Efficient plant regeneration of watermelon (Citrullus lanatus Thunb.) via somatic embryogenesis and assessment of genetic fidelity using ISSR markers

Vinoth, A.; Ravindhran, R.

doi:10.1007/s11627-015-9731-8

Cited by 19 publications

(9 citation statements)

References 40 publications

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“…Some previous studies have reported 100% homogeneity among plantlets regenerated from somatic embryos. These types include Bacopa monnieri (Khilwani et al, 2016), Citrullus lanatus (Vinoth and Ravindhran, 2016), and Abutilon indicum (Seth et al, 2017). Even though the walnut somatic embryos used in this study have been subcultured for more than 20 years, surprisingly, the ISSR profiles showed 100% genetic stability when the regenerated plantlets were compared with the cultivar of origin.…”

Section: Resultsmentioning

confidence: 89%

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Sadat-Hosseini¹,

Vahdati²,

Leslie³

2019

horts

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Somatic embryos (SEs) can play important roles in genetic manipulation and breeding. They can be used as targets for induced mutagenesis, as material for cryopreservation and germplasm conservation, and for transformation or gene editing in support of plant improvement and proof of gene function. However, germination rates of walnut (Juglans regia) SEs are low, and the genetic stability of plantlets regenerated from them has not been explored. Here, we studied first the effects of gibberellic acid (GA3) and low temperature storage (LTS) on germination of walnut somatic embryos. Second, we assessed the genetic fidelity of plantlets regenerated from these SEs by comparing them to each other and to their cultivar of origin. Results showed that GA3 and LTS increased the walnut SE germination rate. The best rate was observed when SEs were subjected to LTS for 60 d followed by culture on a medium with either 1 or 3 mg·L−1 GA3 (56.6% and 46.6% germination respectively). Genetic stability was evaluated, using flow cytometry and 15 sets of ISSR primers. Flow cytometry indicated that all samples (i.e., regenerated and parental counterpart) showed the same peak. Amplified fragments of inter simple sequence repeats (ISSR) primers ranged in size from ≈200 to 1800 bp. All ISSR profiles of regenerants were monomorphic. Results did not show any genetic differences among plantlets regenerated from SEs or from their parental counterpart. Due to this apparent genetic stability, walnut SEs can be useful for genetic transformation and germplasm conservation.

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Section: Resultsmentioning

confidence: 89%

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Sadat-Hosseini¹,

Vahdati²,

Leslie³

2019

horts

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“…Similar stages are also detected in Hypoxis hemerocallidea [45], Coriandrum sativum [40], and Citrullus lanatus Thunb. [46]. Our results show that those somatic embryos induced from embryo, cotyledon, and hypocotyl explants of P. ostii 'Fengdan' developed well into mature embryos on their respective basal media supplemented with both 3.0 mg·L −1 BA and 1.0 mg·L −1 NAA.…”

Section: Discussionmentioning

confidence: 59%

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Ren

Jeong

2019

Plants

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Somatic embryogenesis is a preferred method for vegetative propagation due to its high propagation efficiency. In this study, zygotic embryos, cotyledons, and hypocotyls of Paeonia ostii ‘Fengdan’ were used as the explant to induce somatic embryogenesis. The results showed that a combination of 0.5 mg·L−1 thidiazuron (TDZ) and 0.5 mg·L−1 2,4-dichlorophenoxyacetic acid (2,4-D) was effective in inducing somatic embryos from the zygotic embryo and cotyledon explants. Hypocotyls only formed somatic embryos on Murashige and Skoog (MS) medium supplemented with both 0.5 mg·L−1 TDZ and 0.5 mg·L−1 1-naphthylacetic acid (NAA). Moreover, the compact callus was effectively produced from zygotic embryo, cotyledon, and hypocotyl explants in medium supplemented with a combination of 3.0 mg·L−1 6-benzylaminopurine (BA) and 1.0 mg·L−1 NAA, and then converted into somatic embryos in the same medium, and the ratio of the explants with embryo induction and number of embryos induced per explant were much higher than those induced by 0.5 mg·L−1 TDZ and either 0.5 mg·L−1 2,4-D or 0.5 mg·L−1 NAA. The MS medium was better than the woody plant medium (WPM) for inducing somatic embryos from zygotic embryo and hypocotyl explants, whereas the WPM was better than the MS medium for somatic embryogenesis induction from cotyledon explants. All of the somatic embryos developed well into mature embryos on their respective media supplemented with both 3.0 mg·L−1 BA and 1.0 mg·L−1 NAA. Overall, the protocols for indirect somatic embryogenesis from zygotic embryo, cotyledon, and hypocotyl of P. ostii ‘Fengdan’ were successfully established, which can greatly facilitate their propagation and breeding processes.

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“…The values of genetic instability in this study (≤ 10 %) correspond to absent to low levels of variation reported for SE (0 -10 %) as in Cassia occidentalis, Ipomoea batatas, Picea abies, Distylium chinense, Quercus robur, Psidium guajava, Olea maderensis, O. europea, etc. (Fourré et al 1997, Lopes et al 2006, Valladares et al 2006, Triqui et al 2008, Yang et al 2008, Mehta et al 2011, Rai et al 2012, Bobadilla et al 2013, Li et al 2014, Carra et al 2016, Morais-Lino et al 2016, Naz et al 2016, Vinoth and Ravindhran 2016. It is possible that the explants selected may be less susceptible to genetic instability (DeVerno et al 1999) and, as a consequence, the results differ from those reported by others using the same morphogenetic route with intermediate levels of variation (17 -31 %) (Müller et al 1990, López et al 2004, Wilhelm et al 2005, Burg et al 2007, Prado et al 2010, Dey et al 2015.…”

Section: Resultsmentioning

confidence: 98%

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

Chávez-Cortázar¹,

Mata-Rosas²,

Oyama³

et al. 2020

Biologia plant.

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The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The embryogenic response was not significantly affected by the concentration of 2,4-D but did vary across cycles of induction. The addition of 4.52 μM 2,4-D induced the highest total number of embryos (100.5), the mean number of somatic embryos (25.1) and somatic embryos per explant (80.6). In both 2,4-D concentration (2.26 or 4.52 μM), genetic integrity between the donor and the propagated clones was 0.90, and the low genetic instability (≤ 0.10 in both PGR treatments) might be due to effect of cyclic somatic embryogenesis or the different response of the explants at stress in in vitro culture conditions. However, it is necessary to examine more cell lines and somatic embryogenesis cycles.

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Efficient plant regeneration of watermelon (Citrullus lanatus Thunb.) via somatic embryogenesis and assessment of genetic fidelity using ISSR markers

Cited by 19 publications

References 40 publications

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Germination of Persian Walnut Somatic Embryos and Evaluation of their Genetic Stability by ISSR Fingerprinting and Flow Cytometry

Enhanced Somatic Embryo Induction of a Tree Peony, Paeonia ostii ‘Fengdan’, by a Combination of 6-benzylaminopurine (BA) and 1-naphthylacetic Acid (NAA)

Induction of somatic embryogenesis and evaluation of genetic stability in regenerated plants of Magnolia dealbata

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