Efficient removal of albumin from human serum by monosize dye-affinity beads

Detection of human proteins using arrayed imaging reflectometry

“…A salient example of this challenge is the detection and quantitation of serum biomarkers of various ailments: for example, cytokines and chemokines (markers of infection and/or inflammation) can have concentration ranges between single pg/mL to hundreds of ng/mL, depending on their role in a specific response pathway (Fenton et al, 1997;Barrios-Rodiles et al, 1999;Ichiyama et al, 2004). In the case of detection from human serum, the technique must also be able to discern the presence of a protein that is in essence a few parts-per-billion "sample impurity" in over 60 mg/mL total protein (Altıntaş and Denizli, 2006). Enzyme-linked immunosorbant assays (ELISAs) are the archetypal biomolecular interaction detection technique, and have been used previously to detect proteins in human serum (Knipping et al, 1995;Boakcle et al, 2006).…”

Interaction of serum albumin with vinyl sulfonate azo dye

“…To separate the albumin, dye-affinity chromatography is commonly used, and Cibacron blue F3G-A (CB) is a ligand of high popularity [2]. In this context, CB is coupled to matrices of various forms, such as microspheres [3][4] and membranes [5][6], or used unbound for albumin extraction with a reversed micellar system [7]. The non-covalent CB-human serum albumin complex has been thoroughly investigated.…”

Hydrophobic Interaction Chromatography: Fundamentals and Applications in Biomedical Engineering

“…Liquid chromatography has the advantage of being easy to use and to scale-up, but are relatively expensive, especially those involving affinity columns. Another drawback of affinity chromatography is the non-specific interactions that lead to the loss of some proteins, with the consequent loss of information (Altintas & Denizli, 2006). In order to overcome the disadvantages of affinity chromatography for its use in blood plasma proteomics, several complementary strategies have been examined, such as sequential anion and cation exchange chromatography followed by 2DGE; and strong cation exchange chromatography followed by liquid-phase isoelectric focusing (Ottens et al, 2005;Barnea et al, 2005).…”