Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for biosensing applications

Mahmoudifard, Matin; Soudi, Sara; Soleimani, Masoud; Hosseinzadeh, Simzar; Esmaeili, Elaheh; Vossoughi, Manouchehr

doi:10.1016/j.msec.2015.09.007

Cited by 48 publications

(32 citation statements)

References 44 publications

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“…It is well known that coupling with EDC/NHS mainly occurred by condensation reaction between the amino groups of the antibody and COOH group on the surface . However, as FTIR results (Figure ) shows there is no carboxyl group (1760–1690 cm −1 ) on the structure of PES nanofibrous membrane.…”

Section: Resultsmentioning

confidence: 97%

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

et al. 2017

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In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency. Therefore, microfluidic immunoassays can be efficiently used as a point-of-care device in medical diagnosis. Surprisingly, we found the increase of specific surface areas of the microfluidic channels improve density of immobilized proteins and leads to higher signal strength. Anti-staphylococcus enterotoxin B (anti-SEB) was used as an analyte model to demonstrate the utility of our proposed platform. Fluorescent microscopy, Fourier transform infrared spectroscopic (FTIR), gas adsorption, contact angle, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), Uv-Vis spectrophotometer and atomic force microscopy (AFM) techniques were used to assess the efficacy of antibody immobilization on the surface. To understand dominant mechanism of protein immobilization, zeta potential measurement was also carried out and it was found electrostatic attraction play significant role in antibody immobilization running into micro- channels containing through EDC/NHS. Moreover, incorporation of nanofibrous membrane causes significant improvement in the signal detection of microfluidic based immunoassay. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1108-1120, 2018.

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Section: Resultsmentioning

confidence: 97%

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

et al. 2017

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“…The degree of leakage in the fabricated microfluidic systems containing plasma and nonplasma‐treated e.NFMs were compared by introducing aqueous solution into the microchannels. In the previous study, we have reported the effect of oxygen plasma treatment on the efficacy of antibody immobilization through EDC/NHS coupling chemistry. Results showed that plasma treatment creates hydrophilic functional groups such as carboxyl and hydroxyl on the surface of substrate, and in these manner, protein capturing on the surface was increase through EDC/NHS coupling chemistry; however, after injecting solution into the microchannels by placing a droplet at the inlet and applying vacuum at the outlet, we observed by naked eye that on the untreated e.NFMs, leakage did not occur, and the fluorescence only appeared in microchannels, which ensured the successful performance of subsequent bioassay procedure carried out within the microfluidic channels.…”

Section: Resultsmentioning

confidence: 99%

“…Most of the high sensitive medical diagnosis systems are designed on the basis of the fact that antibody and antigen interact each other with high specificity. Enzyme‐linked immunosorbent assay (ELISA) using two conjugate antibodies to bind an antigen is the most familiar technique widely applied in medical diagnostics, environmental analyses, and biochemical studies . However, conventional ELISA, which typically carried out in 96‐well microtiter plates, suffer from lacks such as complicated procedures and vigorous washing steps, consumption of large quantities of expensive reagents, and very long assay times .…”

Section: Introductionmentioning

confidence: 99%

Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

Mahmoudifard

Vossoughi

Soleimani

2019

Polymers for Advanced Techs

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Protein capturing on polymeric substrate of microfluidic devices is a key factor for the fabrication of immunoassay with high sensitivity. In this work, simple and versatile technique of electrospinning was used to produce electrospun nanofibrous membranes (e.NFMs) with high surface area as a substrate for microfluidic‐based immunoassay to increase sensitivity. It was found that the simultaneous use of e.NFM and 1‐Ethylethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐Hydroxysuccinimide hydroxysuccinimide as coupling agent has synergic effect on antigen immobilization onto the microchannels. It was found that the oxygen plasma technique for the creation of oxygen containing functional group like carboxyl and hydroxyl causes extreme leakage of solution through the microchannels. Thus, due to capillary effect, it is impossible to use hydrophilic substrate to modify microchannels. In order to compensate this problem, it is propose to utilize other type of polymer for the fabrication of nanofiber to answer this important question that if it is possible to enhance the sensitivity of immunoassay just by changing the polymer type? For this purpose, four different polymers, namely, polycaprolactone, poly lactic‐co‐glycolic acid, poly L‐lactic acid, and polyethersolfone were used as the based material for e.NFM fabrication. Results showed that compared with plain poly (dimethylsiloxane) surface of microchannels, poly lactic‐co‐glycolic acidand poly L‐lactic acid, which inherently contain end‐group of carboxyl in their chemical structure, can improve the protein immobilization, which leads to immunoassay signal enhancement through 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐hydroxysuccinimide coupling chemistry, significantly.

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“…The results obtain from this study shows that PCL nanofibers containing chitosan nanoparticle has a porous structure which help the sustained release of G‐CSF. Protein‐loaded nanofiber scaffolds could be fabricated by several techniques such as, physical absorption, covalent immobilization, emulsion electrospinning, coaxial electrospinning, and suspension electrospinning . Vakilian et al studied the control release of BSA and TGF‐β1 from PCL nanofibers containing protein‐loaded chitosan nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

“…Proteinloaded nanofiber scaffolds could be fabricated by several techniques such as, physical absorption, covalent immobilization, emulsion electrospinning, coaxial electrospinning, and suspension electrospinning. [22][23][24][25][26] Vakilian et al studied the control release of BSA and TGF-b1 from PCL nanofibers containing protein-loaded chitosan nanoparticles. They have reported the sustained release of TGF-b1 and its effect on stem cell differentiation.…”

Section: Discussionmentioning

confidence: 99%

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

Tanha

Rafiee-Tehrani

Abdollahi

et al. 2017

J Biomedical Materials Res

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Sustained release of functional growth factors can be considered as a beneficial methodology for wound healing. In this study, recombinant human granulocyte colony-stimulating factor (G-CSF)-loaded chitosan nanoparticles were incorporated in Poly(ε-caprolactone) (PCL) nanofibers, followed by surface coating with collagen type I. Physical and mechanical properties of the PCL nanofibers containing G-CSF loaded chitosan nanoparticles PCL/NP(G-CSF) and in vivo performance for wound healing were investigated. G-CSF structural stability was evaluated through SDS_PAGE, reversed phase (RP) HPLC and size-exclusion chromatography, as well as circular dichroism. Nanofiber/nanoparticle composite scaffold was demonstrated to have appropriate mechanical properties as a wound dresser and a sustained release of functional G-CSF. The PCL/NP(G-CSF) scaffold showed a suitable proliferation and well-adherent morphology of stem cells. In vivo study and histopathological evaluation outcome revealed that skin regeneration was dramatically accelerated under PCL/NP(G-CSF) as compared with control groups. Superior fibroblast maturation, enhanced collagen deposition and minimum inflammatory cells were also the beneficial properties of PCL/NP(G-CSF) over the commercial dressing. The synergistic effect of extracellular matrix-mimicking nanofibrous membrane and G-CSF could develop a suitable supportive substrate in order to extensive utilization for the healing of skin wounds. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2830-2842, 2017.

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Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for biosensing applications

Cited by 48 publications

References 44 publications

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

Different types of electrospun nanofibers and their effect on microfluidic‐based immunoassay

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

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