (19), melanocytes (181), adipose-derived cells (164,174), amniotic cells (86,121,216), pancreatic cells (159), and blood cells (50,96,150,161) (Fig. 1).…”

Section: Ectopic Overexpression Of Transcription Factorsmentioning

confidence: 99%

Induced Pluripotent Stem Cells: Emerging Techniques for Nuclear Reprogramming

Han

Yoon

2011

Antioxidants & Redox Signaling

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Introduction of four transcription factors, Oct3/4, Sox2, Klf4, and c-Myc, can successfully reprogram somatic cells into embryonic stem (ES)-like cells. These cells, which are referred to as induced pluripotent stem (iPS) cells, closely resemble embryonic stem cells in genomic, cell biologic, and phenotypic characteristics, and the creation of these special cells was a major triumph in cell biology. In contrast to pluripotent stem cells generated by somatic cell nuclear-transfer (SCNT) or ES cells derived from the inner cell mass (ICM) of the blastocyst, direct reprogramming provides a convenient and reliable means of generating pluripotent stem cells. iPS cells have already shown incredible potential for research and for therapeutic applications in regenerative medicine within just a few years of their discovery. In this review, current techniques of generating iPS cells and mechanisms of nuclear reprogramming are reviewed, and the potential for therapeutic applications is discussed. Antioxid. Redox Signal. 15, 1799-1820.

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Efficient reprogramming of human and mouse primary extra‐embryonic cells to pluripotent stem cells

Cited by 82 publications

References 17 publications

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Glycome Diagnosis of Human Induced Pluripotent Stem Cells Using Lectin Microarray

Generation of Induced Pluripotent Stem Cells From Patients With Duchenne Muscular Dystrophy and Their Induction to Cardiomyocytes

Induced Pluripotent Stem Cells: Emerging Techniques for Nuclear Reprogramming

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