Efficient production of transgenic potato (S. tuberosum L. ssp. andigena) plants via Agrobacterium tumefaciens-mediated transformation

Banerjee, Anjan K.; Prat, Salomé; Hannapel, David J.

doi:10.1016/j.plantsci.2005.11.007

Cited by 87 publications

(72 citation statements)

References 27 publications

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“…andigena; Banerjee et al, 2006b). In photoperiodadapted genotypes like andigena, short-day photoperiods (less than 12 h of light) are required for tuber formation, whereas under long-day conditions, no tubers are produced.…”

Section: Plant Materials and Generation Of Transgenic Linesmentioning

confidence: 99%

The Impact of the Long-Distance Transport of aBEL1-Like Messenger RNA on Development

et al. 2012

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BEL1-and KNOTTED1-type proteins are transcription factors from the three-amino-loop-extension superclass that interact in a tandem complex to regulate the expression of target genes. In potato (Solanum tuberosum), StBEL5 and its Knox protein partner regulate tuberization by targeting genes that control growth. RNA movement assays demonstrated that StBEL5 transcripts move through the phloem to stolon tips, the site of tuber induction. StBEL5 messenger RNA originates in the leaf, and its movement to stolons is induced by a short-day photoperiod. Here, we report the movement of StBEL5 RNA to roots correlated with increased growth, changes in morphology, and accumulation of GA2-oxidase1, YUCCA1a, and ISOPENTENYL TRANSFERASE transcripts. Transcription of StBEL5 in leaves is induced by light but insensitive to photoperiod, whereas in stolon tips growing in the dark, promoter activity is enhanced by short days. The heterodimer of StBEL5 and POTH1, a KNOTTED1-type transcription factor, binds to a tandem TTGAC-TTGAC motif that is essential for regulating transcription. The discovery of an inverted tandem motif in the StBEL5 promoter with TTGAC motifs on opposite strands may explain the induction of StBEL5 promoter activity in stolon tips under short days. Using transgenic potato lines, deletion of one of the TTGAC motifs from the StBEL5 promoter results in the reduction of GUS activity in new tubers and roots. Gel-shift assays demonstrate BEL5/POTH1 binding specificity to the motifs present in the StBEL5 promoter and a double tandem motif present in the StGA2-oxidase1 promoter. These results suggest that, in addition to tuberization, the movement of StBEL5 messenger RNA regulates other aspects of vegetative development.

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Section: Plant Materials and Generation Of Transgenic Linesmentioning

confidence: 99%

The Impact of the Long-Distance Transport of aBEL1-Like Messenger RNA on Development

et al. 2012

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“…The pBIB35S-Hyg vector was created by replacing the EcoRI/HindIII cassette of pBIB-HYG (Becker, 1990) with the EcoRI/HindIII cassette of pBINAR (Höfgen and Willmitzer, 1990). pBIB-35S::miR172 was electroporated into Agrobacterium tumefaciens strain C58 GV2260, which was used to transform S. tuberosum subspecies andigena essentially as described (Banerjee et al, 2006a). Hygromycin-B-resistant shoots were regenerated and then propagated from single-node stem cuttings on MS medium containing 20 g l -1 sucrose, 15 mg l -1 hygromycin B, 250 mg l -1 cefotaxime and 2 g l -1 Gelrite (Duchefa Biochemie).…”

Section: Generation Of Plants Overexpressing Mir172mentioning

confidence: 99%

Graft-transmissible induction of potato tuberization by the microRNA miR172

et al. 2009

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The photoreceptor phytochrome B (PHYB) and the homeodomain protein BEL5 are involved in the response of potato tuber induction to the photoperiod. However, whether they act in the same tuberization pathway is unknown. Here we show the effect of a microRNA, miR172, on this developmental event. miR172 levels are higher under tuber-inducing short days than under noninductive long days and are upregulated in stolons at the onset of tuberization. Overexpression of this microRNA in potato promotes flowering, accelerates tuberization under moderately inductive photoperiods and triggers tuber formation under long days. In plants with a reduced abundance of PHYB, which tuberize under long days, both BEL5 mRNA and miR172 levels are reduced in leaves and increased in stolons. This, together with the presence of miR172 in vascular bundles and the graft transmissibility of its effect on tuberization, indicates that either miR172 might be mobile or it regulates long-distance signals to induce tuberization. Consistent with this, plants overexpressing miR172 show increased levels of BEL5 mRNA, which has been reported to be transmissible through grafts. Furthermore, we identify an APETALA2-like mRNA containing a miR172 binding site, which is downregulated in plants overexpressing miR172 and plants in which PHYB is silenced. Altogether, our results suggest that miR172 probably acts downstream of the tuberization repressor PHYB and upstream of the tuberization promoter BEL5 and allow us to propose a model for the control of tuberization by PHYB, miR172 and BEL5.

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“…Potato cv Desirée plants were transformed as previously described by Banerjee et al (2006). Kanamycin-resistant plants were regenerated and grown until tuber development and analyzed for CYP86A33 mRNA accumulation in the tuber skin.…”

Section: Plant Transformation For Rnai-mediated Silencingmentioning

confidence: 99%

CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function

et al. 2008

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Suberin is a cell wall lipid polyester found in the cork cells of the periderm offering protection against dehydration and pathogens. Its biosynthesis and assembly, as well as its contribution to the sealing properties of the periderm, are still poorly understood. Here, we report on the isolation of the coding sequence CYP86A33 and the molecular and physiological function of this gene in potato (Solanum tuberosum) tuber periderm. CYP86A33 was down-regulated in potato plants by RNA interference-mediated silencing. Periderm from CYP86A33-silenced plants revealed a 60% decrease in its aliphatic suberin load and greatly reduced levels of C18:1 v-hydroxyacid (approximately 70%) and a,v-diacid (approximately 90%) monomers in comparison with wild type. Moreover, the glycerol esterified to suberin was reduced by 60% in the silenced plants. The typical regular ultrastructure of suberin, consisting of dark and light lamellae, disappeared and the thickness of the suberin layer was clearly reduced. In addition, the water permeability of the periderm isolated from CYP86A33-silenced lines was 3.5 times higher than that of the wild type. Thus, our data provide convincing evidence for the involvement of v-functional fatty acids in establishing suberin structure and function.

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Efficient production of transgenic potato (S. tuberosum L. ssp. andigena) plants via Agrobacterium tumefaciens-mediated transformation

Cited by 87 publications

References 27 publications

The Impact of the Long-Distance Transport of aBEL1-Like Messenger RNA on Development

The Impact of the Long-Distance Transport of aBEL1-Like Messenger RNA on Development

Graft-transmissible induction of potato tuberization by the microRNA miR172

CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function

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