Efficient plant regeneration of Malaysian indica rice MR 219 and 232 via somatic embryogenesis system

Zuraida, A. R.; Naziah, B.; Zamri, Z.; Subramaniam, Sreeramanan

doi:10.1007/s11738-011-0739-3

Cited by 37 publications

(34 citation statements)

References 8 publications

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“…1 and 2). Zuraida et al 44 reported that CI frequency depends on genotype, and most indica rice cultivars had poor callusing potentiality. In this case we examined the CI potentiality using 4 hormonal combinations (H 1 , H 2 , H 3 and H 4 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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D number of viable callus; ICn D number of inoculated callus.Enhancement of callus induction and its regeneration efficiency through in vitro techniques has been optimized for 2 abiotic stresses (salt and air desiccation) using 3 rice genotypes viz. BR10, BRRI dhan32 and BRRI dhan47. The highest frequency of callus induction was obtained for BRRI dhan32 (64.44%) in MS medium supplemented with 2, 4-D (2.5 mgL ¡1) and Kin (1.0 mgL ¡1). Different concentrations of NaCl (2.9, 5.9, 8.8 and 11.7 gL ¡1 ) were used and its effect was recorded on the basis of viability of calli (VC), relative growth rate (RGR), tolerance index (TI) and relative water content (RWC). It was observed that in all cases BRRI dhan47 showed highest performance on tolerance to VC (45.33%), RGR (1.03%), TI (0.20%) and RWC (10.23%) with 11.7 gL ¡1 NaCl. Plant regeneration capability was recorded after partial air desiccation pretreatment to calli for 15, 30, 45 and 60 h. In this case BRRI dhan32 gave maximum number of regeneration (76.19%) when 4 weeks old calli were desiccated for 45 h. It was observed that air desiccation was 2-3 folds more effective for enhancing green plantlet regeneration compared to controls. Furthermore, desiccated calli also showed the better capability to survive in NaCl induced abiotic stress; and gave 1.9 fold (88.80%) increased regeneration in 11.7 gL ¡1 salt level for BRRI dhan47. Analysis of variance (ANOVA) showed that the genotypes, air desiccation and NaCl had significant effect on plant regeneration at P < 0.01.

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Section: Discussionmentioning

confidence: 99%

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Siddique

Ara

Islam

et al. 2014

Plant Signaling & Behavior

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“…Followed by rinsed three times in deionized water (Zuraida et al, 2012). Sterilized seeds were cultured on MS media (Murashige and Skoog, 1962) supplemented with 3% sucrose, 0.3% gelrite and 2 mg L -1 2, 4-D (pH 5.8) to induce the formation of MR219 callus.…”

Section: Seed Sterilization and Callus Inductionmentioning

confidence: 99%

Selection, Characterizations and Somatic Embryogenesis of Malaysian Salt-tolerant Rice (Oryza sativa cv. MR219) through Callogenesis

Kalhori¹,

Nulit²,

Go³

et al. 2017

IJAB

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Salt-tolerant lines of MR219 were produced through somatic embryogenesis from salt-tolerant callus of Oryza sativa L. cv.MR219 using in vitro selection procedure. Callus was developed aseptically from seeds on MS media supplemented with 2 mg L -1 2, 4-D. Then, callus directly was sub-cultured on MS media with different concentrations of NaCl (0, 50, 100, 200, and 300 mM) to produce salt-tolerant callus. Based on the callus characteristics which are morphological, physiological and biochemical cascades such as proline content, total protein, total soluble sugar, lipid peroxidation, activity of ascorbate peroxidase and catalase, salt-tolerant callus was screened and selected. After 4 months, callus cultured in 50 and 100 mM NaCl showed yellow color, soft, friable and nodular proliferating. However, callus cultured in 200 and 300 mM NaCl turn blackish-brown and stiff and acutely-necrotic. The selected salt-tolerant callus was sub-cultured on MS media for somatic embryogenesis. The salt-tolerant plantlets were transferred into pots individually for acclimatization purpose. Salt stress caused significant reduction in water content, fresh and dry weight of callus. The level of total soluble sugar, proline, lipid peroxidation and ascorbate peroxidase significantly increased under salt stress. Salt-tolerant callus indicated high activity of catalase that determined more protection against production of reactive oxygen species. According to growth performance and antioxidant capacity, the plantlets from 50 and 100 mM NaCl, selected as salt-tolerant line. This study suggests the methodology to produce salt-tolerant cultivar of rice which could be a step forward to commercialization.

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“…Seeds of various recalcitrant plant species like Indica rice may possess restrictions in promoting callus in vitro culture (Thokozani et al 2013). Efforts have been made to find appropriate rice explants to induce embryogenic calli under suitable in vitro conditions (Zuraida et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

Abiri

Mahmood

Shaharuddin

et al. 2017

Int. J. Environ. Sci. Technol.

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Enhancing of the efficient tissue culture protocol for somatic embryos would facilitate the engineered breeding plants program. In this report, we describe the reproducible protocol of Malaysian rice (Oryza sativa L.) cultivar MR219 through somatic embryogenesis. Effect of a wide spectrum of exogenesis materials was assessed in three phases, namely callogenesis, proliferation and regeneration. Initially, rice seeds were subjected under various auxin treatments. Secondly, the effect of different concentrations of 2,4-D on callus induction was evaluated. In the next step, the efficiency of different explants was identified. Subsequently, the effects of different auxins, cytokinins, L-proline, casein hydrolysate and potassium metasilicate concentrations on the callus proliferation and regeneration were considered. For the callogenesis phase, 2 mg L -1 of 2,4-D and roots were chosen as the best auxin and explant. In the callus proliferation stage, the highest efficiency was observed at week eight in the MS media supplemented with 2 mg

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Efficient plant regeneration of Malaysian indica rice MR 219 and 232 via somatic embryogenesis system

Cited by 37 publications

References 8 publications

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Effect of air desiccation and salt stress factors on in vitro regeneration of rice (Oryza sativaL.)

Selection, Characterizations and Somatic Embryogenesis of Malaysian Salt-tolerant Rice (Oryza sativa cv. MR219) through Callogenesis

Enhancing somatic embryogenesis of Malaysian rice cultivar MR219 using adjuvant materials in a high-efficiency protocol

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