Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate

Abstract

The present study focused on the screening and optimization of biopolymer polyhydroxybutyrate (PHB) production by Bacillus spp. using cost-effective substrates. Among 602 local Bacillus isolates, Bacillus thuringiensis B417-5 produced the highest amount of PHB (2.278 g/L, 60.07% of dry cell weight, DCW). 1 H NMR and FTIR analyses of the extracted polymer revealed the characteristic peaks of PHB. The optimization results showed that the highest PHB accumulation (2.768 g/L, 72.08% of DCW) was achieved when culturing B. thuringiensis B417-5 in a nitrogen-deficient medium containing 1% total sugar from sugarcane juice and 0.5% yeast extract, with a pH of 7.0 and an incubation temperature of 37 °C for 48 h. B. thuringiensis B417-5 can thus be considered a good candidate for large-scale production of PHB. We are reporting for the first time that sugarcane juice is a promising carbon source for economical PHB production by B. thuringiensis.

Isolation of Two Bacterial Species from Argan Soil in Morocco Associated with Polyhydroxybutyrate (PHB) Accumulation: Current Potential and Future Prospects for the Bio-Based Polymer Production

“…It is reported in the literature that over 90 bacterial genera have been identified as PHB producers. Among all the isolated and identified species, many colonize soil environments, and few have been isolated from waste water, sludge ecosystems, industrial wastes, and animal manure [ 9 ].…”

“…Due to all these good mechanical and thermal properties, PHB captured the attention of industrial productions [ 8 ]. PHBs have been used mainly for packaging, in agriculture, food industry, and also in medical and pharmaceutical fields [ 9 ]. Moreover, PHB can be degraded, aerobically and anaerobically, by a biodegradation process [ 2 ].…”

“…Thammasittirong et al [ 7 ] reported that not only the species and the substrate used can influence the quality of PHB synthesized, but also the culture optimization parameters, such as nutrients and their proportions, pH, and temperature. They reported how the Bacillus thuringiensis , strain B417-5, exhibits high efficiency production on a large scale using sugarcane, at a neutral pH, incubating for 48 h at 37 °C and shaking at 100 rpm [ 9 ]. The 70 to 80% of the PHB production costs account for the carbon sources, which is why it is important to adopt wastes rich in carbon to afford the large-scale production of this polymer.…”

Screening of PHB biopolymer producing Bacillus cereus from municipal sludge waste

“…Briefly the dilutions were inoculated 5% w/v on selective media such as Bacillus cereus selective agar base enriched with Disodium hydrogen phosphate (2.5g) and Pottasium dihydrogen phosphate (0.25g), Peptone (1g), Mannitol (10g), Sodium chloride (2g), Megnesium sulphate (0.1g), Sodium pyruvate (10g), Bromothymol blue (0.12g) and agar (2g) and incubated at 30 0 C for 24 h in an shaker incubator. Plates were incubated at 30 o C for 2-3 days, and colonies appeared and was exposed to visible light and those showing slight bluish colonies were earmarked as presumptive PHB producers (Thammasittirong et al, 2017).…”

Biodegradable Plastic (Phb) From Bacteria Collected From Gopalpur Beach, Bhubaneswar

“…There is a need to study and to develop new biodegradable polymers with plastic-like properties [1,2]. Polyhydroxybutyrate (PHB) is a linear polymer of D-3-hydroxyl butyrate and best known of the polymers related to polyhydroxyalkanoates that is produced by several microorganisms as an energy source [3,4]. Biodegradable plastics offer 'eco-friendly' perspectives in nature, protecting the environment from hazards caused by conventional petroleum-based plastics [5].…”