Efficient Neutralization of Primary Isolates by the Plasma from HIV-1 Infected Indian Children

Abstract

We tested the plasma of 51 HIV-1-infected children (23 naïve and 28 ART treated) for neutralization against five primary isolates (PIs) generated from adult Indian HIV-1-infected patients. The plasma exhibited neutralization potential with significantly higher neutralizing antibody titers in ART-treated children than naïve children against three out of five PIs (p<0.0001). Further, in treated children, neutralizing antibody titers were higher in those children with suppressed viremia (<1000 RNA copies/mL) than non-suppressors against two of the three PIs. We report here for the first time the neutralization potential of the plasma of HIV-1-infected Indian children.

Diversity of HIV Type 1 Envelope (V3-V5) Sequence in HIV Type 1-Infected Indian Children

“…We recently reported the neutralization antibody response elicited in HIV-1-infected children against HIV-1 subtype C primary isolates. 4 We extend our findings in this study by assessing the sequence variability of the envelope (V3-V5 region) of the viruses infecting these children.…”

An HIV-1 Broadly Neutralizing Antibody from a Clade C-Infected Pediatric Elite Neutralizer Potently Neutralizes the Contemporaneous and Autologous Evolving Viruses

“…At the time of sampling in the present study (2015), the AIIMS_330 donor was antiretroviral (ART) naive; the CD4 ϩ T cell counts and plasma viral loads were 1,033 cells/l and 85,400 RNA copies/ml, respectively. The plasma antibody neutralization activity of this donor, against a few HIV-1 viruses, has been previously documented (37,40,41). In order to assess the evolution of antibody response in this donor, here we further characterized AIIMS_330 (2015) plasma against a large panel of heterologous HIV-1 tier 1, 2, and 3 pseudoviruses (n ϭ 33) (Fig.…”

An HIV-1 broadly neutralizing antibody from a clade C infected pediatric elite neutralizer potently neutralizes the contemporaneous and autologous evolving viruses

“…At the time of sampling (2015), he was antiretroviral (ART) naïve, the CD4 T cell counts and plasma viral loads were 1033 cells/µl and 85,400 RNA copies/ml respectively. The plasma antibody neutralization activity of AIIMS_330 donor against a few HIV-1 viruses has been previously documented (37, 39, 40). In order to assess the evolution of antibody response in this donor, we further characterized AIIMS_330 (2015) plasma against a large panel of heterologous HIV-1 tier 1, 2 and 3 pseudoviruses (n=33) (Figure 1A, Table S1).…”

“…This study describes for the first time, a pediatric bNAb AIIMS-P01 IgG1, isolated from single B cell of an ART naïve HIV-1C chronically infected pediatric LTNP AIIMS_330, and identified as an elite neutralizer in this study, based on plasma mapping analysis. This AIIMS_330 donor belonged to a rare cohort of vertically transmitted ART naïve HIV-1C infected children that have been followed up for more than a decade (37–40). The AIIMS-P01 bNAb isolated from this elite neutralizer showed comparable neutralization efficiency against clade C and B HIV-1 viruses thereby suggesting that this bNAb may neutralize major viral clades responsible for infection globally.…”