Efficient recovery of cloned human cytomegalovirus DNA fragments from agarose gels

Extraction of Nucleic Acid Fragments from Gels

“…The DNA obtained from this procedure had to be subjected to further cleanup procedures. In another method that combined extrusion with syringe, freeze-thawing the slurry and centrifugation Pramatarova et al reported a recovery of 80% (65).…”

Pellet Pestle Homogenization of Agarose Gel Slices at 45°C for Deoxyribonucleic Acid Extraction

“…Methods which have used elution techniques (6), microconcentrators (7), syringe-based extraction in combination with freeze-thawing (8), nylon membranes (9), centrifugation-based extraction (10 -12), glass beads (13), pumice stones (14), polyester plug spin inserts (15), elution with filtered pipet tips (16), isotachophoresis (17), etc., have been reported.…”