Efficient production of celery embryos and plantlets released in culture of immobilized gel beads

Suehara, Ken Ichiro; Kohketsu, Katsuhito; Uozumi, Nobuyuki; Kobayashi, Takeshi

doi:10.1016/0922-338x(95)94752-d

Cited by 15 publications

(1 citation statement)

References 4 publications

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“…Morris and Fowler (1981) reported a method for producing the fine (almost single) plant cell suspension based on leakage of cells from immobilized system. Suehara et al (1995) reported that celery embryos and plantlets were selectively released in a culture of immobilized Ca-alginate gel beads in which celery callus was entrapped under regeneration conditions.…”

Section: Resultsmentioning

confidence: 99%

Development of a quantitative method for determination of the optimal conditions for protoplast isolation from cultured plant cells

Aoyagi

2006

Biotechnol Lett

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An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the basis of the kv [31.7 x 10(3) (number/ml min)]. The colony-forming efficiency of the protoplasts was about 46%. The optimal conditions for protoplasts isolation from Catharanthus roseus [kv = 38.1 x 10(3) (number/ml min)] and Wasabia japonica [kv = 14.2 x 10(3) (number/ml min)] cultured cells could also be determined. Furthermore, a method for rapid regenerating cell wall of protoplast in liquid culture using alginate gel containing locust bean gum was developed.

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Section: Resultsmentioning

confidence: 99%