Efficient plant regeneration in vitro from cotyledon explants of chieh-qua (Benincasa hispida Cogn. var. chieh-qua)

Abstract

An efficient plant regeneration system for Benincasa hispida Cogn. var. chieh-qua was developed using explants isolated from mature seeds germinated on moist filter paper under aseptic conditions for 3 days in the dark and 2 days under light. Shoot regeneration frequency (percentage of explant-forming shoots) was about 90% when the proximal cotyledon portion with a 1 mm-long hypocotyl segment was first cultured on Murashige and Skoog (MS) medium supplemented with 6.0 mg/l benzylaminopurine (BA) and 0.2 mg/l naphthalene acetic acid (NAA) for 4 days and then transferred to MS medium containing 1 mg/l BA for 4 weeks. About 80% of the regenerated shoots could elongate further. The root induction medium was half-strength MS medium with 0.01 mg/l NAA and 0.01 mg/l BA. The average rooting frequency was about 93%.

Cefotaxime and Benzyladenine Improve Melon Regeneration

“…With regards to the explant position, researchers have stated that young and undifferentiated cells in the proximal zone of hypocotyl may contribute to the rapidity of the regeneration rather than cotyledon section (13). This might be related to the differences in endogenous phytohormone levels in the plant tissues (14) or the meristemic activity of the plant tissue as well, which might be affected by plant growth regulators such as cytokinin (15). Moreover, shoot buds usually arise from the meristematic cells called meristemoids.…”

In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

“…Embryonic explants have been used for micropropagation and genetic transformation [13]. Direct shoot regeneration has been reported in many plants from zygotic embryo explant in Boscia Senegalensis [14], Cajanus cajan [15], from decapitated embryonic axes in Cicer arietinum [16], Clitoria ternatea [17] and cotyledons in Benincasa hispida [18] and Ricinus communis [19].…”