Efficient production of fatty acid methyl esters by a wastewater-isolated microalgae-yeast co-culture

Suastes-Rivas, Jessica K.; Hernández‐Altamirano, Raúl; Mena‐Cervantes, Violeta Y.; Valdez-Ojeda, Ruby; Toledano-Thompson, Tanit; Tovar-Gálvez, Luis R.; López-Adrián, Silvia; Chairez, Isaac

doi:10.1007/s11356-019-07286-1

Cited by 14 publications

(4 citation statements)

References 57 publications

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“…A consortium of yeast composed of Saccharomyces and Candida has been used to produce multiple enzymes [ 58 ]. A microalgae consortium with Candida yeast isolated from wastewater were tested to increase fatty acids in biodiesel production [ 59 ], as well as bioethanol production with a consortium of Candida and Hanseniaspora yeasts [ 60 ] and oil production with a consortium of the microalgae Chlamydomonas and the oil yeast Lipomyces [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

Towards the Development of Microbial Ecotoxicology Testing Using Chlorpyrifos Contaminated Sediments and Marine Yeast Isolates as a Model

et al. 2022

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Chlorpyrifos (CP), a widely used pesticide, and its metabolite 3,5,6-trichloro-2-pyridinol (3,5,6-TCP), are xenobiotic compounds detected in many biomes, notably in marine sediments, all over the world. These compounds are posing a serious environmental and health problem given their toxicity to wildlife and possible exposure effects to human neurodevelopment. Microorganisms at CP-impacted environments could harbor metabolic capabilities that can be used as indicators of the biological effects of the contaminant and could encode selected functions reactive against contaminants. Those features could be used for microbial ecotoxicology applications by collectively using analytical, enzymatic, microbiological and toxicological techniques in order to assess the biological effects of pollutants and other environmental/climatic stressors in ecosystems. The objective of this study was to assess the variability in the metabolic responses of yeast isolates from CP-contaminated marine sediments as potential biological indicators for microbial ecotoxicology testing. Sediment samples from a South Caribbean tropical shore (Cartagena Bay, Colombia) were collected, and deoxyribonucleic acid (DNA) was recovered from lyophilized aliquots. The DGGE (Denaturing Gradient Gel Electrophoresis) technique targeting fungal Internal Transcribed Spacer (ITS) showed the great diversity of fungal types. Simultaneously, yeast strains were isolated from the freshly collected sediment samples. Physiological characterization including API 20C and antibiosis tests, growth patterns at salt concentrations (2/4/10/25%), temperatures (4/25/37/45 °C), esterase activity assay and resistance tests to CP/TCP toxicity resulted in 10 isolated yeast strains, identified as Candida spp. (6), Cryptococcus spp. (3). and Rhodotorula spp. (1), showing promising characteristics to be used as a test for yeast-based ecotoxicity indicators. The patterns of carbohydrate assimilation, low antibiosis, presence of esterases/lipases, growth in a wide range of temperatures and salt concentrations, and tolerance to minimal inhibitory concentrations of CP and TCP are factors useful for testing environmental samples.

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Section: Resultsmentioning

confidence: 99%

Towards the Development of Microbial Ecotoxicology Testing Using Chlorpyrifos Contaminated Sediments and Marine Yeast Isolates as a Model

et al. 2022

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“…Recently, microbial co-culture system has been widely used to synthesize complex structure chemicals, such as plant derived natural products [ 33 , 34 ]. In terms of short-chain fatty acid esters, microbial co-culture system shows advantages in the elimination of alcohol or acid supplementation [ 1 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation for the one-pot biosynthesis of butyl acetate by using microbial mono- and co-cultures

Jiang

et al. 2021

Biotechnol Biofuels

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Background Butyl acetate has shown wide applications in food, cosmetics, medicine, and biofuel sectors. These short-chain fatty acid esters can be produced by either chemical or biological synthetic process with corresponding alcohols and acids. Currently, biosynthesis of short chain fatty acid esters, such as butyl butyrate, through microbial fermentation systems has been achieved; however, few studies regarding biosynthesis of butyl acetate were reported. Results In this study, three proof-of-principle strategies for the one-pot butyl acetate production from glucose through microbial fermentation were designed and evaluated. (1) 7.3 g/L of butyl acetate was synthesized by butanol-producing Clostridium acetobutylicum NJ4 with the supplementation of exogenous acetic acid; (2) With the addition of butanol, 5.76 g/L of butyl acetate can be synthesized by acetate-producing Actinobacillus succinogenes130z (ΔpflA); (3) Microbial co-culture of C. acetobutylicum NJ4 and A. succinogenes130z (ΔpflA) can directly produce 2.2 g/L of butyl acetate from glucose by using microbial co-culture system with the elimination of precursors. Through the further immobilization of A. succinogenes130z (ΔpflA), butyl acetate production was improved to 2.86 g/L. Conclusion Different microbial mono- and co-culture systems for butyl acetate biosynthesis were successfully constructed. These strategies may be extended to the biosynthesis of a wide range of esters, especially to some longer chain ones.

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“…One such system has already been reported, a Synechococcus elongates-Pseudomonas putida consortium was constructed to produce bioplastic (PHA, polyhydroxyakanotate) (García-Jiménez et al, 2018). Another microalgae-yeast co-culture was isolated from wastewater and identified to contain a number of microalgae and yeast species, which was also successfully used for fatty acid methyl esters production (Suastes-Rivas et al, 2019). From our perspective, these autotroph-heterotroph communities have the potential to produce specific FACs from CO 2 .…”

Section: Microbial Chassis For Biosynthesis Of Facsmentioning

confidence: 99%

Opportunities and Challenges for Microbial Synthesis of Fatty Acid-Derived Chemicals (FACs)

Liu

Benitez

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Global warming and uneven distribution of fossil fuels worldwide concerns have spurred the development of alternative, renewable, sustainable, and environmentally friendly resources. From an engineering perspective, biosynthesis of fatty acid-derived chemicals (FACs) is an attractive and promising solution to produce chemicals from abundant renewable feedstocks and carbon dioxide in microbial chassis. However, several factors limit the viability of this process. This review first summarizes the types of FACs and their widely applications. Next, we take a deep look into the microbial platform to produce FACs, give an outlook for the platform development. Then we discuss the bottlenecks in metabolic pathways and supply possible solutions correspondingly. Finally, we highlight the most recent advances in the fast-growing model-based strain design for FACs biosynthesis.

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Efficient production of fatty acid methyl esters by a wastewater-isolated microalgae-yeast co-culture

Cited by 14 publications

References 57 publications

Towards the Development of Microbial Ecotoxicology Testing Using Chlorpyrifos Contaminated Sediments and Marine Yeast Isolates as a Model

Towards the Development of Microbial Ecotoxicology Testing Using Chlorpyrifos Contaminated Sediments and Marine Yeast Isolates as a Model

Comprehensive evaluation for the one-pot biosynthesis of butyl acetate by using microbial mono- and co-cultures

Opportunities and Challenges for Microbial Synthesis of Fatty Acid-Derived Chemicals (FACs)

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