Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

Li, Yonghao; Zhang, Peng; Zhu, Deying; Yao, Bo; Hasunuma, Tomohisa; Kondo, Akihiko; Zhao, Xin‐Qing

doi:10.1016/j.bej.2021.108296

Cited by 14 publications

(7 citation statements)

References 43 publications

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“…41 The results showed that MGS, a novel inducer consisting of a sugar mixture, can be used successfully as an inducer in cellulase production; in large-scale processing, MGS would be a better inducer than MGD, and the preparation process would be simpler and more economical. 10,41…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study also showed that the strain could produce cellulase in the presence of glucose. 41 …”

Section: Resultsmentioning

confidence: 99%

“…This indicated that sophorose in MGS also promoted the transcription of the cellulase gene at a trace concentration, which was equivalent to the b-disaccharide induction level achieved with MGD. 41 The results showed that MGS, a novel inducer consisting of a sugar mixture, can be used successfully as an inducer in cellulase production; in large-scale processing, MGS would be a better inducer than MGD, and the preparation process would be simpler and more economical. 10,41 Since there were large amounts of glucose in MGS, glucose was used as a negative control, and MGD was used as a positive control to identify the ability to induce cellulase production.…”

Section: Cellulase Production By T Reesei Rut C30 Using Different Ind...mentioning

confidence: 99%

“…Our previous study also showed that the strain could produce cellulase in the presence of glucose. 41 Determination of the bioactivity of isosteviol Fig. 1 shows the inhibitory effects of isolated and puried isosteviol on A. niger, S. cerevisiae and E. coli.…”

Section: Preparation and Analysis Of Sophorose And Isosteviolmentioning

confidence: 99%

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Induction of cellulase production in Trichoderma reesei by a glucose–sophorose mixture as an inducer prepared using stevioside

Zhang

Chen

et al. 2022

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

“…Our previous study also showed that the strain could produce cellulase in the presence of glucose. 41 …”

Section: Resultsmentioning

confidence: 99%

Section: Cellulase Production By T Reesei Rut C30 Using Different Ind...mentioning

confidence: 99%

Section: Preparation and Analysis Of Sophorose And Isosteviolmentioning

confidence: 99%

See 2 more Smart Citations

Induction of cellulase production in Trichoderma reesei by a glucose–sophorose mixture as an inducer prepared using stevioside

Zhang

Chen

et al. 2022

RSC Adv.

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“…In contrast, lower concentrations (1%) of cellulases acted as positive reinforcement for cellulase production and biomass growth in the later stages of cell growth after the exhaustion of simple carbon sources (Ma et al, 2013). Also, in terms of complex nitrogen source, corn steep liquor (CSL) has been reported to strongly support high cellulase production in Trichoderma reesei which is why it was incorporated in the production media (Farid and El-Shahed, 1993;Li et al, 2022). maintained in Potato Dextrose agar (PDA) (Merck, Germany) slants at -4ºC.…”

Section: Resultsmentioning

confidence: 99%

A study on critical associations of media components on enhanced cellulase production from wild Trichoderma viride and cellulase immobilization on iron-oxide magnetic nanoparticles

Revathi¹,

Ramalingam²

2023

JEB

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Aim: The current study is a preliminary step towards enhancing the cellulase productivity in wild Trichoderma viride which will enable robust valorization of non-edible lignocellulosic biomass through co-generative enzymatic saccharification, specifically concentrating on influence of individual media components on biomass growth and cellulase productivity. Further, cellulase immobilization on iron-oxide magnetic nanoparticles was also achieved that can increase the shelf life of the enzyme. Methodology: The cellulase production in the wild Trichoderma viride was enhanced using media design and formulation. EDC {1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide} functionalized iron-oxide nanoparticles were chosen to act as carriers for cellulase immobilization. The binding efficiency and relative activity were measured in addition to optimal pH and temperature for cellulase bound iron-oxide nanoparticles. Further, the hydrolysis efficiency of immobilized cellulases was also measured after which it was subjected to consecutive hydrolytic cycles to calculate the recycle rate. Results: A maximum growth rate of 60 PCV (Packed cell volume) and total cellulase activity of 7.4 U ml-1 was obtained on media design and formulation. 82.5% binding efficiency was achieved on EDC functionalized iron-oxide magnetic nanoparticles which showed good stability at 5pH and 500C. There was 44.4% activity loss after 5 consecutive hydrolytic cycles which showed steady decline with increased cycle number and finally at the end of the 10th hydrolytic cycle, 22.2% of total relative activity was retained. Interpretation: Unprecedented total cellulase activity from a wild strain was obtained through media design. The stability of cellulases was further enhanced using iron-oxide magnetic nanoparticle immobilization. Key words: Cellulases, Immobilization, Iron-oxide magnetic nanoparticles, Submerged fermentation, Trichoderma viride

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A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

Gao,

Xu,

Tian

et al. 2024

eFood

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The presence of stone cells in pears is recognized as a problem for the pear processing industry. Bacillus and mold can degrade stone cells because of their potential to digest cellulose and lignin, but they cannot be used for the degradation test of pear stone cells. In this study Lactoplantibacillus plantarum JYLP‐326 was used in single culture or in the mixed culture with Saccharomyces cerevisiae Y2 to study their potential to degrade stone cells during fermentation and the mechanism of degradation was further explored. Synergy in cellulase activity was observed in the mixed‐culture where the maximum activity was observed at 96th hour of fermentation. Activities of endoglucanase, exoglucanase and β‐glucosidase were 1.75, 4.58, and 2.31. The degradation rate of stone cells in the mixed‐culture was 37.67%, which was significantly higher than that the results obtained for single cultures. The results of scanning electron microscopy (SEM) showed that the surface of the cultured stone cells became rough. Metabolomics studies confirmed the presence of specific metabolites related to the degradation of stone cells after the fermentation. It was concluded that the mixed‐culture fermentation using the above‐mentioned strains could be exploited by the pear processing industry to degrade stone cells.

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Efficient preparation of soluble inducer for cellulase production and saccharification of corn stover using in-house generated crude enzymes

Cited by 14 publications

References 43 publications

Induction of cellulase production in Trichoderma reesei by a glucose–sophorose mixture as an inducer prepared using stevioside

Induction of cellulase production in Trichoderma reesei by a glucose–sophorose mixture as an inducer prepared using stevioside

A study on critical associations of media components on enhanced cellulase production from wild Trichoderma viride and cellulase immobilization on iron-oxide magnetic nanoparticles

A study revealing mechanisms behind the stone cell of Yali pear degradation by mixed‐culture fermentation of lactic acid bacteria and yeast

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