Efficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions

Wu, Siqin; Johansson, Jan; Hovatta, Outi; Rising, Anna

doi:10.1007/s00018-015-2053-5

Cited by 8 publications

(11 citation statements)

References 34 publications

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“…F 2 We get F 2 by the same technique as F 0 but here we dried the RSDS in air for 60 seconds. F 2 is very hard to dissolve in water and it is stable in both water and air.…”

Section: Fmentioning

confidence: 99%

“…Several researchers [2,[8][9][10][11][12] have found remarkable pH variation in these glands. They [2,[8][9][10][11][12] marked that these controlled variations stimulate certain portions of the SSs-protein, which initiates the production of RSDS in certain well localized (and recognized) point. Mimicking this known knowledge, one can produce industrial quantities of SSs.…”

Section: Introductionmentioning

confidence: 97%

“…SSs are nontoxic and have some medical applications such as them to be likely behaved to stimulate human immune cells [1]. Certain types of SSs prevents bacteria and fungi, allowing them to be ideal biomaterials for microsurgery and, even medical device applications [2,3]. Gatesy et al [4] are of the first to point out about the importance of SSs.…”

Section: Introductionmentioning

confidence: 99%

“…As a direct consequence, producing industrial rate should implicate the direct usage of human-made silk compounds with human spinning technologies. Researchers starts to mimic spiders and turn to biomimetic-spinning technologies [2,[8][9][10][11][12]. Up to date, they did not reach to their aim.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, one must think about stronger solvents that can solve this problem. In nature, before giving silk RSDSs, spider keep this protein (silk) in an aqueous solution in their silk glands; this protein is termed spidroin [2]. Several researchers [2,[8][9][10][11][12] have found remarkable pH variation in these glands.…”

Section: Introductionmentioning

confidence: 99%

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Preparation and Characterization of Artificial Spider Silk Produced through Microchannel Techniques

Abdalla¹,

Obaid²,

Al-Marzouki³

et al. 2017

J Material Sci Eng

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Spider silk (SS) is naturally tough; however, it turns soft when wet by water. Spiders produce high-quality silk threads by adjusting the molecular assemblage of SS-proteins and the arrangements structure of threads and recombinant spider dragline silk (RSDS). The general wet spinning techniques for producing recombinant spidroins results in uncorrected explanation to the natural spinning technique. In this study, we use tailored-SS with relative low molecular weight of 47 kD to produce a water-soluble RSDS protein. We built a microfluidic ship and used it to spun SS using aqueous solutions-micro-technique (wet spinning). This was done in order to mimic the spider-spinning processes using a steady post-spin drawing process. We succeeded to produce assemblies of spidroins with fibril structure. Then, compact constituting of micro-threads followed these wet spinning processes. Wet spinning was followed by improving the orientation, crystalline structure, and fibril melting of the hierarchical structure. The initial mechanical characterization (tensile strength) of the RSDSs attained about 510 MPa with respective extension 44%.

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“…F 2 We get F 2 by the same technique as F 0 but here we dried the RSDS in air for 60 seconds. F 2 is very hard to dissolve in water and it is stable in both water and air.…”

Section: Fmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Preparation and Characterization of Artificial Spider Silk Produced through Microchannel Techniques

Abdalla¹,

Obaid²,

Al-Marzouki³

et al. 2017

J Material Sci Eng

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Biomedical Applications of Recombinant Silk‐Based Materials

Aigner

DeSimone

Scheibel³

2018

Advanced Materials

240

203

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Silk is mostly known as a luxurious textile, which originates from silkworms first cultivated in China. A deeper look into the variety of silk reveals that it can be used for much more, in nature and by humanity. For medical purposes, natural silks were recognized early as a potential biomaterial for surgical threads or wound dressings; however, as biomedical engineering advances, the demand for high-performance, naturally derived biomaterials becomes more pressing and stringent. A common problem of natural materials is their large batch-to-batch variation, the quantity available, their potentially high immunogenicity, and their fast biodegradation. Some of these common problems also apply to silk; therefore, recombinant approaches for producing silk proteins have been developed. There are several research groups which study and utilize various recombinantly produced silk proteins, and many of these have also investigated their products for biomedical applications. This review gives a critical overview over of the results for applications of recombinant silk proteins in biomedical engineering.

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Influence of direct or indirect contact for the cytotoxicity and blood compatibility of spider silk

Kuhbier

Coger

Mueller

et al. 2017

J Mater Sci: Mater Med

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Spider silk became one of the most-researched biomaterials in the last years due to its unique mechanical strength and most favourable chemical composition for tissue engineering purposes. However, standardized analysis of cytocompatibility is missing. Therefore, the aim of this study was to investigate hemolysis, cytotoxicity of native spider silk as well as influences on the cell culture medium. Changes of cell culture medium composition, osmolarity as well as glucose and lactate content were determined via ELISA measurement. Possible hemolysis and cytotoxicity in vitro of spider silk were performed via measurement of hemoglobin release of human red blood cells or relative metabolic activity of L929 fibroblasts, respectively, according to international standard procedures. In ELISA measurement, no significant changes in medium composition could be found in this study. Spider silk was not hemolytic in direct and indirect testing. However, a borderline cytotoxicity according to definitions was found in indirect cytotoxicity testing. Nevertheless, in direct cytotoxicity testing, relative metabolic activity measurement revealed that spider silk is not cytotoxic under these conditions. This is the first study to conduct standardized tests regarding cytotoxicity and hemolysis of native spider silk, which might be considered inert in cell culture. As neither hemolysis nor cytotoxicity was found in direct contact in standardized procedures, safety in biomedical applications may be assumed. The indirect cytotoxicity seems to play a minor role in vivo. However, a borderline toxicity was revealed, suggesting potential leachables not yet identified. Displays one of the weaving frames used in this study after seeding with the single drop technique described herein.

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Efficient passage of human pluripotent stem cells on spider silk matrices under xeno-free conditions

Cited by 8 publications

References 34 publications

Preparation and Characterization of Artificial Spider Silk Produced through Microchannel Techniques

Preparation and Characterization of Artificial Spider Silk Produced through Microchannel Techniques

Biomedical Applications of Recombinant Silk‐Based Materials

Influence of direct or indirect contact for the cytotoxicity and blood compatibility of spider silk

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