Efficient production of reuterin from glycerol by magnetically immobilized Lactobacillus reuteri

Abstract

Reuterin is a compound that contains the monomeric, hydrated monomeric, and cyclic dimeric forms of 3-hydroxypropionaldehyde (3-HPA). It is widely used as a food preservative due to its antimicrobial properties, and 3-HPA is also an important precursor for several industrial chemicals. In this study, we first developed an efficient immobilization process involving magnetic cell entrapment for production of reuterin from glycerol by Lactobacillus reuteri. The cell growth condition was optimized by statistical approaches. High conversion efficiency was achieved using k-carrageenan as the immobilization support and Fe3O4 as magnetic nanoparticles. Furthermore, addition of 0.02 g/L vitamin B12, 10 mmol/L Mg(2+), and glucose in a molar ratio of 0.15 to glycerol significantly increased the conversion rate. Under optimal conditions, 235.9 mmol/L 3-HPA was produced from 250 mmol/L initial glycerol in 1.5 h, with a molar yield of 94.4 % and a productivity of 15.4 mmol/(g dry cell weight∙L∙h), which were the highest values to date. Thus, this study demonstrated a promising process for the improvement of biocatalyst efficiency in biotransformation.

CRISPR-Cas9/Cas12a biotechnology and application in bacteria

“…LAB possess specific characteristics, including small genome sizes, high sugar uptake, high tolerance to environmental stress, and uncoupled growth and energy metabolism. Such features render them natural cell factories for industrial production of metabolites and enzymes [ [182] , [183] , [184] , [185] ].…”

Efficient biodegradation of chlorophenols in aqueous phase by magnetically immobilized aniline-degrading Rhodococcus rhodochrous strain

“…With the increase of Fe 3 O 4 NPs, the biodegradation rate by immobilized cells was increased accordingly till to the Fe 3 O 4 NPs concentration of 9 g/L. These results revealed that the biodegradation activity of the immobilized R. rhodochrous DSM6263 cells was significantly enhanced by adding Fe 3 O 4 NPs, which should be beneficial to the reduced mass transfer resistance and the accelerated material transportation as compared to the traditional immobilization methods [ 16 ].…”

“…For preparation of magnetically immobilized cells, Fe 3 O 4 nanoparticles (NPs) suspension was added to the above dissolved k -carrageenan with the same amount of cell suspension, and solidified in the same procedure [ 13 ]. The Fe 3 O 4 NPs were prepared by the modified coprecipitation method as previous report [ 16 ].…”

“…Nanoparticles (NPs) represent a new generation of environmental remediation technologies that could provide cost-effective solutions to some of the most challenging environmental clean-up problems [ 12 ]. The magnetic materials are widely used in various fields, such as drug delivery and biosensing, environmental remediation and biotechnological production process [ 13 – 16 ]. Therefore, the exploitation of nanoscale technology in environmental applications appears very promising.…”

Untitled

“…It is an intermediate in the metabolism of glycerol to 1,3-propanediol catalyzed by the coenzyme B12-dependent diol dehydrase (oxygen-sensitive and membrane-associated glycerol dehydratase). If not released, 3-HPA is reduced by the (NADH)+H+-dependent-1,3-propanediol-oxydoreductase regenerating NAD+ [21][22][23]. The production is rate-limited because, if overproduced, reuterin is toxic for the producer cell [21].…”