Efficient polyhydroxyalkanoate (PHA) accumulation by a new continuous feeding mode in three-stage mixed microbial culture (MMC) PHA production process

Chen, Zhiqiang; Huang, Long; Wen, Quan; Guo, Zirui

doi:10.1016/j.jbiotec.2015.06.382

Cited by 41 publications

(22 citation statements)

References 25 publications

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“…At the moment, we are witnessing increasing efforts to optimize the application of mixed microbial consortia (MMC) for PHA biosynthesis as an inexpensive alternative to processes based on pure, monoseptic cultures. For such MMC-based processes, selective environmental pressure is exerted to a natural microbial consortium by employing suitable cultivation conditions [64][65][66][67][68][69][70][71]. As PHA are storage polymers naturally accumulated under dynamic environmental conditions, selective pressure to boost PHA biosynthesis can be achieved via so-called "cyclic feast-and-famine feeding regimes", which consist of a series of alternating availability (feast) and absence of nutrients (famine) [71,72].…”

Section: Batch Systemsmentioning

confidence: 99%

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Koller

2018

Fermentation

145

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Polyhydroxyalkanoates (PHA) are microbial biopolyesters utilized as "green plastics". Their production under controlled conditions resorts to bioreactors operated in different modes. Because PHA biosynthesis constitutes a multiphase process, both feeding strategy and bioreactor operation mode need smart adaptation. Traditional PHA production setups based on batch, repeated batch, fed-batch or cyclic fed-batch processes are often limited in productivity, or display insufficient controllability of polyester composition. For highly diluted substrate streams like is the case of (agro) industrial waste streams, fed-batch enhanced by cell recycling has recently been reported as a viable tool to increase volumetric productivity. As an emerging trend, continuous fermentation processes in single-, two-and multi-stage setups are reported, which bring the kinetics of both microbial growth and PHA accumulation into agreement with process engineering and allow tailoring PHA's molecular structure. Moreover, we currently witness an increasing number of CO 2-based PHA production processes using cyanobacteria; these light-driven processes resort to photobioreactors similar to those used for microalgae cultivation and can be operated both discontinuously and continuously. This development is parallel to the emerging use of methane and syngas as abundantly available gaseous substrates, which also calls for bioreactor systems with optimized gas transfer. The review sheds light on the challenges of diverse PHA production processes in different bioreactor types and operational regimes using miscellaneous microbial production strains such as extremophilic Archaea, chemoheterotrophic eubacteria and phototrophic cyanobacteria. Particular emphasis is dedicated to the limitations and promises of different bioreactor-strain combinations and to efforts devoted to upscaling these processes to industrially relevant scales.

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Section: Batch Systemsmentioning

confidence: 99%

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Koller

2018

Fermentation

145

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“…optimized the upstream process of PHA production by mixing microbial cultures and feeding substrates as well as pH control during the fermentations process (Chen et al . ). Anoxic environment might also be economically viable for the PHA accumulation, as it accumulates higher PHA production (Davis et al .…”

Section: The Optimized Strategies Used In the Production Of Phasmentioning

confidence: 97%

Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms

Liu

Zhang

et al. 2015

Lett Appl Microbiol

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Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than the traditional synthetic materials has greatly limited the wide application of PHA. Here, we systematically summarized recent progress in production of PHAs and a series of optimization strategies such as supplying renewable carbon substrates, developing better bacterial strains, optimization of fermentation processes, engineering new pathways and etc., were applied to reduce production cost, therefore providing many new ideas and methods for the production of PHAs in economically viable processes. This is believed to be a comprehensive report to show different strategies and methods for low-cost production of PHAs. Further studies are still needed to make PHAs more and more economically viable to meet a wide range of applicability.

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“…At the moment, we witness increasing efforts to optimize the application of mixed microbial consortia (MMC) for PHA biosynthesis as inexpensive alternative to processes based on pure, monoseptic cultures. For such MMC-based processes, selective environmental pressure is exerted to a natural microbial consortium by employing suitable cultivation conditions [57][58][59][60][61][62][63][64]. As PHA are storage polymers naturally accumulated under dynamic environmental conditions, selective pressure to boost PHA biosynthesis can be achieved via so called "cyclic feast-and-famine feeding regimes", which consist of a series of alternating availability (feast) and absence of nutrients (famine) [64,65].…”

Section: Batch Systemsmentioning

confidence: 99%

“…In the context of MMC for PHA production, continuous feeding strategies are more and more implemented [117]; such processes allow PHA production with the additional benefit of a more stable Especially in the case of using toxic substrates like those usually needed for mcl-PHA biosynthesis, dual nutrient (carbon and nitrogen source) limited growth (DNL) in continuous cultures is suggested as a suitable method to produce different types of PHA. The concept of DNL originated from the biological law that growth rate, concentration and composition of biomass is not influenced by one limiting factor alone, but by the concerted action of two or more limiting nutrients (reviewed by [122]).…”

Section: Generalmentioning

confidence: 99%

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Koller¹

2018

Preprint

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHA) are microbial biopolyesters utilized as “green plastics”. Their production under controlled conditions resorts to bioreactors operated in different modes. Because PHA biosynthesis constitutes a multiphase process, both feeding strategy and bioreactor operation mode need smart adaptation. Traditional PHA production setups based on batch, repeated batch, fed-batch or cyclic fed-batch processes are often limited in productivity, or display insufficient controllability of polyester composition. For highly diluted substrate streams like it is the case for (agro)industrial waste streams, fed-batch enhanced by cell recycling were recently reported as a viable tool to increase volumetric productivity. As emerging trend, continuous fermentation processes in single-, two-, and multi-stage setups are reported, which bring the kinetics of both microbial growth and PHA accumulation into agreement with process engineering, and allow tailoring PHA´s molecular structure. Moreover, we currently witness an increasing number of CO2-based PHA production processes using cyanobacteria; these light-driven processes resort to photobioreactors similar to those used for microalgae cultivation, and can be operated both discontinuously and continuously. This development goes in parallel to the emerging use of methane and syngas as an abundantly available gaseous substrates, which also calls for bioreactor systems with optimized gas transfer. The review sheds light on the challenges of diverse PHA production processes in different bioreactor types and operational regimes using miscellaneous microbial production strains such as extremophilic Archaea, chemoheterotrophic eubacteria and phototrophic cyanobacteria. Particular emphasize is dedicated to the limitations and promises of different bioreactor-strain combinations, and to efforts devoted to upscaling these processes to industrially relevant scales.

show abstract

Efficient polyhydroxyalkanoate (PHA) accumulation by a new continuous feeding mode in three-stage mixed microbial culture (MMC) PHA production process

Cited by 41 publications

References 25 publications

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

Recent strategies for efficient production of polyhydroxyalkanoates by micro-organisms

A Review on Established and Emerging Fermentation Schemes for Microbial Production of Polyhydroxyalkanoate (PHA) Biopolyesters

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