Efficient Preparation of Liposomes Encapsulating Food Materials Using Lecithins by a Mechanochemical Method

Takahashi, Makoto; Inafuku, Kei-ichiro; Miyagi, T.; Oku, Hirosuke; Wada, Kōji; Imura, Tomohiro; Kitamoto, Daï

doi:10.5650/jos.56.35

Cited by 84 publications

(42 citation statements)

References 28 publications

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“…16) Liposomes encapsulating the ukon extract including curcumin were obtained by this method which applied homogenization and microfluidization. The preparation of liposomes containing the extract (LUE) was further investigated in this study in order to obtain the optimum conditions leading to a stable and functional liposomal carrier.…”

Section: Resultsmentioning

confidence: 99%

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Characterization and Bioavailability of Liposomes Containing a Ukon Extract

Takahashi

Kitamoto

Imura

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Section: Resultsmentioning

confidence: 99%

“…We have previously reported a new mechanochemical method 16) for the large-scale preparation of liposomes using a homogenizer and microfluidizer. This mechanochemical method allowed us to prepare liposomes containing a ukon extract by using commercial soybean lecithins.…”

Section: )mentioning

confidence: 99%

Characterization and Bioavailability of Liposomes Containing a Ukon Extract

Takahashi

Kitamoto

Imura

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…With this technology solids, liquids or gaseous materials are packed into miniature capsules (membranes) that can subsequently release their contents at controlled rates under particular circumstances. Indeed, based on their ability to act as micro-or nanocarrier systems for the secure delivery of bioactive agents, liposomes are undergoing extensive research and development in, amongst others, the pharmaceutical, cosmetic and food industries [6,7]. Liposomes, reportedly first described in the mid-1960s [8], are spherical (although shape is influenced by the structure of the different components) particles consisting of a membranous system formed by single (unilamellar) or multiple (multilamellar) lipid bilayers, resembling the lipid membrane of cells [9], which can be either small or large ( Figure 1).…”

Section: Liposomal Technologymentioning

confidence: 99%

Liposomal Nanotechnology - A New Frontier for Sport and Exercise Nutrition?

Higgins¹

2016

JNMR

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“…We previously reported that highly stable liposomes encapsulating the extract of Ukon are efficiently obtained by the mechanochemical method using a homogenizer and microfluidizer 23) . In order to improve the dispersibility and stability of liposomes encapsulating AGE, the liposomes were thus prepared by the new method with SLP-WHITE of 1 wt% and 0.25 or 0.5 wt% of AGE.…”

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confidence: 99%

“…We previously demonstrated that a commercially available lecithin, SLP-WHITE, self-assembles to form liposomes, which can efficiently encapsulate functional food materials 23) . We thus prepared liposomes from SLP-WHITE (1 wt%) and AGE by the Bangham method, and checked the fundamental interfacial properties.…”

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confidence: 99%

Liposomes Encapsulating Aloe vera Leaf Gel Extract Significantly Enhance Proliferation and Collagen Synthesis in Human Skin Cell Lines

Takahashi

Kitamoto²,

Asikin

et al. 2009

J. Oleo Sci.

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Aloe vela leaf gel extract (AGE) are widely used as cosmetic and pharmaceutical ingredients because of its versatile skin care properties. In order to enhance the bioavailability of AGE, liposomes encapsulating AGE were prepared and examined for their interfacial and biochemical properties. The liposomes prepared from a soybean lecithin (SLP-WHITE, 1.0 wt%) by the Bangham method gave relatively a good trapping efficiency up to the AGE concentration of 0.5 wt%. The stable liposomes were then prepared from 1.0 wt% of SLP-WHITE and different concentrations of AGE by the mechanochemical method using a homogenizer and microfluidizer. The liposomes obtained from 0.25 wt% of AGE were confirmed to be small unilamellar vesicles with a diameter of less than 200 nm, and remained well dispersed for at least two weeks. The obtained liposomes encapsulating AGE were further examined for the effects on proliferation and type I collagen synthesis in normal human neonatal skin fibroblasts, NB1RGB cells. Liposomal AGE clearly showed higher proliferation rate than that of AGE alone. In addition, compared to the control, liposomal AGE significantly increased the collagen synthesis by 23%, while AGE alone showed a small effect. Liposomal AGE was also assayed for the effect on proliferation in normal human epidermal keratinocytes, NHEK(F) cells. Interestingly, liposomal AGE fractions containing 4 and 20 mg/mL of the extract considerably increased the proliferation rate by 77% and 101%, respectively. In contrast, AGE alone fractions containing 4 and 20 mg/mL of the extract increased the rate by 41% and 60%, respectively. Accordingly, the bioavailability and skin care properties of AGE will be significantly enhanced by liposome encapsulation, and the present liposomal AGE should have a great potential as an effective skin care formulation.

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Efficient Preparation of Liposomes Encapsulating Food Materials Using Lecithins by a Mechanochemical Method

Cited by 84 publications

References 28 publications

Characterization and Bioavailability of Liposomes Containing a Ukon Extract

Characterization and Bioavailability of Liposomes Containing a Ukon Extract

Liposomal Nanotechnology - A New Frontier for Sport and Exercise Nutrition?

Liposomes Encapsulating Aloe vera Leaf Gel Extract Significantly Enhance Proliferation and Collagen Synthesis in Human Skin Cell Lines

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