Efficient production of transgenic melon via Agrobacterium-mediated transformation

Bezirganoğlu, İsmail; Hwang, Shih Ying; Shaw, Jei‐Fu; Fang, Tony J.

doi:10.4238/2014.april.25.7

Cited by 12 publications

(7 citation statements)

References 21 publications

(23 reference statements)

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“…by using co-cultivation with Agrobacterium (Table 3). Some improved protocols have been lately reported in melon (Bezirganoglu et al 2014), cucumber (Wang et al 2015) and watermelon (Liu et al 2016). In the cucurbitaceous species, an efficient regeneration procedure via somatic embryogenesis from embryogenic calli, developed from leaf explants, has also been recently published (Vinoth & Ravindhran, 2015).…”

Section: Genetic Engineering For Fruit Quality and Yield Enhancementmentioning

confidence: 99%

Genomic and biotechnological interventions for crop improvement in cucurbitaceous crops: A review

Ram¹,

Berwal²,

Gora³

et al. 2019

JAE

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The cucurbitaceous family has comprised with diverse economically important cucurbits. It primarily comprised of 118 genera and 825 species which being consumed as food worldwide since the domestication of the plants. In India, cucurbits are being grown throughout regions of the country including hot semiarid and arid zones. With the advent of genomic breakthrough, a large number of genomic and biotechnological interventions have been developed in cucurbitaceous crops. The plenty of molecular markers are available in cucurbits and these markers were deployed to assess the genetic diversity and mapping of the QTLS/genes of interest. The success in development of genomic tools may happens by genome sequencing of mostly important cucurbitaceous crops such as watermelon, cucumber, muskmelon, bottle gourd, pumpkins. Transgenic and non-transgenic plants were developed in various cucurbitaceous crops by employing of Agrobacterium-mediated transformation and CRISPR/CAS9 approach, respectively. Thus cucurbitaceous crops have been considerably exploited at molecular level and biotechnological interventions were developed for crop improvement. However, a comprehensive report in cucurbitaceous crops regarding genomic and biotechnological developments is not available in public domain. Therefore, in the present review, we have collected the information related to genomics and biotechnology in cucurbits and emphasized on some successful interventions.

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Section: Genetic Engineering For Fruit Quality and Yield Enhancementmentioning

confidence: 99%

Genomic and biotechnological interventions for crop improvement in cucurbitaceous crops: A review

Ram¹,

Berwal²,

Gora³

et al. 2019

JAE

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“…Various (young) explants have been generally inoculated with Agrobacterium followed by regeneration through shoot organogenesis, but transformation efficiency varied largely with the genotype used ( Klocke et al, 2010 ; Manamohan et al, 2011 ). Some improvements in the protocol have been lately reported for melon ( Bezirganoglu et al, 2014 ; Zhang et al, 2014 ), cucumber ( Wang et al, 2015 ), Cucurbita spp. ( Nanasato et al, 2013 ), and watermelon ( Liu et al, 2016 ).…”

Section: Regeneration and Transformationmentioning

confidence: 99%

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

2017

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In the frame of modern agriculture facing the predicted increase of population and general environmental changes, the securement of high quality food remains a major challenge to deal with. Vegetable crops include a large number of species, characterized by multiple geographical origins, large genetic variability and diverse reproductive features. Due to their nutritional value, they have an important place in human diet. In recent years, many crop genomes have been sequenced permitting the identification of genes and superior alleles associated with desirable traits. Furthermore, innovative biotechnological approaches allow to take a step forward towards the development of new improved cultivars harboring precise genome modifications. Sequence-based knowledge coupled with advanced biotechnologies is supporting the widespread application of new plant breeding techniques to enhance the success in modification and transfer of useful alleles into target varieties. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 system, zinc-finger nucleases, and transcription activator-like effector nucleases represent the main methods available for plant genome engineering through targeted modifications. Such technologies, however, require efficient transformation protocols as well as extensive genomic resources and accurate knowledge before they can be efficiently exploited in practical breeding programs. In this review, we revise the state of the art in relation to availability of such scientific and technological resources in various groups of vegetables, describe genome editing results obtained so far and discuss the implications for future applications.

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“…DNA content and changes in chromosome number have been reported in several transgenic melon subspecies through ow cytometric analysis. In most reports concerning melon transformation, the ploidy levels of the transgenic lines were not speci ed (Switzenberg et al, 2014;Bezirganoglu et al, 2014) and in some cases a high percentage of instability was observed. In one of the latest melon transformation expriments (GarciaAlmodovar et al, 2017), a large number of transgenic plants became tetraploid, whereas the diploid level decreased signi cantly in tranformed melons when the infected explants grew from seedling.…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability in Melon Transformation System: Assessment by Flow Cytometry and ISSR Markers

Raji

Farajpour

2021

Preprint

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Genetic instability in melon species sometimes occurs as a result of in vitro tissue culture and transformation systems. This study describes a new regeneration technique for agrobacterium-mediated co-culture of muskmelon explants (Cucumis melo L. c.v. ‘Khatooni’). Here, no genetic instability was observed in positive PCR regenerants. 4-day-old cotyledonary explants had been infected with LBA4404 Agrobacterium suspensions. The co-cultivation occurred in the presence of 100mg/l rifampicin and 50mg/l kanamycin. The bacteria contained a binary vector pBI121 carrying nopaline synthase by the promoter-neomycin phosphotransferase gene. The regeneration succeeded 65% in selective MS medium containing N6-benzylaminopurine (600 µg/l), β-naphthoxyacetic acid (25 µg/l) and 50mg/l kanamycin in inoculated 4-day-old cotyledonary explants. According to the polymerase chain reaction analysis of neomycin phosphotransferase II gene, transformation was merely successful (8.4%), indicating a substantial miss on a large number of regenerated plants in the selective medium, as a consequence of PGR and antibiotic imbalances. Inter Single Sequence Repeat markers and flow cytometry analyses were used for evaluating the genetic stability and ploidy level of transplants, respectively. The integrated approach underlined that Agrobacterium inoculation and plant growth regulators were successfully combined in vitro to enable muskmelon transformation.

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Efficient production of transgenic melon via Agrobacterium-mediated transformation

Cited by 12 publications

References 21 publications

Genomic and biotechnological interventions for crop improvement in cucurbitaceous crops: A review

Genomic and biotechnological interventions for crop improvement in cucurbitaceous crops: A review

Genetic Transformation and Genomic Resources for Next-Generation Precise Genome Engineering in Vegetable Crops

Genetic Stability in Melon Transformation System: Assessment by Flow Cytometry and ISSR Markers

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