Efficient One-Pot Synthesis of Uridine Diphosphate Galactose Employing a Trienzyme System
Yajing Li,
Qi Chen,
Siyao Liu
et al.
Abstract:The limited availability of high-cost
nucleotide sugars is a significant
constraint on the application of their downstream products (glycosides
and prebiotics) in the food or pharmaceutical industry. To better
solve the problem, this study presented a one-pot approach for the
biosynthesis of UDP-Gal using a thermophilic multienzyme system consisting
of GalK, UGPase, and PPase. Under optimal conditions, a 2 h reaction
resulted in a UTP conversion rate of 87.4%. In a fed-batch reaction
with Gal/ATP = 20 mM:10 mM… Show more
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.